Comprehensive Analysis of Microbiomes and Metabolomics Reveals the Mechanism of Adaptation to Cadmium Stress in Rhizosphere Soil of Rhododendron decorum subsp. Diaprepes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experiments on Plant Growth and Cd Addition
2.2. Analysis of Rhizosphere Soil’s Physical Physicochemical Properties
2.3. DNA Extraction, Amplicon Sequencing, and Bioinformatics Analysis
2.4. Rhizosphere Soil Metabolomics and Data Analysis
2.5. Statistical Analysis
3. Results
3.1. The Physicochemical Properties of Rhizosphere Soil at Different CdCl2 Concentrations
3.2. Effects of CdCl2 on Diversity and Composition of Rhizosphere Soil Bacterial and Fungal Communities
3.3. Measurement of Metabolites in Rhizosphere Soil Treated with CdCl2
3.4. Correlations among Microbial Communities, Physicochemical Properties, and Metabolites in Rhizosphere Soil
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Total Edges | Positive Edges | Negative Edges | Connectance | Average Degree | Average Pathway Length | Diameter | Clustering Coefficient | ||
---|---|---|---|---|---|---|---|---|---|
Bac | CK vs. 2.5 mM | 630 | 339 | 291 | 0.49 | 24.7 | 1.31 | 4.79 | 0.87 |
CK vs. 5 mM | 569 | 377 | 192 | 0.45 | 22.3 | 1.33 | 4.20 | 0.90 | |
Fun | CK vs. 2.5 mM | 558 | 305 | 253 | 0.54 | 24.3 | 0.96 | 2.88 | 0.90 |
CK vs. 5 mM | 533 | 350 | 183 | 0.49 | 22.7 | 1.15 | 2.87 | 0.89 |
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Tang, M.; Chen, L.; Wang, L.; Yi, Y.; Wang, J.; Wang, C.; Chen, X.; Liu, J.; Yang, Y.; Malik, K.; et al. Comprehensive Analysis of Microbiomes and Metabolomics Reveals the Mechanism of Adaptation to Cadmium Stress in Rhizosphere Soil of Rhododendron decorum subsp. Diaprepes. Horticulturae 2024, 10, 884. https://doi.org/10.3390/horticulturae10080884
Tang M, Chen L, Wang L, Yi Y, Wang J, Wang C, Chen X, Liu J, Yang Y, Malik K, et al. Comprehensive Analysis of Microbiomes and Metabolomics Reveals the Mechanism of Adaptation to Cadmium Stress in Rhizosphere Soil of Rhododendron decorum subsp. Diaprepes. Horticulturae. 2024; 10(8):884. https://doi.org/10.3390/horticulturae10080884
Chicago/Turabian StyleTang, Ming, Lanlan Chen, Li Wang, Yin Yi, Jianfeng Wang, Chao Wang, Xianlei Chen, Jie Liu, Yongsong Yang, Kamran Malik, and et al. 2024. "Comprehensive Analysis of Microbiomes and Metabolomics Reveals the Mechanism of Adaptation to Cadmium Stress in Rhizosphere Soil of Rhododendron decorum subsp. Diaprepes" Horticulturae 10, no. 8: 884. https://doi.org/10.3390/horticulturae10080884