Transcriptomic Analysis Revealed the Differences in Lipid Accumulation between Spores and Mycelia of Mucor circinelloides WJ11 under Solid–State Fermentation
, Huaiyuan Zhang 1,* and Yuanda Song 1,*Round 1
Reviewer 1 Report
According to Tang X. et al. (2015) Comparison of Biochemical Activities between High and Low Lipid-Producing Strains of Mucor circinelloides: An Explanation for the High Oleaginicity of Strain WJ11. PLoS ONE 10(6): e0128396 M. circinelloides WJ11 are able to produce lipid up to 36% (w/w). It would be interesting to compare the lipid yield per unit medium/substrate during submerged or solid phase fermentation to understand whether this strain is only a model for studying lipid accumulation, or whether it can also be used for the industrial microbial oil production.
Author Response
Comments and Suggestions for Authors：
- According to Tang X. et al. (2015) Comparison of Biochemical Activities between High and Low Lipid-Producing Strains of Mucor circinelloides: An Explanation for the High Oleaginicity of Strain WJ11. PLoS ONE 10(6): e0128396 M. circinelloides WJ11 are able to produce lipid up to 36% (w/w). It would be interesting to compare the lipid yield per unit medium/substrate during submerged or solid phase fermentation to understand whether this strain is only a model for studying lipid accumulation, or whether it can also be used for the industrial microbial oil production.
Response: Thank you very much for your review of this manuscript. Mucor circinelloides WJ11 is a strain retained by our laboratory research group. We have genetically engineered M. circinelloides to increase its oil production so that it can be industrialized. In addition, we will also study the difference of lipid content of mycelium under different fermentation conditions (submerged fermentation and solid-state fermentation).
Reviewer 2 Report
The manuscript entitled Transcriptomic analysis revealed the differences in lipid accumulation between spores and mycelia of Mucor circinelloides WJ11 under solid-state fermentation by Xin et al. describes a comparative analysis of expression data under two cultivation conditions. Overall, the manuscript is well written, and it is easy to follow. However further data availability statements and links are necessary. Author should provide their scripts to perform the RNA-seq analysis.
Additional concern include:
Authors should create a github repository with the expression data.
Authors compare with several photosynthetic and yeast organisms in the results and discussion sections. It would be great if they add a comparative summary table of all findings.
The methods section is missing a figure or picture of how the solid fermentation was established.
In Figure 3 and 4. Add in the caption the color coding of enzymes.
Author Response
Comments and Suggestions for Authors:
- The manuscript entitled Transcriptomic analysis revealed the differences in lipid accumulation between spores and mycelia of Mucor circinelloides WJ11 under solid-state fermentation by Xin et al. describes a comparative analysis of expression data under two cultivation conditions. Overall, the manuscript is well written, and it is easy to follow. However further data availability statements and links are necessary. Author should provide their scripts to perform the RNA-seq analysis.
Response: We are very grateful to you for reading and reviewing this manuscript, and we are very willing to accept your suggestions.
Additional concern include:
- Authors should create a github repository with the expression data.
Response: We are very grateful for your suggestion. We submitted a new repository on the github website and placed the link in lines 510-511 in the manuscript.
- Authors compare with several photosynthetic and yeast organisms in the results and discussion sections. It would be great if they add a comparative summary table of all findings.
Response: Thank you very much for reading and reviewing this manuscript. We added the corresponding table (Table 3) in line 482 of the manuscript.
- The methods section is missing a figure or picture of how the solid fermentation was established.
Response: We are very grateful for your comments. We added the corresponding pictures in the supplementary document (doc.) and added and highlighted line 87 in the manuscript.
- In Figure 3 and 4. Add in the caption the color coding of enzymes.
Response: We are very grateful to the reviewers for their suggestions on this manuscript. We have supplemented and highlighted lines 216-217, 294-295 and 423-424 in the manuscript.
6. English language and style are fine/minor spell check required
Response: We are very grateful to the reviewers for their suggestions. We had revised the manuscript language in several places.
