Exploring the Intestinal Microbial Community of Lantang Pigs through Metagenome-Assembled Genomes and Carbohydrate Degradation Genes
, Xiaoyuan Wei 3, Yunxiang Zhao 4, Feilong Deng 1,2, Jiangchao Zhao 3,* and Ying Li 1,2,*Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThis paper is an interesting piece of work.
Can you discuss the degree to which pigs digest fibers, also compared to non-monogastric animals? Which parts of the fibers do they digest? Which feeds are hence more suitable for the Lantang pig than for other pigs?
Can the metagenome be influenced (probiotics)?
How is the metagenome influenced by factors such as antibiotics?
Are there regional differences? The city of your experiments is in southeast China, where only a small part of all pigs are produced (China is divided into 4 key pig-producing regions. These regions are: Yangzi River region (Sichuan, Chongqing city, Guizhou, Hunan, Jiangxi, Jiangsu, Zhejiang and Anhui provinces) with 43.8% of national pork production; Northern China region (Hebei, Shandong and Henan provinces) with 21.6% of national pork production; Northeastern region (Liaoning, Jinlin and Heilongjiang provinces) with 6.3% of national pork production; and Southeastern coast region (Fujian, Guangdong, Yunnan and Hainan) with 13.2% of national pork production (Wang, 2001) https://www.thepigsite.com/articles/china-pork-powerhouse-of-the-world#:~:text=These%20regions%20are%3A%20Yangzi%20River,Northeastern%20region%20(Liaoning%2C%20Jinlin%20and)
Why did you choose 30 and 30 pigs?
Other comments:
46 rimarily acetates, ropionates, and butyrates --> primarily acetate, ropionate, and butyrate
68 predominantly dominated --> word repetition, rephrase
75 fecal sample --> fecal sample
84 A total number of 60 fecal samples -->sentence not complete
175 The phylogenetic tree in Fig. 2 is not readable, use a higher resolution
Author Response
Please find the attachment.Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsThe manuscript is well written and delimited. I suggest some adjustments before acceptance for publication:
I suggest that authors remove words already contained in the text from this item and include new terms. The words contained in the title already serve as terms to search for jobs similar to yours.
Introduction
How does this research differ from previous studies? What is the innovation?
It is necessary to insert a hypothesis in the introduction.
Material and methods
What is the experimental design?
Enter the Ethics Committee protocol number for studies using animals.
More details are needed regarding the animals: What is their average weight and age? How big are the confinement pens? What was the management system? The diet offered was composed of which ingredients? A table with the nutritional composition of the ingredients and the diet offered is necessary. How was this diet formulated? According to which resolution? Did the animals receive water? How was the feces collected? For how many days and during what periods? Was there a total collection of feces and at the end of the collection period were subsamples separated for each animal? Describe in detail.
How were the analysis results handled?
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
