Plant Extract Treatments Induce Resistance to Bacterial Spot by Tomato Plants for a Sustainable System
Abstract
:1. Introduction
2. Materials and Methods
2.1. Seeds, Growth of Seedlings and Bacterial Isolates
2.2. Preparation of Leaf Extracts
2.3. Effect of Plant Extracts on Pathogen Growth In Vitro
2.4. Effect of Plant Extracts on Disease Severity and Pathogen Population
2.4.1. Preparation of Inoculum and Inoculation Methods
2.4.2. Determination of Pathogen Population on Tomato Leaves
2.4.3. Determination of Fresh and Dry Weight
2.5. Determination of Total Phenols and Salicylic Acid Contents
2.5.1. Preparation of Samples
2.5.2. Total Phenol Content
2.5.3. Salicylic Acid Content
2.6. Enzymatic Activities
2.6.1. Peroxidase Activity (PO)
2.6.2. Polyphenol Oxidase (PPO) Activity
2.7. Statistical Analysis
3. Results and Discussion
3.1. Effect of Plant Extracts on Pathogen Growth In Vitro
3.2. Determination of Pathogen Population on the Tomato Leaves
3.3. Effect of Plant Extracts on Disease Severity and Dry Weight of Shoots
3.4. Effect of Plant Extracts on Total Phenol and Salicylic Acid Contents
3.5. Effect of Plant Extracts on Peroxidase (PO)
3.6. Effect of Plant Extract on Polyphenol Oxidase (PPO)
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Plant Extracts | Method of Extract | CFU/g z |
---|---|---|
Nerium oleander | Water extract | 6.2 ± 0.15 b y |
Ethanol extract | 5.0 ± 0.72 c | |
Eucalyptus chamadulonsis | Water extract | 7.7 ± 0.38 b |
Ethanol extract | 5.1 ± 0.23 c | |
Citrullus colocynthis | Water extract | 4.0 ± 0.15 d |
Ethanol extract | 3.0 ± 0.45 d | |
Controls | Infected | 9.7 ± 0.53 a |
Healthy | 0 e |
Plant Extracts | Method of Extract | Disease Severity (%) | Shoot Weight (gm) |
---|---|---|---|
Nerium oleander | Water extract | 29.13 ± 0.10 z c y | 25.2 ± 0.30 c |
Ethanol extract | 30.00 ± 1.51 c | 25.5 ± 0.38 c | |
Eucalyptus chamadulonsis | Water extract | 39.90 ± 0.68 b | 26.8 ± 0.60 c |
Ethanol extract | 33.13 ± 0.10 cd | 24.2 ± 0.30 c | |
Citrullus colocynthis | Water extract | 21.23 ± 0.17 e | 43.0 ± 1.51 ab |
Ethanol extract | 20.00 ± 0.76 e | 44.7 ± 0.45 ab | |
Controls | Infected | 45.23 ± 0.98 a | 13.2 ± 0.30 d |
Healthy | 0 f | 45.2 ± 0.91 a |
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Abo-Elyousr, K.A.M.; Almasoudi, N.M.; Abdelmagid, A.W.M.; Roberto, S.R.; Youssef, K. Plant Extract Treatments Induce Resistance to Bacterial Spot by Tomato Plants for a Sustainable System. Horticulturae 2020, 6, 36. https://doi.org/10.3390/horticulturae6020036
Abo-Elyousr KAM, Almasoudi NM, Abdelmagid AWM, Roberto SR, Youssef K. Plant Extract Treatments Induce Resistance to Bacterial Spot by Tomato Plants for a Sustainable System. Horticulturae. 2020; 6(2):36. https://doi.org/10.3390/horticulturae6020036
Chicago/Turabian StyleAbo-Elyousr, Kamal A. M., Najeeb M. Almasoudi, Ahmed W. M. Abdelmagid, Sergio R. Roberto, and Khamis Youssef. 2020. "Plant Extract Treatments Induce Resistance to Bacterial Spot by Tomato Plants for a Sustainable System" Horticulturae 6, no. 2: 36. https://doi.org/10.3390/horticulturae6020036
APA StyleAbo-Elyousr, K. A. M., Almasoudi, N. M., Abdelmagid, A. W. M., Roberto, S. R., & Youssef, K. (2020). Plant Extract Treatments Induce Resistance to Bacterial Spot by Tomato Plants for a Sustainable System. Horticulturae, 6(2), 36. https://doi.org/10.3390/horticulturae6020036