Ras2 Is Responsible for the Environmental Responses, Melanin Metabolism, and Virulence of Botrytis cinerea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains and Culture Conditions
2.2. Generation of Gene Deletion Mutants
2.3. Phenotype Analysis
2.4. Virulence Assays
2.5. RNA Sequencing
2.6. Gene Expression Analysis
2.7. Statistical Analysis
3. Results
3.1. Bcras2 Is Required for Mycelial Growth and Development
3.2. Bcras2 Is Responsible for Oxidative Stress Responses and Cell Wall Integrity
3.3. Bcras2 Is Essential for Virulence
3.4. Identification of the Potential Downstream Targets of Bcras2 by RNA Sequencing
3.5. Validation of the Differentially Expressed Genes by RT-qPCR
3.6. The Melanin Metabolic Pathway Is Regulated by Bcras2
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Li, H.; Shen, X.; Wu, W.; Zhang, W.; Wang, Y. Ras2 Is Responsible for the Environmental Responses, Melanin Metabolism, and Virulence of Botrytis cinerea. J. Fungi 2023, 9, 432. https://doi.org/10.3390/jof9040432
Li H, Shen X, Wu W, Zhang W, Wang Y. Ras2 Is Responsible for the Environmental Responses, Melanin Metabolism, and Virulence of Botrytis cinerea. Journal of Fungi. 2023; 9(4):432. https://doi.org/10.3390/jof9040432
Chicago/Turabian StyleLi, Hua, Xuemei Shen, Wenjia Wu, Wanyu Zhang, and Yousheng Wang. 2023. "Ras2 Is Responsible for the Environmental Responses, Melanin Metabolism, and Virulence of Botrytis cinerea" Journal of Fungi 9, no. 4: 432. https://doi.org/10.3390/jof9040432