Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of p(HEMA-co-AGE) Cryogels
2.2. Catalase Binding Studies into p(HEMA-co-ADE) Cryogel from Aqueous Solutions
2.2.1. Effect of Flow Rate
2.2.2. Effects of pH and Initial Catalase Concentration
2.3. Kinetic Parameters
2.4. Effect of Temperature and pH on the Catalytic Activity
2.5. Reusability and Stability of Catalase-Immobilized p(HEMA-co-AGE) Cryogel
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Synthesis and Characterization of p(HEMA-co-AGE) Cryogels
4.3. Catalase Immobilization onto p(HEMA-co-AGE) Cryogels and Desorption of Catalase from p(HEMA-co-AGE) Cryogels
4.4. Activity Assays of Free and Immobilized Catalase Enzymes
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Scanning Area | Bond Type | Energy (eV) |
---|---|---|
A | C-C, C-H | 284.75 |
B | C-O or C-N | 287 |
C | C=O | 288.5 |
D | C-N | 290.5 |
E | COOH | 295 |
p(HEMA) | p(HEMA-co-AGE) |
---|---|
399.08 | 402.64 |
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Altunbaş, C.; Aslan, A.; Kuşat, K.; Sahiner, M.; Akgöl, S.; Sahiner, N. Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase. Gels 2022, 8, 501. https://doi.org/10.3390/gels8080501
Altunbaş C, Aslan A, Kuşat K, Sahiner M, Akgöl S, Sahiner N. Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase. Gels. 2022; 8(8):501. https://doi.org/10.3390/gels8080501
Chicago/Turabian StyleAltunbaş, Canan, Ahmet Aslan, Kevser Kuşat, Mehtap Sahiner, Sinan Akgöl, and Nurettin Sahiner. 2022. "Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase" Gels 8, no. 8: 501. https://doi.org/10.3390/gels8080501