Molecular Detection of Candida auris Using DiaSorin Molecular Simplexa® Detection Kit: A Diagnostic Performance Evaluation
Abstract
:1. Introduction
2. Methods and Results
3. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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C. auris Dilution | C. auris (FAM) | SEAC (Q670) | C. auris (FAM) | SEAC (Q670) | C. auris (FAM) | SEAC (Q670) |
---|---|---|---|---|---|---|
2.66 × 106 CFU/µL | 21.6 | 31.4 | 22.6 | 32.6 | 22.5 | 31.6 |
2.66 × 105 CFU/µL | 25.2 | 30.3 | 25.5 | 31.5 | 25.5 | 30.5 |
2.66 × 104 CFU/µL | 29.2 | 30.6 | 29.9 | 31.4 | 29.1 | 30.7 |
2.66 × 103 CFU/µL | 34 | 31.4 | 33 | 32.1 | 32.7 | 31 |
2.66 × 102 CFU/µL | 36.7 | 31.4 | 36.3 | 32.2 | 36.5 | 31.2 |
2.66 × 101 CFU/µL | 0 | 31.7 | 0 | 32.1 | 0 | 31.3 |
2.66 × 100 CFU/µL | 0 | 31.5 | 0 | 32.4 | 0 | 31.5 |
2.66 × 10−1 CFU/µL | 0 | 31.5 | 0 | 32.3 | 0 | 31.5 |
2.66 × 10−2 CFU µL | 0 | 31.3 | 0 | 31.9 | 0 | 31.2 |
2.66 × 10−3 CFU/µL | 0 | 31.5 | 0 | 32.1 | 0 | 31.2 |
Sample ID | Organism | C. auris (FAM) | SEAC (Q670) | Result |
---|---|---|---|---|
CAIDNA001 | Staphylococcus aureus | NaN | 31.5 | Not Detected |
CAIDNA002 | Moraxella catarrhalis | NaN | 31.7 | Not Detected |
CAIDNA003 | Nocardia farcinica | NaN | 31.6 | Not Detected |
CAIDNA004 | Streptococcus immitis | NaN | 31.7 | Not Detected |
CAIDNA005 | Candida lusitaniae | NaN | 31.3 | Not Detected |
CAIDNA006 | Candida kefyr | NaN | 31.8 | Not Detected |
CAIDNA007 | Candida tropicalis | NaN | 31.7 | Not Detected |
CAIDNA008 | Candida guillermondi | NaN | 32.4 | Not Detected |
CAIDNA009 | Candida krusei | NaN | 32.5 | Not Detected |
CAIDNA010 | Candida parapsilosis | NaN | 31.6 | Not Detected |
CAIDNA011 | Candida glabrata | NaN | 31.5 | Not Detected |
CAIDNA012 | Phaeoanellomyces werneckii | NaN | 31.5 | Not Detected |
CAIDNA014 | Tricophyton rubrum | NaN | 32.1 | Not Detected |
CAIDNA015 | T. tonsurans | NaN | 32 | Not Detected |
CAIDNA016 | Fusarium | NaN | 32.1 | Not Detected |
CAIDNA019 | Penicillium | NaN | 31.6 | Not Detected |
CAIDNA020 | Aspergillus niger | NaN | 31.5 | Not Detected |
CAIDNA021 | Propionibacterium acnes | NaN | 31.6 | Not Detected |
CAIDNA022 | Bacillus cereus | NaN | 32 | Not Detected |
CAIDNA023 | M. abscessus | NaN | 31.6 | Not Detected |
CAIDNA024 | M. fortuitum | NaN | 31.9 | Not Detected |
CAIDNA025 | MAC | NaN | 32.3 | Not Detected |
CAIDNA026 | Mycobacterium chelonae | NaN | 31.4 | Not Detected |
CAIDNA027 | Enterococcus faecium | NaN | 31.6 | Not Detected |
Number | Sample ID | C. auris (FAM) Ct | SEAC (Q670) Ct | PCR Result | Culture Result |
---|---|---|---|---|---|
1 | CA001 | 31.5 | 31 | Detected | Detected |
2 | CA002 | 32.1 | 29.5 | Detected | Detected |
3 | CA003 | 26.8 | 28.2 | Detected | Detected |
4 | CA004 | 36.6 | 28.3 | Detected | Detected |
5 | CA005 | 29.6 | 27.7 | Detected | Detected |
6 | CA006 | 28.3 | 26.1 | Detected | Detected |
7 | CA007 | 28.1 | 27.4 | Detected | Detected |
8 | CA008 | 25.9 | 29.3 | Detected | Detected |
9 | CA009 | 39 | 26.8 | Detected | Detected |
10 | CA010 | 26.1 | 27.4 | Detected | Detected |
11 | CA011 | 24.2 | 26.9 | Detected | Detected |
12 | CA012 | 33.9 | 27.9 | Detected | Detected |
13 | CA014 | 30.6 | 28.6 | Detected | Detected |
14 | CA016 | 22.4 | 28.1 | Detected | Detected |
15 | CA022 | 25 | 27.5 | Detected | Detected |
16 | CA023 | 21.2 | 28.2 | Detected | Detected |
17 | CA024 | 37.4 | 28.9 | Detected | Detected |
18 | CA025 | 23.2 | 27.5 | Detected | Detected |
19 | CA026 | 37.6 | 30.1 | Detected | Detected |
20 | CA047 | 22.4 | 27.6 | Detected | Detected |
21 | CA059 | 23.1 | 29.6 | Detected | Detected |
22 | CA060 | 19.3 | 31.3 | Detected | Detected |
23 | CA061 | 26.5 | 30 | Detected | Detected |
24 | CA062 | 20.2 | 31.6 | Detected | Detected |
25 | CA063 | 32.5 | 29.7 | Detected | Detected |
26 | CA064 | 28.3 | 29.5 | Detected | Detected |
27 | CA065 | 23 | 29.7 | Detected | Detected |
28 | CA066 | 26 | 29.7 | Detected | Detected |
29 | CA070 | 20.3 | 31.3 | Detected | Detected |
30 | CA077 | 28.1 | 29.6 | Detected | Detected |
31 | CA082 | 30.9 | 29.5 | Detected | Detected |
32 | CA021 | NaN | 28.3 | Not Detected | Not Detected |
33 | CA027 | NaN | 28 | Not Detected | Not Detected |
34 | CA028 | NaN | 27.8 | Not Detected | Not Detected |
35 | CA029 | NaN | 28 | Not Detected | Not Detected |
36 | CA030 | NaN | 27.9 | Not Detected | Not Detected |
37 | CA031 | NaN | 28 | Not Detected | Not Detected |
38 | CA032 | NaN | 29.2 | Not Detected | Not Detected |
39 | CA033 | NaN | 27.3 | Not Detected | Not Detected |
40 | CA034 | NaN | 27.8 | Not Detected | Not Detected |
41 | CA035 | NaN | 27.5 | Not Detected | Not Detected |
42 | CA036 | NaN | 27.6 | Not Detected | Not Detected |
43 | CA037 | NaN | 27.8 | Not Detected | Not Detected |
44 | CA038 | NaN | 27.9 | Not Detected | Not Detected |
45 | CA039 | NaN | 27.8 | Not Detected | Not Detected |
46 | CA040 | NaN | 28 | Not Detected | Not Detected |
47 | CA041 | NaN | 29.6 | Not Detected | Not Detected |
48 | CA042 | NaN | 27.6 | Not Detected | Not Detected |
49 | CA043 | NaN | 27.5 | Not Detected | Not Detected |
50 | CA044 | NaN | 28 | Not Detected | Not Detected |
51 | CA045 | NaN | 27.9 | Not Detected | Not Detected |
52 | CA046 | NaN | 29.6 | Not Detected | Not Detected |
53 | CA049 | NaN | 27.8 | Not Detected | Not Detected |
54 | CA050 | NaN | 27.7 | Not Detected | Not Detected |
55 | CA051 | NaN | 29.5 | Not Detected | Not Detected |
56 | CA055 | NaN | 29.8 | Not Detected | Not Detected |
57 | CA068 | NaN | 29.7 | Not Detected | Not Detected |
58 | CA067 | NaN | 29.8 | Not Detected | Not Detected |
59 | CA069 | NaN | 29.4 | Not Detected | Not Detected |
60 | CA071 | NaN | 29.3 | Not Detected | Not Detected |
61 | CA072 | NaN | 29.8 | Not Detected | Not Detected |
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Ramírez, J.D.; Wang, C.Y.; Bolton, D.; Liggayu, B.; Schaefer, S.; Patel, G.; Javaid, W.; Cordon-Cardo, C.; Firpo-Betancourt, A.; Sordillo, E.M.; et al. Molecular Detection of Candida auris Using DiaSorin Molecular Simplexa® Detection Kit: A Diagnostic Performance Evaluation. J. Fungi 2023, 9, 849. https://doi.org/10.3390/jof9080849
Ramírez JD, Wang CY, Bolton D, Liggayu B, Schaefer S, Patel G, Javaid W, Cordon-Cardo C, Firpo-Betancourt A, Sordillo EM, et al. Molecular Detection of Candida auris Using DiaSorin Molecular Simplexa® Detection Kit: A Diagnostic Performance Evaluation. Journal of Fungi. 2023; 9(8):849. https://doi.org/10.3390/jof9080849
Chicago/Turabian StyleRamírez, Juan David, Chin Yi Wang, Deandra Bolton, Bernadette Liggayu, Sarah Schaefer, Gopi Patel, Waleed Javaid, Carlos Cordon-Cardo, Adolfo Firpo-Betancourt, Emilia Mia Sordillo, and et al. 2023. "Molecular Detection of Candida auris Using DiaSorin Molecular Simplexa® Detection Kit: A Diagnostic Performance Evaluation" Journal of Fungi 9, no. 8: 849. https://doi.org/10.3390/jof9080849