Genome-Wide Identification of DREB Transcription Factor Family and Functional Analysis of PaDREB1D Associated with Low-Temperature Stress in Phalaenopsis aphrodite
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Stress Treatments
2.2. Total RNA Extraction and Real-Time Quantitative PCR (RT-qPCR)
2.3. Screening of DREB Family Members in Phalaenopsis aphrodite
2.4. Phylogenetic Tree Construction and Gene Structure Analysis
2.5. Expression Pattern Analysis of PaDREBs in Different Tissues
2.6. Gene Cloning and Subcellular Localization
2.7. Preparation and Detection of Transgenic Phalaenopsis
2.8. Cold Tolerance Assays of PLBs
2.9. Physiological Measurements
2.10. Statistical Analysis
3. Results
3.1. Identification of DREB Family Members in P. aphrodite and Phylogenetic Tree Construction
3.2. Analysis of the PaDREB Expression Patterns in Different Tissues
3.3. PaDREB1D Responded to Four Treatments and Was Expressed in Different Tissues
3.4. Gene Isolation and Molecular Characterization Analysis of PaDREB1D
3.5. Subcellular Localization of PaDREB1D
3.6. PaDREB1D Overexpression Enhanced Cold Tolerance in PLBs
3.7. Overexpression of the PaDREB1D Gene Reduced Cellular Damage to PLBs
3.8. PaDREB1D Overexpression Enhanced the Antioxidant Enzyme Activities of PLBs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Hu, Z.; Wang, S.; Wang, Y.; Li, J.; Luo, P.; Xin, J.; Cui, Y. Genome-Wide Identification of DREB Transcription Factor Family and Functional Analysis of PaDREB1D Associated with Low-Temperature Stress in Phalaenopsis aphrodite. Horticulturae 2024, 10, 933. https://doi.org/10.3390/horticulturae10090933
Hu Z, Wang S, Wang Y, Li J, Luo P, Xin J, Cui Y. Genome-Wide Identification of DREB Transcription Factor Family and Functional Analysis of PaDREB1D Associated with Low-Temperature Stress in Phalaenopsis aphrodite. Horticulturae. 2024; 10(9):933. https://doi.org/10.3390/horticulturae10090933
Chicago/Turabian StyleHu, Ziang, Shuang Wang, Yaoling Wang, Jiaming Li, Ping Luo, Jingjing Xin, and Yongyi Cui. 2024. "Genome-Wide Identification of DREB Transcription Factor Family and Functional Analysis of PaDREB1D Associated with Low-Temperature Stress in Phalaenopsis aphrodite" Horticulturae 10, no. 9: 933. https://doi.org/10.3390/horticulturae10090933