Enzymatic One-Pot Hydrolysis of Extracted Sugar Beet Press Pulp after Solid-State Fermentation with an Engineered Aspergillus niger Strain
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strain
2.1.1. Cloning of the A. niger gaaX Deletion Construct
2.1.2. Genetic Manipulation of A. niger
2.2. Sugar Beet Press Pulp
2.3. Sugar Beet Press Pulp Composition Analysis
2.4. Preculture, Inoculum Preparation, and Cultivation Media
2.5. Solid-State Fermentation
2.6. Subsequent Hydrolysis of Sugar Beet Press Pulp
2.7. Quantification of Sugars and Organic Acids in Hydrolysis Supernatants
2.8. Gravimetric Biomass Analysis of Hydrolysis Samples
2.9. Total Polygalacturonase Activity Assay
3. Results and Discussion
3.1. Composition of SBPP
3.2. Integrated Process for Enzyme Production and Subsequent Hydrolysis
3.3. Solid-State Fermentation with Engineered A. niger
3.4. Subsequent Hydrolysis of SBPP
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SBPP Fraction | Particle Size x, mm | Fractional Share, % | Standard Deviation, % |
---|---|---|---|
Non-milled | 4.0 < x < 6.3 2.0 < x < 4.0 0.5 < x < 2.0 | 10.48 62.76 26.76 | 3.71 2.43 1.74 |
Milled | 1.0 < x < 2.0 0.5 < x < 1.0 x < 0.5 | 8.28 57.22 34.55 | 9.61 5.52 11.22 |
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Knesebeck, M.; Schäfer, D.; Schmitz, K.; Rüllke, M.; Benz, J.P.; Weuster-Botz, D. Enzymatic One-Pot Hydrolysis of Extracted Sugar Beet Press Pulp after Solid-State Fermentation with an Engineered Aspergillus niger Strain. Fermentation 2023, 9, 582. https://doi.org/10.3390/fermentation9070582
Knesebeck M, Schäfer D, Schmitz K, Rüllke M, Benz JP, Weuster-Botz D. Enzymatic One-Pot Hydrolysis of Extracted Sugar Beet Press Pulp after Solid-State Fermentation with an Engineered Aspergillus niger Strain. Fermentation. 2023; 9(7):582. https://doi.org/10.3390/fermentation9070582
Chicago/Turabian StyleKnesebeck, Melanie, Dominik Schäfer, Kevin Schmitz, Marcel Rüllke, J. Philipp Benz, and Dirk Weuster-Botz. 2023. "Enzymatic One-Pot Hydrolysis of Extracted Sugar Beet Press Pulp after Solid-State Fermentation with an Engineered Aspergillus niger Strain" Fermentation 9, no. 7: 582. https://doi.org/10.3390/fermentation9070582