Transcriptomic Response of Clonostachys rosea Mycoparasitizing Rhizoctonia solani
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains
2.2. Sample Collection
2.3. cDNA Library Construction and Transcriptome Sequencing
2.4. Transcriptome Data Processing
2.5. DEG Analysis
2.6. Comparative Transcriptome Analysis
2.7. Quantitative Real-Time PCR Validation
3. Results
3.1. Transcriptome Sequencing and Data Processing
3.2. DEG Analysis
3.3. GO Enrichment Analysis
3.4. KEGG Enrichment Analysis
3.5. Screening of Potential Mycoparasitism-Related Genes
3.6. Comparative Transcriptomic Analysis
3.7. Quantitative Real-Time PCR Validation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Annotation | Gene Name | Isolate | Accession No. |
---|---|---|---|
ABC transporter | Taabc2 | T. atroviride | AY911669 |
β-1,3-glucanase | TvBng2 | T. virens | AF395756 |
Exo-β-1,3-glucanase | tag83 | T. asperellum | EU314718 |
β-1,3-glucanase | - | Trichoderma spp. | KJ603460 |
Subtilisin-like serine protease | TghSS42 | T. ghanense | KJ740359 |
Subtilisin-like protease | SS10 | T. harzianum | EF063644 |
Serine protease | tvsp1 | T. virens | AY242844 |
Chitinase | chit42 | T. virens | AF050098 |
Chitinase | chit33 | T. harzianum | X80006 |
Chitinase | chi18-10 | T. atroviride | DQ068748 |
Chitinase | chi18-13 | T. atroviride | DQ068752 |
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Sun, Z.-B.; Yu, S.-F.; Sun, M.-H.; Li, S.-D.; Hu, Y.-F.; Song, H.-J. Transcriptomic Response of Clonostachys rosea Mycoparasitizing Rhizoctonia solani. J. Fungi 2023, 9, 818. https://doi.org/10.3390/jof9080818
Sun Z-B, Yu S-F, Sun M-H, Li S-D, Hu Y-F, Song H-J. Transcriptomic Response of Clonostachys rosea Mycoparasitizing Rhizoctonia solani. Journal of Fungi. 2023; 9(8):818. https://doi.org/10.3390/jof9080818
Chicago/Turabian StyleSun, Zhan-Bin, Shu-Fan Yu, Man-Hong Sun, Shi-Dong Li, Ya-Feng Hu, and Han-Jian Song. 2023. "Transcriptomic Response of Clonostachys rosea Mycoparasitizing Rhizoctonia solani" Journal of Fungi 9, no. 8: 818. https://doi.org/10.3390/jof9080818
APA StyleSun, Z. -B., Yu, S. -F., Sun, M. -H., Li, S. -D., Hu, Y. -F., & Song, H. -J. (2023). Transcriptomic Response of Clonostachys rosea Mycoparasitizing Rhizoctonia solani. Journal of Fungi, 9(8), 818. https://doi.org/10.3390/jof9080818