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Fermentation
Volume 7
Issue 3
10.3390/fermentation7030146
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Article

Isocitric Acid Production from Ethanol Industry Waste by Yarrowia lipolytica

by
Svetlana V. Kamzolova
*,
Vladimir A. Samoilenko
,
Julia N. Lunina
and
Igor G. Morgunov
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290 Moscow, Russia
*
Author to whom correspondence should be addressed.
Fermentation 2021, 7(3), 146; https://doi.org/10.3390/fermentation7030146
Submission received: 15 July 2021 / Revised: 29 July 2021 / Accepted: 31 July 2021 / Published: 4 August 2021
(This article belongs to the Special Issue Yeast Biotechnology 5.0)
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Abstract

There is ever increasing evidence that isocitric acid can be used as a promising compound with powerful antioxidant activity to combat oxidative stress. This work demonstrates the possibility of using waste product from the alcohol industry (so-called ester-aldehyde fraction) for production of isocitric acid by yeasts. The potential producer of isocitric acid from this fraction, Yarrowia lipolytica VKM Y-2373, was selected by screening of various yeast cultures. The selected strain showed sufficient growth and good acid formation in media with growth-limiting concentrations of nitrogen, sulfur, phosphorus, and magnesium. A shortage of Fe2+ and Ca2+ ions suppressed both Y. lipolytica growth and formation of isocitric acid. The preferential synthesis of isocitric acid can be regulated by changing the nature and concentration of nitrogen source, pH of cultivation medium, and concentration of ester-aldehyde fraction. Experiments in this direction allowed us to obtain 65 g/L isocitric acid with a product yield (YICA) of 0.65 g/g in four days of cultivation.
Keywords: microbial synthesis; yeast; isocitric acid; Yarrowia lipolytica; waste from alcohol industry; ester-aldehyde fraction; optimization microbial synthesis; yeast; isocitric acid; Yarrowia lipolytica; waste from alcohol industry; ester-aldehyde fraction; optimization

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MDPI and ACS Style

Kamzolova, S.V.; Samoilenko, V.A.; Lunina, J.N.; Morgunov, I.G. Isocitric Acid Production from Ethanol Industry Waste by Yarrowia lipolytica. Fermentation 2021, 7, 146. https://doi.org/10.3390/fermentation7030146

AMA Style

Kamzolova SV, Samoilenko VA, Lunina JN, Morgunov IG. Isocitric Acid Production from Ethanol Industry Waste by Yarrowia lipolytica. Fermentation. 2021; 7(3):146. https://doi.org/10.3390/fermentation7030146

Chicago/Turabian Style

Kamzolova, Svetlana V., Vladimir A. Samoilenko, Julia N. Lunina, and Igor G. Morgunov. 2021. "Isocitric Acid Production from Ethanol Industry Waste by Yarrowia lipolytica" Fermentation 7, no. 3: 146. https://doi.org/10.3390/fermentation7030146

APA Style

Kamzolova, S. V., Samoilenko, V. A., Lunina, J. N., & Morgunov, I. G. (2021). Isocitric Acid Production from Ethanol Industry Waste by Yarrowia lipolytica. Fermentation, 7(3), 146. https://doi.org/10.3390/fermentation7030146

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