A MYB Transcription Factor from Brassica juncea Regulates Purple Leaves in Pak Choi (Brassica campestris L. ssp. chinensis)

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The submitted manuscript titled “A MYB transcription factor from Brassica juncea regulates the purple leaf in pak-choi (Brassica campestris ssp. chinensis)” is an intriguing review article. In terms of thematic relevance, it aligns well with the scientific scope of the journal  Horticulturae.

However, there are several areas where the quality of the manuscript can be enhanced:

1.      Abstract: Provide a concise summary of the research findings and critically evaluate the obtained results.

2.      Introduction: Conclude this section by clearly stating the research objectives and presenting the research hypotheses.

3.      Text Formatting: Correct formatting issues, such as removing lines 380-387 and 516-517.

4.      Conclusion: Elaborate further on the obtained results. Discuss their practical implications and potential applications.

5.      References: Improve the consistency in citation format. Ensure uniformity in abbreviations and full names of journals.

6.      Literature Review: Revisit the literature, as a significant portion (55%) appears to be older than the last decade. Incorporate more recent research to enhance the scientific value of the article.

 

7.      Reference List: Verify that all cited references in the manuscript are included in the reference list, maintaining consistent spelling and publication dates.

Author Response

Point-by-point response to reviewer 1

Dear reviewer:

We sincerely appreciate your consideration of our manuscript. We have amended the relevant parts of the manuscript according to your advice and the reviewers’ suggestions.

The feedback was insightful and enabled us to improve the quality of our manuscript. We have provided point-by-point responses to each of the reviewers’ comments, below. We sincerely hope that the revisions in the manuscript and our accompanying responses meet the requirements for publication in Horticulturae.

We look forward to hearing from you at your earliest convenience.

Yours sincerely,

 

 

Comment 1:  Abstract: Provide a concise summary of the research findings and critically evaluate the obtained results.

Response: We have removed the redundant content of the abstract, and summarized and objectively evaluated the research results. And ABSTRACT was revised in lines 15-16, 17-18, 25-28.

 

Comment 2:  Introduction: Conclude this section by clearly stating the research objectives and presenting the research hypotheses.

Response: We re-examined the introduction, summarized the research objectives and proposed the research hypothesis. This was changed in lines 124-127.

 

Comment 3: Text Formatting: Correct formatting issues, such as removing lines 380-387 and 516-517.

Response: We have rechecked the manuscript, and the formatting issues have been corrected.

 

Comment 4: Conclusion: Elaborate further on the obtained results. Discuss their practical implications and potential applications.

Response: Based on the comment, we revised the whole manuscript, summarize the experimental results and further discuss their practical significance and potential value. And added CONCLUSION in lines 486-488 and 491-494.

 

Comment 5: References: Improve the consistency in citation format. Ensure uniformity in abbreviations and full names of journals.

Response: All references have been reviewed, and the full name of the journal name is selected. This was changed in lines 510-639.

 

Comment 6: Literature Review: Revisit the literature, as a significant portion (55%) appears to be older than the last decade. Incorporate more recent research to enhance the scientific value of the article.

Response: We re-included more recent research and revised some references to improve the rigor and scientific value of the article. These were changed in lines 48-49, 51, 118-119, 513-514, 517-519, 522-533, 538-540, 550-556, 587-588, 600-601.

 

Comment 7: Reference List: Verify that all cited references in the manuscript are included in the reference list, maintaining consistent spelling and publication dates.

Response: We have reviewed all references cited in the manuscript for consistency with the content and checked spelling and publication dates for accuracy.

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The manuscript “A MYB transcription factor from Brassica juncea regulates the purple leaf in pak-choi (Brassica campestris ssp. chinensis)” is dealing with anthocyanin accumulation in purple leaves of commercially available Brassica spp. The purple vegetables have high economic values since they are rich in anthocyanins and are one of the main goals for breeders. Authors evidenced thet the purple-leaf phenotype of the pak-choi varieties derived from purple B. juncea through distant hybridization. Presented results shown that a MYB transcription factors from Brassica juncea regulates the purple leaf in pak-choi. Furthermore, The results of presented study provide a good base for identifying the introgression breeding of Brassica and revealed the recombination of genes in different genomes in Brassica crops. The results has some merit and belongs to journal Horticulture. The manuscript can be accepted for publication after minor corrections that are highlighted in text and described to authors. The errors are mainly typocogical.

Comments for author File: Comments.pdf

Author Response

Point-by-point response to reviewer 2

 

Dear reviewer:

We sincerely appreciate your consideration of our manuscript. We have amended the relevant parts of the manuscript according to your advice and the reviewers’ suggestions.

The feedback was insightful and enabled us to improve the quality of our manuscript. We have provided point-by-point responses to each of the reviewers’ comments, below. We sincerely hope that the revisions in the manuscript and our accompanying responses meet the requirements for publication in Horticulturae.

We look forward to hearing from you at your earliest convenience.

Yours sincerely,

 

Comment:   The manuscript “A MYB transcription factor from Brassica juncea regulates the purple leaf in pak-choi (Brassica campestris ssp. chinensis)” is dealing with anthocyanin accumulation in purple leaves of commercially available Brassica spp. The purple vegetables have high economic values since they are rich in anthocyanins and are one of the main goals for breeders. Authors evidenced thet the purple-leaf phenotype of the pak-choi varieties derived from purple B. juncea through distant hybridization. Presented results shown that a MYB transcription factors from Brassica juncea regulates the purple leaf in pak-choi. Furthermore, The results of presented study provide a good base for identifying the introgression breeding of Brassica and revealed the recombination of genes in different genomes in Brassica crops. The results has some merit and belongs to journal Horticulture. The manuscript can be accepted for publication after minor corrections that are highlighted in text and described to authors. The errors are mainly typocogical.

 

Response: Thanks for your careful checks. We are sorry for our carelessness. Based on your comments, we have made the corrections to make the word harmonized within the whole manuscript. These were changed in lines 1, 236, 285, 302, 306, 310, 327, 394 and 433.

 

Please see the attachment.

Author Response File: Author Response.pdf