Effects of Cellulase, Lactobacillus plantarum, and Sucrose on Fermentation Parameters, Chemical Composition, and Bacterial Community of Hybrid Pennisetum Silage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Raw Materials and Silage Preparation
2.2. Chemical Analysis
2.3. Bacterial Community Analysis
2.4. Statistical Analysis
3. Results
3.1. Fermentation Parameters of Hybrid Pennisetum Silage
3.2. Chemical Composition of Hybrid Pennisetum Silage
3.3. Bacterial Community of Hybrid Pennisetum Silage
3.3.1. Bacterial Diversity
3.3.2. Composition of Bacteria
3.3.3. Functions and Ways of Prediction
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Wu, J.; Kamal, N.; Hao, H.; Qian, C.; Liu, Z.; Shao, Y.; Zhong, X.; Xu, B. Endophytic Bacillus megaterium BM18-2 mutated for cadmium accumulation and improving plant growth in Hybrid Pennisetum. Biotechnol. Rep. 2019, 24, e00374. [Google Scholar] [CrossRef] [PubMed]
- Li, J.; Yuan, X.; Desta, S.T.; Dong, Z.; Mugabe, W.; Shao, T. Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak (Bos grunniens) for ensiling Pennisetum sinese. Bioresour. Technol. 2018, 257, 76–83. [Google Scholar] [CrossRef] [PubMed]
- Lianhua, L.; Ying, L.; Yongming, S.; Zhenhong, Y.; Xihui, K.; Yi, Z.; Gaixiu, Y. Effect of bioaugmentation on the microbial community and mono-digestion performance of Pennisetum hybrid. Waste Manag. 2018, 78, 741–749. [Google Scholar] [CrossRef]
- Shah, A.A.; Liu, Z.; Qian, C.; Wu, J.; Sultana, N.; Zhong, X. Potential effect of the microbial fermented feed utilization on physicochemical traits, antioxidant enzyme and trace mineral analysis in rabbit meat. J. Anim. Physiol. Anim. Nutr. 2020, 104, 767–775. [Google Scholar] [CrossRef] [PubMed]
- Li, M.; Zi, X.; Zhou, H.; Hou, G.; Cai, Y. Effects of sucrose, glucose, molasses and cellulase on fermentation quality and in vitro gas production of king grass silage. Anim. Feed. Sci. Technol. 2014, 197, 206–212. [Google Scholar] [CrossRef]
- Zi, X.; Li, M.; Yu, D.; Tang, J.; Zhou, H.; Chen, Y. Natural fermentation quality and bacterial community of 12 Pennisetum sinese varieties in Southern China. Front. Microbiol. 2021, 12, 627820. [Google Scholar] [CrossRef] [PubMed]
- Wu, P.; Li, L.; Jiang, J.; Sun, Y.; Yuan, Z.; Feng, X.; Guo, Y. Effects of fermentative and non-fermentative additives on silage quality and anaerobic digestion performance of Pennisetum purpureum. Bioresour. Technol. 2020, 297, 122425. [Google Scholar] [CrossRef] [PubMed]
- Barbosa, F.C.; Silvello, M.A.; Goldbeck, R. Cellulase and oxidative enzymes: New approaches, challenges and perspectives on cellulose degradation for bioethanol production. Biotechnol. Lett. 2020, 42, 875–884. [Google Scholar] [CrossRef] [PubMed]
- Li, J.; Yuan, X.; Dong, Z.; Mugabe, W.; Shao, T. The effects of fibrolytic enzymes, cellulolytic fungi and bacteria on the fermentation characteristics, structural carbohydrates degradation, and enzymatic conversion yields of Pennisetum sinese silage. Bioresour. Technol. 2018, 264, 123–130. [Google Scholar] [CrossRef] [PubMed]
- Li, M.; Zhou, H.; Zi, X.; Cai, Y. Silage fermentation and ruminal degradation of stylo prepared with lactic acid bacteria and cellulase. Anim. Sci. J. 2017, 88, 1531–1537. [Google Scholar] [CrossRef] [PubMed]
- Ren, H.; Feng, Y.; Pei, J.; Li, J.; Wang, Z.; Fu, S.; Zheng, Y.; Li, Z.; Peng, Z. Effects of Lactobacillus plantarum additive and temperature on the ensiling quality and microbial community dynamics of cauliflower leaf silages. Bioresour. Technol. 2020, 307, 123238. [Google Scholar] [CrossRef] [PubMed]
- Guo, X.S.; Ke, W.C.; Ding, W.R.; Ding, L.M.; Xu, D.M.; Wang, W.W.; Zhang, P.; Yang, F.Y. Profiling of metabolome and bacterial community dynamics in ensiled Medicago sativa inoculated without or with Lactobacillus plantarum or Lactobacillus buchneri. Sci. Rep. 2018, 8, 357. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Xu, D.; Ding, Z.; Bai, J.; Ke, W.; Zhang, Y.; Li, F.; Guo, X. Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp. Bioresour. Technol. 2020, 310, 123476. [Google Scholar] [CrossRef] [PubMed]
- Shao, T.; Shimojo, M.; Wang, T.; Masuda, Y. Effect of additives on the fermentation quality and residual mono-and disaccharides compositions of forage oats (Avena sativa L.) and Italian ryegrass (Lolium multiflorum Lam.) silages. Asian-Australas. J. Anim. Sci. 2005, 18, 1582–1588. [Google Scholar] [CrossRef]
- Madrid, J.; Martínez-Teruel, A.; Hernández, F.; Megías, M.D. A comparative study on the determination of lactic acid in silage juice by colorimetric, high-performance liquid chromatography and enzymatic methods. J. Sci. Food Agric. 1999, 79, 1722–1726. [Google Scholar] [CrossRef]
- Ke, W.; Ding, W.; Xu, D.; Ding, L.; Zhang, P.; Li, F.; Guo, X. Effects of addition of malic or citric acids on fermentation quality and chemical characteristics of alfalfa silage. J. Dairy Sci. 2017, 100, 8958–8966. [Google Scholar] [CrossRef] [Green Version]
- Association of Official Analytical Chemists. Official methods of analysis, 15th ed.; Association of Official Analytical Chemists: Arlington, VA, USA, 1990. [Google Scholar]
- Van Soest, P.V.; Robertson, J.B.; Lewis, B.A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 1991, 74, 3583–3597. [Google Scholar] [CrossRef]
- Douglas, G.M.; Beiko, R.G.; Langille, M.G. Predicting the functional potential of the microbiome from marker genes using PICRUSt. In Microbiome Analysis; Humana Press: New York, NY, USA, 2018; pp. 169–177. [Google Scholar] [CrossRef]
- Li, L.; Yuan, Z.; Sun, Y.; Kong, X.; Dong, P.; Zhang, J. A reused method for molasses-processed wastewater: Effect on silage quality and anaerobic digestion performance of Pennisetum purpereum. Bioresour. Technol. 2017, 241, 1003–1011. [Google Scholar] [CrossRef]
- Weinberg, Z.; Muck, R.; Weimer, P. The survival of silage inoculant lactic acid bacteria in rumen fluid. J. Appl. Microbiol. 2003, 94, 1066–1071. [Google Scholar] [CrossRef] [Green Version]
- Li, F.; Ke, W.; Ding, Z.; Bai, J.; Zhang, Y.; Xu, D.; Li, Z.; Guo, X. Pretreatment of Pennisetum sinese silages with ferulic acid esterase-producing lactic acid bacteria and cellulase at two dry matter contents: Fermentation characteristics, carbohydrates composition and enzymatic saccharification. Bioresour. Technol. 2020, 295, 122261. [Google Scholar] [CrossRef]
- Contreras-Govea, F.E.; Muck, R.E.; Broderick, G.A.; Weimer, P.J. Lactobacillus plantarum effects on silage fermentation and in vitro microbial yield. Anim. Feed. Sci. Technol. 2013, 179, 61–68. [Google Scholar] [CrossRef]
- McDonald, P.; Henderson, N.; Heron, S. The Biochemistry of Silage; Cambridge University Press: Cambridge, UK, 1991. [Google Scholar]
- Parvin, S.; Wang, C.; Li, Y.; Nishino, N. Effects of inoculation with lactic acid bacteria on the bacterial communities of Italian ryegrass, whole crop maize, guinea grass and rhodes grass silages. Anim. Feed. Sci. Technol. 2010, 160, 160–166. [Google Scholar] [CrossRef]
- Bai, J.; Xu, D.; Xie, D.; Wang, M.; Li, Z.; Guo, X. Effects of antibacterial peptide-producing Bacillus subtilis and Lactobacillus buchneri on fermentation, aerobic stability, and microbial community of alfalfa silage. Bioresour. Technol. 2020, 315, 123881. [Google Scholar] [CrossRef] [PubMed]
- He, L.; Wang, C.; Xing, Y.; Zhou, W.; Pian, R.; Chen, X.; Zhang, Q. Ensiling characteristics, proteolysis and bacterial community of high-moisture corn stalk and stylo silage prepared with Bauhinia variegate flower. Bioresour. Technol. 2020, 296, 122336. [Google Scholar] [CrossRef]
- Vicente, F.; Rodríguez, M.L.; Martínez-Fernández, A.; Soldado, A.; Argamentería, A.; Peláez, M.; de la Roza-Delgado, B. Subclinical ketosis on dairy cows in transition period in farms with contrasting butyric acid contents in silages. Sci. World J. 2014, 2014, 279614. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Desta, S.T.; Yuan, X.; Li, J.; Shao, T. Ensiling characteristics, structural and nonstructural carbohydrate composition and enzymatic digestibility of Napier grass ensiled with additives. Bioresour. Technol. 2016, 221, 447–454. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Pahlow, G.; Muck, R.E.; Driehuis, F.; Elferink, S.J.O.; Spoelstra, S.F. Microbiology of ensiling. Silage Sci. Technol. 2003, 42, 31–93. [Google Scholar] [CrossRef]
- Khota, W.; Pholsen, S.; Higgs, D.; Cai, Y. Natural lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant. J. Dairy Sci. 2016, 99, 9768–9781. [Google Scholar] [CrossRef] [Green Version]
- Liu, B.; Yang, Z.; Huan, H.; Gu, H.; Xu, N.; Ding, C. Impact of molasses and microbial inoculants on fermentation quality, aerobic stability, and bacterial and fungal microbiomes of barley silage. Sci. Rep. 2020, 10, 5342. [Google Scholar] [CrossRef] [Green Version]
- Mu, L.; Xie, Z.; Hu, L.; Chen, G.; Zhang, Z. Cellulase interacts with Lactobacillus plantarum to affect chemical composition, bacterial communities, and aerobic stability in mixed silage of high-moisture amaranth and rice straw. Bioresour. Technol. 2020, 315, 123772. [Google Scholar] [CrossRef]
- Chi, Z.; Deng, M.; Tian, H.; Liu, D.; Li, Y.; Liu, G.; Sun, B.; Guo, Y. Effects of Mulberry Leaves and Pennisetum Hybrid Mix-Silage on Fermentation Parameters and Bacterial Community. Fermentation 2022, 8, 197. [Google Scholar] [CrossRef]
- Yuan, X.; Li, J.; Dong, Z.; Shao, T. The reconstitution mechanism of napier grass microiota during the ensiling of alfalfa and their contributions to fermentation quality of silage. Bioresour. Technol. 2020, 297, 122391. [Google Scholar] [CrossRef] [PubMed]
- Yuan, X.; Dong, Z.; Li, J.; Shao, T. Microbial community dynamics and their contributions to organic acid production during the early stage of the ensiling of Napier grass (Pennisetum purpureum). Grass Forage Sci. 2019, 75, 37–44. [Google Scholar] [CrossRef]
- Wang, M.; Wang, L.; Yu, Z. Fermentation dynamics and bacterial diversity of mixed lucerne and sweet corn stalk silage ensiled at six ratios. Grass Forage Sci. 2019, 74, 264–273. [Google Scholar] [CrossRef]
- Scheirlinck, T.; De Meutter, J.; Arnaut, G.; Joos, H.; Claeyssens, M.; Michiels, F. Cloning and expression of cellulase and xylanase genes in Lactobacillus plantarum. Appl. Microbiol. Biotechnol. 1990, 33, 534–541. [Google Scholar] [CrossRef]
- Graf, K.; Ulrich, A.; Idler, C.; Klocke, M. Bacterial community dynamics during ensiling of perennial ryegrass at two compaction levels monitored by terminal restriction fragment length polymorphism. J. Appl. Microbiol. 2016, 120, 1479–1491. [Google Scholar] [CrossRef] [Green Version]
- Wali, A.; Nishino, N. Bacterial and fungal microbiota associated with the ensiling of wet soybean curd residue under prompt and delayed sealing conditions. Microorganisms 2020, 8, 1334. [Google Scholar] [CrossRef] [PubMed]
- Sun, R.; Yuan, X.; Li, J.; Tao, X.; Dong, Z.; Shao, T. Contributions of epiphytic microbiota on the fermentation characteristics and microbial composition of ensiled six whole crop corn varieties. J. Appl. Microbiol. 2021, 131, 1683–1694. [Google Scholar] [CrossRef] [PubMed]
- Hao, W.; Tian, P.; Zheng, M.; Wang, H.; Xu, C. Characteristics of proteolytic microorganisms and their effects on proteolysis in total mixed ration silages of soybean curd residue. Asian-Australas. J. Anim. Sci. 2020, 33, 100. [Google Scholar] [CrossRef] [Green Version]
- Sun, L.; Bai, C.; Xu, H.; Na, N.; Jiang, Y.; Yin, G.; Liu, S.; Xue, Y. Succession of bacterial community during the initial aerobic, intense fermentation, and stable phases of whole-plant corn silages treated with lactic acid bacteria suspensions prepared from other silages. Front. Microbiol. 2021, 12, 591. [Google Scholar] [CrossRef]
- Trček, J.; Mira, N.P.; Jarboe, L.R. Adaptation and tolerance of bacteria against acetic acid. Appl. Microbiol. Biotechnol. 2015, 99, 6215–6229. [Google Scholar] [CrossRef] [PubMed]
Items | Treatments | SEM | p-Value | ||||||
---|---|---|---|---|---|---|---|---|---|
CON | CE | LP | SU | CE+LP | CE+SU | LP+SU | |||
pH | 3.70 ab | 3.61 c | 3.60 c | 3.68 b | 3.71 a | 3.71 a | 3.72 a | 0.01 | <0.001 |
LA (g/kg DM) | 20.62 | 22.70 | 21.34 | 22.13 | 16.51 | 19.12 | 17.85 | 2.51 | 0.199 |
AA (g/kg DM) | 9.58 c | 9.78 bc | 9.62 c | 10.36 bc | 14.58 a | 13.99 a | 11.37 b | 0.79 | <0.001 |
WSC (g/kg DM) | 8.50 c | 11.34 ab | 9.64 bc | 11.02 ab | 9.90 bc | 9.88 bc | 12.33 a | 0.94 | 0.021 |
AN (g/kg DM) | 0.87 a | 0.69 b | 0.62 b | 0.62 b | 0.66 b | 0.66 b | 0.72 b | 0.07 | 0.031 |
BA (g/kg DM) | — | — | — | 0.47 | — | — | — | — | — |
Items | Treatments | SEM | p-Value | ||||||
---|---|---|---|---|---|---|---|---|---|
CON | CE | LP | SU | CE+LP | CE+SU | LP+SU | |||
DM (g/kg FM) | 324.88 a | 298.07 d | 305.24 cd | 323.45 a | 308.29 bc | 308.73 bc | 315.26 b | 3.42 | <0.001 |
NDF (g/kg DM) | 625.31 a | 547.63 d | 588.99 bc | 607.22 ab | 585.59 bc | 565.00 cd | 614.48 ab | 14.93 | 0.002 |
ADF (g/kg DM) | 339.88 a | 288.13 d | 307.56 bd | 318.45 ab | 313.78 bc | 291.92 cd | 330.05 ab | 11.60 | 0.012 |
CP (g/kg DM) | 55.66 c | 82.32 b | 57.64 c | 56.29 c | 78.01 b | 87.54 a | 48.30 d | 2.08 | <0.001 |
Items | Treatments | SEM | p-Value | ||||||
---|---|---|---|---|---|---|---|---|---|
CON | CE | LP | SU | CE+LP | CE+SU | LP+SU | |||
Good’s coverage | 0.996 a | 0.995 a | 0.995 a | 0.995 a | 0.995 a | 0.995 ab | 0.992 b | 0.001 | 0.200 |
Observed species | 368.53 b | 370.60 b | 412.53 b | 354.37 b | 400.40 b | 402.13 b | 606.10 a | 61.91 | 0.017 |
Shannon index | 4.19 ab | 4.34 ab | 3.83 bc | 3.88 bc | 3.48 c | 4.07 b | 4.63 a | 0.25 | 0.009 |
Simpson index | 0.81 ab | 0.85 a | 0.79 ab | 0.78 bc | 0.71 c | 0.81 ab | 0.82 ab | 0.03 | 0.017 |
Chao1 index | 462.06 b | 478.40 b | 517.75 b | 457.59 b | 503.57 b | 515.10 b | 765.93 a | 97.49 | 0.080 |
Pielou evenness | 0.49 ab | 0.51 a | 0.44 bc | 0.46 ab | 0.40 c | 0.47 ab | 0.50 a | 0.02 | 0.007 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Xiong, H.; Zhu, Y.; Wen, Z.; Liu, G.; Guo, Y.; Sun, B. Effects of Cellulase, Lactobacillus plantarum, and Sucrose on Fermentation Parameters, Chemical Composition, and Bacterial Community of Hybrid Pennisetum Silage. Fermentation 2022, 8, 356. https://doi.org/10.3390/fermentation8080356
Xiong H, Zhu Y, Wen Z, Liu G, Guo Y, Sun B. Effects of Cellulase, Lactobacillus plantarum, and Sucrose on Fermentation Parameters, Chemical Composition, and Bacterial Community of Hybrid Pennisetum Silage. Fermentation. 2022; 8(8):356. https://doi.org/10.3390/fermentation8080356
Chicago/Turabian StyleXiong, Haoming, Yanchen Zhu, Zhiying Wen, Guangbin Liu, Yongqing Guo, and Baoli Sun. 2022. "Effects of Cellulase, Lactobacillus plantarum, and Sucrose on Fermentation Parameters, Chemical Composition, and Bacterial Community of Hybrid Pennisetum Silage" Fermentation 8, no. 8: 356. https://doi.org/10.3390/fermentation8080356