Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate

Huang, Yuye; Xu, Kailei; Liu, Jingyi; Dai, Guangli; Yin, Jun; Wei, Peng

doi:10.3390/gels8020084

Open AccessArticle

Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate

by

Yuye Huang

^1,2,†

,

Kailei Xu

^1,2,3,4,†,

Jingyi Liu

^3,4,†,

Guangli Dai

⁵,

Jun Yin

^3,4,*

and

Peng Wei

^1,*

¹

Department of Plastic and Reconstructive Surgery, Ningbo First Hospital, Ningbo 315010, China

²

Center for Medical and Engineering Innovation, Central Laboratory, Ningbo First Hospital, Ningbo 315010, China

³

The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China

⁴

Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China

⁵

Department of Medical Engineering, Ningbo First Hospital, Ningbo 315010, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Gels 2022, 8(2), 84; https://doi.org/10.3390/gels8020084

Submission received: 27 December 2021 / Revised: 25 January 2022 / Accepted: 25 January 2022 / Published: 28 January 2022

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Abstract

Peripheral nerve injuries cause different degrees of nerve palsy and function loss. Due to the limitations of autografts, nerve tissue engineering (TE) scaffolds incorporated with various neurotrophic factors and cells have been investigated to promote nerve regeneration. However, the molecular mechanism is still poorly understood. In this study, we co-cultured Schwann cells (SCs) and rat adrenal pheochromocytoma (PC-12) cells on 50% degrees of methacryloyl substitution gelatin methacrylate (GelMA) scaffold. The SCs were encapsulated within the GelMA, and PC-12 cells were on the surface. A 5% GelMA was used as the co-culture scaffold since it better supports SCs proliferation, viability, and myelination and promotes higher neurotrophic factors secretion than 10% GelMA. In the co-culture, PC-12 cells demonstrated a higher cell proliferation rate and axonal extension than culturing without SCs, indicating that the secretion of neurotrophic factors from SCs can stimulate PC-12 growth and axonal outgrowth. The mRNA level for neurotrophic factors of SCs in 5% GelMA was further evaluated. We found significant upregulation when compared with a 2D culture, which suggested that this co-culture system could be a potential scaffold to investigate the mechanism of how SCs affect neuronal behaviors.

Keywords: gelatin methacrylate; 3D culture; Schwann cells; neurotrophic factors; nerve regeneration

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MDPI and ACS Style

Huang, Y.; Xu, K.; Liu, J.; Dai, G.; Yin, J.; Wei, P. Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate. Gels 2022, 8, 84. https://doi.org/10.3390/gels8020084

AMA Style

Huang Y, Xu K, Liu J, Dai G, Yin J, Wei P. Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate. Gels. 2022; 8(2):84. https://doi.org/10.3390/gels8020084

Chicago/Turabian Style

Huang, Yuye, Kailei Xu, Jingyi Liu, Guangli Dai, Jun Yin, and Peng Wei. 2022. "Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate" Gels 8, no. 2: 84. https://doi.org/10.3390/gels8020084

APA Style

Huang, Y., Xu, K., Liu, J., Dai, G., Yin, J., & Wei, P. (2022). Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate. Gels, 8(2), 84. https://doi.org/10.3390/gels8020084

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Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate

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