Green In Situ Synthesis of Silver Nanoparticles-Peptide Hydrogel Composites: Investigation of Their Antibacterial Activities
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation of AgNPs/Hydrogel Composites
2.2. Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-OES)
2.3. Dynamic Light Scattering (DLS)
2.4. SEM Measurement
2.5. X-ray Photoelectron Spectroscopy (XPS)
2.6. X-ray Small Angle Scattering (SAXS)
2.7. Rheological Studies
2.8. Swelling Ability
2.9. Antibacterial Studies
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Biosynthesis of Hydrogel Composites
4.3. UV-Vis Spectroscopy and Dynamic Light Scattering (DLS)
4.4. Electron Microscopy Studies
4.5. X-ray Photoelectron Spectroscopy (XPS)
4.6. Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES)
4.7. Small Angle X-ray Scattering (SAXS)
4.8. Rheological Measurements
4.9. Swelling Ability
4.10. Test Microorganisms Used in This Study
4.11. Plate Inhibition Zone Assay to Test for Antimicrobial Activity
4.12. Determination of Minimal Inhibitory Concentration (MIC)
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Samples | Swelling Degree (q) |
---|---|
hgel | 62.18 ± 0.35 |
AgNPs/hgel | 75.99 ± 0.65 |
AgNPs/hgel-honey | 79.11 ± 0.23 |
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Binaymotlagh, R.; Del Giudice, A.; Mignardi, S.; Amato, F.; Marrani, A.G.; Sivori, F.; Cavallo, I.; Di Domenico, E.G.; Palocci, C.; Chronopoulou, L. Green In Situ Synthesis of Silver Nanoparticles-Peptide Hydrogel Composites: Investigation of Their Antibacterial Activities. Gels 2022, 8, 700. https://doi.org/10.3390/gels8110700
Binaymotlagh R, Del Giudice A, Mignardi S, Amato F, Marrani AG, Sivori F, Cavallo I, Di Domenico EG, Palocci C, Chronopoulou L. Green In Situ Synthesis of Silver Nanoparticles-Peptide Hydrogel Composites: Investigation of Their Antibacterial Activities. Gels. 2022; 8(11):700. https://doi.org/10.3390/gels8110700
Chicago/Turabian StyleBinaymotlagh, Roya, Alessandra Del Giudice, Silvano Mignardi, Francesco Amato, Andrea Giacomo Marrani, Francesca Sivori, Ilaria Cavallo, Enea Gino Di Domenico, Cleofe Palocci, and Laura Chronopoulou. 2022. "Green In Situ Synthesis of Silver Nanoparticles-Peptide Hydrogel Composites: Investigation of Their Antibacterial Activities" Gels 8, no. 11: 700. https://doi.org/10.3390/gels8110700