Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature
Abstract
:1. Introduction
1.1. Epidemiology
1.2. Pathogenesis
1.3. Diagnostic Tools
2. Case Report
3. Materials and Methods
3.1. Microbiological Culture and Microscopy
3.2. MALDI-TOF Method
3.3. PCR Amplification and DNA Sequencing
3.4. Search Strategy
3.5. Inclusion Criteria
3.6. Exclusion Criteria
4. Results
4.1. Culture-Based and Microscopy Identification of H. capsulatum from a Bronchoalveolar Lavage Sample
4.2. MALDI-TOF MS-Based Identification of H. capsulatum
4.3. Identification of H. capsulatum by Sequencing PCR-Amplified DNA
4.4. Literature Review of MALDI-TOF-Based Identification of H. capsulatum
5. Discussion
6. Conclusions and Perspectives
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
AIDS | Acquired Immunodeficiency Syndrome |
AFST | Antifungal Susceptibility Testing |
BAL | Bronchoalveolar Lavage |
BHI | Brain Heart Infusion |
CBA | Chocolate Blood Agar |
CD4 | Cluster of Differentiation 4 |
ELISA | Enzyme-Linked Immunosorbent Assay |
FDA | Food and Drug Administration |
HCCA | α-cyano-4- hydroxycinnamic acid |
HIV | Human Immunodeficiency Virus |
HLPC | Health laboratory Practitioners Council |
IBD | Inflammatory Bowel Disease |
LSU | Large Subunit |
LTCD4+ | T CD4+ Lymphocyte |
MALDI-TOF MS | Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry |
MIC | Minimal Inhibitory Concentration |
PCR | Polymerase Chain Reaction |
SDA | Sabouraud Dextrose Agar |
TFA | Trifluoroacetic Acid |
TNF | Tumor Necrosis Factor |
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MALDI Database Version | Numbers of Strains | MALDI Score Identification | Phase b | |
---|---|---|---|---|
Panda et al. 2015 [29] | MALDI Biotyper 3.1 database, Bruker Daltonics | 1 | NA- H. capsulatum was not available in the database | NA |
Valero et al. 2018 [28] | MALDI Biotyper Database v.4.0.0.1, | 6 | 1.78 ± 0.15 | 3 M |
Bruker Daltonics | 3 M/Y | |||
Rychert et al. 2018 [30] | Vitek MS v3.0 | 32 | Over 90% correct identification at the genus level | NA |
Le et al. 2021 [31] | NA a | 1 | NA | NA |
Velayuthan et al. 2020 [32] | NA a | 1 | NA | NA |
Montagnac et al. 2019 [33] | NAa | 1 | NA | NA |
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Cosio, T.; Gaziano, R.; Fontana, C.; Pistoia, E.S.; Petruccelli, R.; Favaro, M.; Pica, F.; Minelli, S.; Bossa, M.C.; Altieri, A.; et al. Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature. J. Fungi 2023, 9, 1019. https://doi.org/10.3390/jof9101019
Cosio T, Gaziano R, Fontana C, Pistoia ES, Petruccelli R, Favaro M, Pica F, Minelli S, Bossa MC, Altieri A, et al. Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature. Journal of Fungi. 2023; 9(10):1019. https://doi.org/10.3390/jof9101019
Chicago/Turabian StyleCosio, Terenzio, Roberta Gaziano, Carla Fontana, Enrico Salvatore Pistoia, Rosalba Petruccelli, Marco Favaro, Francesca Pica, Silvia Minelli, Maria Cristina Bossa, Anna Altieri, and et al. 2023. "Closing the Gap in Proteomic Identification of Histoplasma capsulatum: A Case Report and Review of Literature" Journal of Fungi 9, no. 10: 1019. https://doi.org/10.3390/jof9101019