A Polyphasic Approach including Whole Genome Sequencing Reveals Paecilomyces paravariotii sp. nov. as a Cryptic Sister Species to P. variotii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Whole Genome Sequencing and Assembly
2.2. Properties of P. paravariotii in Culture
2.3. Phylogenetic Analyses
2.4. Metabolite Analysis Using High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry
2.5. Sexual Crosses
2.6. Transformation
2.7. Microscopy
3. Results and Discussion
3.1. ‘Paecilomyces variotii’ Strain FRR 5287 Is Phylogenetically Separated from Previously Described Paecilomyces Species
3.2. Genome-Wide BLAST Comparisons Confirm Divergence between P. variotii and P. paravariotii and Reveal a Previously Unrecognised Horizontal Gene Transfer (HGT) Event
3.3. FRR 5287 Is Unable to Form Ascospores with a Fertile P. variotii Strain of Opposite Mating Type
3.4. Strain FRR 5287 Is Chemically Distinguished from P. variotii Due to Genetic Differences in Viriditoxin Biosynthesis
3.5. Features and Taxonomic Description of Paecilomyces Paravariotii
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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GenBank Accession | GenBank Name | Corrected Name | Citation |
---|---|---|---|
JAPVCG000000000 | P. fulvus FRR 3794 | - | This study |
JAPVCF000000000 | P. dactylethromorphus FRR 0699 | - | This study |
JAPVCE000000000 | P. dactylethromorphus FRR 5262 | - | This study |
JAPVCD000000000 | P. lecythidis FRR 3797 | - | This study |
JAPVCC000000000 | P. lecythidis FRR 4481 | - | This study |
JAPVCB000000000 | P. formosus FRR 3793 | - | This study |
JAPVCA000000000 | P. maximus FRR 4742 | - | This study |
JAPVBZ000000000 | P. maximus FRR 2337 | - | This study |
JAPVBY000000000 | P. maximus FRR 4140 | - | This study |
JANCMQ000000000 | P. variotii FRR 1658 | - | [24] |
JANCMP000000000 | P. variotii FRR 2889 | - | [24] |
JANCMO000000000 | P. variotii FRR 3823 | - | [24] |
JANCMN000000000 | P. variotii FRR 5287 | P. paravariotii FRR 5287 | [16] |
RHLL00000000.1 | P. variotii CBS 144490 | - | [16] |
RCNU00000000.1 | P. variotii CBS 101075 | - | [16] |
MSJH00000000.2 | Byssochlamys sp. IMV 00236 | P. variotii IMV 00236 | [25] (Cladosporium cladosporioides IMV 00236 in publication) |
QBDR00000000.1 | Thermoascaceae sp. COH1141 | P. lecythidis COH1141 | [26] |
JAGJCD000000000.1 | Aspergillus sp. MCCF 102 | P. lecythidis MCCF 102 | |
BAUL01000000.1 | Byssochlamys spectabilis No. 5 | P. formosus no. 5 | [27] |
JACXGS000000000.1 | Paecilomyces variotii WS011 | P. formosus WS011 | |
PNEM00000000.1 | Byssochlamys sp. AF001 | P. dactylethromorphus AF001 | [28] |
QEIL00000000.1 | Paecilomyces niveus Cornell no. 7 | - | [29] |
RCHW01000000.1 | Paecilomyces variotii MTDF-01 | - | [30] |
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Urquhart, A.S.; Idnurm, A. A Polyphasic Approach including Whole Genome Sequencing Reveals Paecilomyces paravariotii sp. nov. as a Cryptic Sister Species to P. variotii. J. Fungi 2023, 9, 285. https://doi.org/10.3390/jof9030285
Urquhart AS, Idnurm A. A Polyphasic Approach including Whole Genome Sequencing Reveals Paecilomyces paravariotii sp. nov. as a Cryptic Sister Species to P. variotii. Journal of Fungi. 2023; 9(3):285. https://doi.org/10.3390/jof9030285
Chicago/Turabian StyleUrquhart, Andrew S., and Alexander Idnurm. 2023. "A Polyphasic Approach including Whole Genome Sequencing Reveals Paecilomyces paravariotii sp. nov. as a Cryptic Sister Species to P. variotii" Journal of Fungi 9, no. 3: 285. https://doi.org/10.3390/jof9030285
APA StyleUrquhart, A. S., & Idnurm, A. (2023). A Polyphasic Approach including Whole Genome Sequencing Reveals Paecilomyces paravariotii sp. nov. as a Cryptic Sister Species to P. variotii. Journal of Fungi, 9(3), 285. https://doi.org/10.3390/jof9030285