Potential of Endophytic Microorganisms in Fermentative Processes Using Agro-Industrial Waste as Substrates
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fruits
2.2. Agro-Industrial Residues
2.3. Isolation and Selection of Endophytic Microorganisms
2.3.1. Sanitization of Fruits
2.3.2. Isolation of Microorganisms
2.3.3. Selection of Endophytic Microorganisms Using Sensory Analysis
2.4. Identification of the Selected Microorganism
2.5. Fermentation of the Selected Strain with Agro-Industrial Residues
2.6. Analysis of Volatile Compounds
2.7. Statistical Analysis
3. Results and Discussion
3.1. Isolation and Selection of Aroma-Producing Microorganisms
3.2. Identification of the HIM-01 Strain by MALDI-TOF-MS
3.3. Volatile Compounds Obtained from the Fermentation of Kloeckera Apiculata in Culture Medium Supplemented with Agro-Industrial Residues
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Fruits | Number of Yeasts Isolated | Number of Fungi Isolated |
---|---|---|
Strawberry | 10 | 3 |
Lychee | 8 | 2 |
Tangerine | 6 | 8 |
N° | Compounds | IRCal | Area (%) | B.Q. * | B.B. * | Aroma b |
---|---|---|---|---|---|---|
1 | Ethanol | 522 | 10.03 | x | alcoholic | |
2 | Methyl acetate | 560 | 10.72 | x | sweet, fruity | |
3 | 2-Methyl-1-propanol | 550 | 1.81 | x | ||
4 | Ethyl acetate | 640 | 11.14 | x | fruity | |
5 | Isobutyl acetate | 790 | 0.67 | x | fruity | |
6 | Hexanal | 800 | 0.47 | x | green | |
7 | Ethyl butanoate | 802 | 1.74 | x | fruity | |
8 | (Z)-3-Hexen-1-ol | 855 | 1.12 | x | ||
9 | 1-hexanol | 863 | 6.07 | herbs | ||
10 | isoamyl acetate | 877 | 5.95 | x | fruity | |
11 | 2-Methylbutyl acetate | 879 | 1.35 | x | ||
12 | Styrene | 890 | 0.63 | x | ||
13 | 2-Heptanol | 900 | 0.60 | x | citrus | |
14 | Methyl hexanoate | 925 | 0.56 | x | fruity | |
15 | α-Thujene | 934 | 0.54 | x | ||
16 | Benzaldehyde | 960 | 6.33 | x | ||
17 | β-Myrcene | 991 | 3.02 | spicy | ||
18 | β-Cymene | 1019 | 1.12 | x | ||
19 | (E)-Ocimene | 1029 | 0.84 | x | herbs | |
20 | Benzeneacetaldehyde | 1035 | 3.30 | x | green | |
21 | 3-Carene | 1038 | 4.47 | sweet | ||
22 | 4-(benzoylmethyl)-6-methyl-2H-1,4-benzoxazin-3-one | 1058 | 0.56 | x | ||
23 | Terpinolene | 1076 | 0.98 | x | herbs | |
24 | Methyl benzoate | 1082 | 6.51 | x | phenolic | |
25 | 2-Phenylethyl acetate | 1104 | 14.54 | roses | ||
26 | Ethyl benzoate | 1165 | 2.68 | x | mint | |
27 | Hexyl 2-methylpropanoate | 1189 | 2.26 |
N° | Compounds | IRCal | Area (%) | B.Q. * | B.B. * | Aroma b |
---|---|---|---|---|---|---|
1 | Ethanol | 529 | 2.96 | x | x | alcohol |
2 | Ethyl acetate | 644 | 7.89 | x | fruity | |
3 | Isoamyl alcohol | 775 | 1.26 | x | ||
4 | 2-Methyl-1-butanol | 777 | 0.78 | x | ||
5 | Isoamyl acetate | 877 | 4.34 | x | ||
6 | D-limonene | 1022 | 1.21 | x | citric | |
7 | Eucalyptol | 1024 | 0.53 | x | eucalyptus | |
8 | (E)-Ocimene | 1029 | 3.21 | x | ||
9 | 2-Phenylethanol | 1038 | 0.57 | x | floral | |
10 | α-Pyronene | 1109 | 0.55 | |||
11 | 2-Phenylethyl acetate | 1117 | 0.42 | x | floral, roses | |
12 | α-Copaene | 1259 | 0.87 | x | woody | |
13 | β-Caryophyllen | 1374 | 6.26 | x | sweet | |
14 | Aromandendrene | 1419 | 41.23 | x | woody | |
15 | Humulene | 1437 | 7.06 | x | woody | |
16 | Alloaromadendrene | 1451 | 4.55 | xx | woody | |
17 | γ-Muurolene | 1458 | 0.85 | x | herbal | |
18 | β-Selinene | 1474 | 1.22 | x | herbal | |
19 | α-Selinene | 1483 | 6.48 | x | Waxy | |
20 | δ-Cadinene | 1492 | 6.29 | x | herbal | |
21 | (+)-Ledene | 1521 | 0.87 | x | ||
22 | β-Guaiene | 1579 | 0.57 | x | balsamic |
N° | Compounds | IRCal | Area (%) | B.Q. * | B.B. * | Aroma b |
---|---|---|---|---|---|---|
1 | Isoamyl alcohol | 775 | 4.72 | x | fruity | |
2 | 2-Methyl-1-butanol | 776 | 5.10 | x | burned | |
3 | (E)-2-Methylcyclopentanol | 800 | 0.46 | x | ||
4 | 2,4-Dimethylheptane | 821 | 0.89 | x | ||
5 | Furfural | 832 | 37.09 | x | ||
6 | 2-Ethoxy-2-cyclohexenone | 841 | 9.97 | x | ||
7 | 1,3-Dimethylheptane | 863 | 1.67 | x | ||
8 | Isoamyl acetate | 877 | 18.45 | x | fruity | |
9 | 2-Methylbutyl acetate | 880 | 2.49 | x | fruity | |
10 | Methional | 906 | 0.45 | x | ||
11 | 2-Acetylfuran | 913 | 0.42 | x | ||
12 | Benzaldehyde | 961 | 1.89 | x | ||
13 | 2-Pentylfuran | 991 | 0.91 | x | ||
14 | 2-Furanmethanol | 995 | 0.80 | x | ||
15 | Ethyl hexanoate | 999 | 1.60 | fruity | ||
16 | 4-Methyldecane | 1007 | 0.99 | |||
17 | 3-(prop-2-enoyloxy)tetradecane | 1017 | 0.75 | x | ||
18 | Benzeneacetaldehyde | 1035 | 3.46 | x | green | |
19 | 1-Propyldecyl phenylacetate | 1040 | 0.71 | x | ||
20 | 1-Methylodecyl phenylacetate | 1043 | 0.56 | x | ||
21 | 2,6,6-trimethyl-bicyclo[3.1.1]hept-3-ylamine | 1091 | 0.37 | x | ||
22 | Ethyl octanoate | 1195 | 3.90 | wax | ||
23 | 2-Phenylethyl acetate | 1261 | 2.36 |
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Leite Neta, M.T.S.; Sandes, R.D.D.; De Jesus, M.S.; Araujo, H.C.S.; Dos Santos, R.A.R.; Santana, L.C.L.D.A.; Narain, N. Potential of Endophytic Microorganisms in Fermentative Processes Using Agro-Industrial Waste as Substrates. Fermentation 2024, 10, 430. https://doi.org/10.3390/fermentation10080430
Leite Neta MTS, Sandes RDD, De Jesus MS, Araujo HCS, Dos Santos RAR, Santana LCLDA, Narain N. Potential of Endophytic Microorganisms in Fermentative Processes Using Agro-Industrial Waste as Substrates. Fermentation. 2024; 10(8):430. https://doi.org/10.3390/fermentation10080430
Chicago/Turabian StyleLeite Neta, Maria Terezinha Santos, Rafael Donizete Dutra Sandes, Mônica Silva De Jesus, Hannah Caroline Santos Araujo, Raquel Anne Ribeiro Dos Santos, Luciana Cristina Lins De Aquino Santana, and Narendra Narain. 2024. "Potential of Endophytic Microorganisms in Fermentative Processes Using Agro-Industrial Waste as Substrates" Fermentation 10, no. 8: 430. https://doi.org/10.3390/fermentation10080430