Regulation of Intracellular Reactive Oxygen Species Levels after the Development of Phallus rubrovolvatus Rot Disease Due to Trichoderma koningii Mycoparasitism
Abstract
:1. Introduction
2. Materials and Methods
2.1. Field Investigation of Phallus rubrovolvatus Rot Disease
2.2. Isolation and Identification of Pathogenic Fungi and Evaluation of Their Pathogenicity
2.3. Species Identification and Confrontation Testing of Rot Disease Pathogenic Fungi
2.4. Scanning Electron Microscopy (SEM)
2.5. Transmission Electron Microscopy (TEM)
2.6. Phallus rubrovolvatus Enzyme Activity Assays
2.7. Tissue ROS Levels
3. Results
3.1. Onset and Symptoms of Phallus rubrovolvatus Rot Disease
3.2. Isolation of the Pathogenic Fungus and Evaluation of Its Pathogenicity
3.3. Identification and Characterization of Fungal Isolates
3.4. Mycoparasitism Assays In Vitro
3.5. Morphological, Ultrastructural and Physiological Changes Process of Trichoderma koningii-Infested Phallus rubrovolvatus
3.5.1. Morphological Studies by SEM
3.5.2. Ultrastructural Studies by TEM
3.5.3. Effect on Defense Enzyme Activity
3.5.4. Changes in ROS Levels
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Greenhouse Planting | Undergrowth Planting | |
---|---|---|
Growing Area | Average Incidence (%) | |
Xingyi | 66.33 ± 4.16 | 15.33 ± 9.02 |
Nayong | 69.00 ± 14.42 | 36.00 ± 12.49 |
Qianxi | 80.33 ± 12.22 | 36.00 ± 4.00 |
Guiyang | 67.67 ± 3.06 | 6.00 ± 2.00 |
Guiding | 41.67 ± 4.16 | 10.00 ± 2.00 |
Undergrowth Planting | Greenhouse Planting | |||||
---|---|---|---|---|---|---|
Growing Area | Mean Minimum Temperature/°C | Mean Maximum Temperature/°C | Average Monthly Temperature/°C | Average Humidity % | Average Monthly Temperature/°C | Average Humidity % |
Qianxi | 17.73 | 27.60 | 22.67 | 78.70 | 29.50 | 85.20 |
Nayong | 17.70 | 26.83 | 22.27 | 80.50 | 28.30 | 90.30 |
Xingyi | 18.57 | 28.47 | 23.52 | 84.43 | 30.20 | 95.00 |
Guiding | 18.30 | 28.37 | 23.33 | 84.70 | 29.80 | 90.00 |
Guiyang | 18.17 | 27.40 | 22.78 | 79.73 | 28.50 | 85.40 |
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Lu, M.; Wen, T.; Guo, M.; Li, Q.; Peng, X.; Zhang, Y.; Lu, Z.; Wang, J.; Xu, Y.; Zhang, C. Regulation of Intracellular Reactive Oxygen Species Levels after the Development of Phallus rubrovolvatus Rot Disease Due to Trichoderma koningii Mycoparasitism. J. Fungi 2023, 9, 525. https://doi.org/10.3390/jof9050525
Lu M, Wen T, Guo M, Li Q, Peng X, Zhang Y, Lu Z, Wang J, Xu Y, Zhang C. Regulation of Intracellular Reactive Oxygen Species Levels after the Development of Phallus rubrovolvatus Rot Disease Due to Trichoderma koningii Mycoparasitism. Journal of Fungi. 2023; 9(5):525. https://doi.org/10.3390/jof9050525
Chicago/Turabian StyleLu, Meiling, Tingchi Wen, Ming Guo, Qihua Li, Xingcan Peng, Yan Zhang, Zhenghua Lu, Jian Wang, Yanjun Xu, and Chao Zhang. 2023. "Regulation of Intracellular Reactive Oxygen Species Levels after the Development of Phallus rubrovolvatus Rot Disease Due to Trichoderma koningii Mycoparasitism" Journal of Fungi 9, no. 5: 525. https://doi.org/10.3390/jof9050525