A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer
Abstract
:1. Introduction
2. Results and Discussion
2.1. Histological Analysis of Neural Invasion Models Using Hematoxylin and Eosin Staining
2.2. Histochemical Assessment of Extracellular Matrix Components in Neural Invasion Models
2.3. Analysis of Cell Proliferation in Neural Invasion Models
2.4. Phenotypic Characterization of Nerve-Derived Cells in Neural Invasion Models
3. Conclusions
4. Materials and Methods
4.1. Cell Sources and Sample Acquisition
4.2. Generation of Neural Invasion Models
4.3. Histological Characterization of Neural Invasion Models
4.4. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antibody/Reagent | Dilution | Pretreatment | Reference |
---|---|---|---|
Mouse anti-human PCNA (Clone PC10) | Ready to use | EDTA, pH = 8, 95 °C for 25 min | Master Diagnostica, Granada, Spain (ref. MAD-000903QD) |
Rabbit anti-cytokeratin 5 | Ready to use | Citrate buffer pH = 6 95 °C for 25 min | Master Diagnostica, Granada, Spain (MAD-000491QD) |
Rabbit polyclonal anti-S100 antibody (Z0311) | 1: 400 | Citrate buffer pH = 6 30 min at 95 °C | DakoCytomation, Glostrup, Denmark (ref. Z0311) |
Mouse anti-vimentin monoclonal clone V9 | 1:200 | Citrate buffer pH = 6 25 min at 95 °C | Merck. St. Louis, MO, USA (ref. V6389) |
ImmPRESS® HRP Anti-Mouse IgG (Peroxidase) | Ready to use | - | Vector Laboratories. Burlingame, USA (ref. MP-7402) |
ImmPRESS® HRP Anti-Rabbit IgG (Peroxidase) | Ready to use | - | Vector Laboratories. Burlingame, USA (ref. MP-7401) |
Chromogen: Diaminobenzidine ready to use kit | - | - | Vector Laboratories. Burlingame, USA (ref. SK-4100) |
Counterstaining: Harris Hematoxylin | 30 s | - | Thermo Scientific. Runcorn, UK (ref. 6765004) |
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Ávila-Fernández, P.; Etayo-Escanilla, M.; Sánchez-Porras, D.; Blanco-Elices, C.; Campos, F.; Carriel, V.; García-García, Ó.D.; Chato-Astrain, J. A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer. Gels 2024, 10, 252. https://doi.org/10.3390/gels10040252
Ávila-Fernández P, Etayo-Escanilla M, Sánchez-Porras D, Blanco-Elices C, Campos F, Carriel V, García-García ÓD, Chato-Astrain J. A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer. Gels. 2024; 10(4):252. https://doi.org/10.3390/gels10040252
Chicago/Turabian StyleÁvila-Fernández, Paula, Miguel Etayo-Escanilla, David Sánchez-Porras, Cristina Blanco-Elices, Fernando Campos, Víctor Carriel, Óscar Darío García-García, and Jesús Chato-Astrain. 2024. "A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer" Gels 10, no. 4: 252. https://doi.org/10.3390/gels10040252