PhSPEAR1 Participates in Regulating the Branch Development of Petunia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Isolation of PhSPEAR1
2.3. Bioinformatic Analysis
2.4. Subcellular Localization Analysis
2.5. qRT-PCR Analysis
2.6. Construction and Transformation of 35S::PhSPEAR1
2.7. Arabidopsis Transformation
2.8. Phenotype Analysis and Statistical Analysis
2.9. Sample Preparation and Plant Hormone Extraction
2.10. Statistical Analysis
3. Results
3.1. Identification of SPEAR1 in Petunia
3.2. Tissue-Specific Expression Analysis of PhSPEAR1
3.3. Expression Analysis of PhSPEAR1 under Branch-Inducing Treatments
3.4. Subcellular Localization Analysis of PhSPEAR1
3.5. Overexpression of PhSPEAR1 Increases Branch Number in Arabidopsis
3.6. PhSPEAR1 Regulates the Synthesis of CKs, IAA, and ABA
4. Discussion
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Wu, W.; Li, C.; Zhang, Q.; Wang, Y.; Li, X.; Yang, Q.; Dong, L. PhSPEAR1 Participates in Regulating the Branch Development of Petunia. Horticulturae 2024, 10, 912. https://doi.org/10.3390/horticulturae10090912
Wu W, Li C, Zhang Q, Wang Y, Li X, Yang Q, Dong L. PhSPEAR1 Participates in Regulating the Branch Development of Petunia. Horticulturae. 2024; 10(9):912. https://doi.org/10.3390/horticulturae10090912
Chicago/Turabian StyleWu, Wei, Chaoqun Li, Qi Zhang, Yan Wang, Xueying Li, Qin Yang, and Lili Dong. 2024. "PhSPEAR1 Participates in Regulating the Branch Development of Petunia" Horticulturae 10, no. 9: 912. https://doi.org/10.3390/horticulturae10090912