Decellularized Periosteum-Derived Hydrogels Promote the Proliferation, Migration and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells
Abstract
:1. Introduction
2. Results
2.1. Characterization of DP
2.2. Turbidimetric Gelation Kinetics
2.3. Rheological Property of dPH
2.4. Characterization of dPH and Matrigel
2.5. Cellular Activities
2.6. Transwell Chemotaxis Assay
2.7. Osteogenic Activities
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Fabrication of the Decellularized Periosteum-Derived Hydrogel (dPH)
5.2. Characterization of the DP
5.3. Turbidimetric Gelation Kinetics
5.4. Rheological Property of dPH
5.5. Morphologies of dPH and Matrigel
5.6. Hydrophilic Properties
5.7. Evaluations of Cellular Activities
5.7.1. Cell Culture
5.7.2. Cellular Morphologies on the Hydrogels
5.7.3. Cell Proliferation
5.7.4. Transwell Chemotaxis Assay
5.7.5. Osteogenic Activity
5.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | Primer Name | Sequences |
---|---|---|
1 | RUNX2 | sense: TACTATGGCACTTCGTCAGGA antisense: GATTCATCCATTCTGCCACTA |
2 | ALP | sense: GGCTGTAAGGACATCGCCTA antisense: GGGTCAAGGGTCAGGAGTTC |
3 | OPN | sense: GCTAAACCCTGACCCATC antisense: CTTTCGTTGGACTTACTTGG |
4 | OCN | sense: AGGGCAGCGAGGTAGTGAAG antisense: CTCCTGAAAGCCGATGTGGT |
5 | GAPDH | sense: GCACCGTCAAGGCTGAGAAC antisense: TGGTGAAGACGCCAGTGGA |
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Li, S.; Deng, R.; Forouzanfar, T.; Wu, G.; Quan, D.; Zhou, M. Decellularized Periosteum-Derived Hydrogels Promote the Proliferation, Migration and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells. Gels 2022, 8, 294. https://doi.org/10.3390/gels8050294
Li S, Deng R, Forouzanfar T, Wu G, Quan D, Zhou M. Decellularized Periosteum-Derived Hydrogels Promote the Proliferation, Migration and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells. Gels. 2022; 8(5):294. https://doi.org/10.3390/gels8050294
Chicago/Turabian StyleLi, Shuyi, Rongli Deng, Tim Forouzanfar, Gang Wu, Daping Quan, and Miao Zhou. 2022. "Decellularized Periosteum-Derived Hydrogels Promote the Proliferation, Migration and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells" Gels 8, no. 5: 294. https://doi.org/10.3390/gels8050294