Optimum Discrimination on the Subsection Taxonomy of Wild Tree Peony Species in China Using Pollen Characteristics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Pollen Morphology
2.3. Pollen Viability
2.4. Statistical Analysis
3. Results
3.1. Morphology of Pollen Grains
3.2. Principal Component Analysis
3.3. Pollen Viability and Germination
4. Discussion
4.1. Morphology of Pollen Grains
4.2. Principal Component Analysis
4.3. Pollen Viability and Germination
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Population | Geographic Origin | Longitude E (°) and Latitude N (°) | Elevation (m) |
---|---|---|---|---|
P. rockii | P1 | Taibai County, Shaanxi | 107.52/33.82 | 1438 |
P. rockii | P2 | Wudu District, Gansu | 105.24/33.32 | 2202 |
P. rockii | P3 | Mei Co., Shaanxi | 107.70/34.08 | 1528 |
P. rockii | P4 | Feng Co., Shaanxi | 106.46/33.94 | 1386 |
P. rockii | P5 | Liuba Co., Shaanxi | 107.15/33.75 | 1250 |
P. rockii | P6 | Ganquan Co., Shaanxi | 106.96/36.58 | 1412 |
P. ostii | P7 | Mei Co., Shaanxi | 107.71/34.07 | 1563 |
P. ostii | P8 | Shangnan Co., Shaanxi | 110.85/33.53 | 653 |
P. qiui | P9 | Shennongjia, Hubei | 110.67/31.77 | 1976 |
P. qiui | P10 | Xunyang Co., Shaanxi | 109.32/32.98 | 1558 |
P. qiui | P11 | Shangnan Co., Shaanxi | 110.63/33.40 | 1141 |
P. jishanensis | P12 | Jishan Co., Shanxi | 110.95/35.72 | 1163 |
P. decomposita | P13 | Maerkang Co., Sichuan | 102.02/32.00 | 2504 |
P. decomposita | P14 | Li Co., Sichuan | 103.45/31.58 | 2176 |
P. delavayi | P15 | Lijiang City, Yunnan | 100.17/26.80 | 3015 |
P. potaninii | P16 | Yajiang Co., Sichuan | 101.15/30.07 | 3127 |
P. lutea | P17 | Chayu Co., Tibet | 96.79/28.72 | 2980 |
P. ludlowii | P18 | Linzhi City, Tibet | 94.63/29.48 | 2958 |
Code | Shape | Amb | Exine Ornamentation |
---|---|---|---|
1 | Perprolate | Three-lobed circular | Reticulate |
2 | Prolate | Obtuse triangular | Microreticulate |
3 | - | - | Foveolate |
4 | - | - | Rugulate–reticulate |
Subsection | Species | Population | P/μm | E/μm | P/E | D/μm | W/μm | D/W | Pollen Shape | Amb | Exine Ornamentation |
---|---|---|---|---|---|---|---|---|---|---|---|
subsect. Vaginatae | P. rockii | P1 | 48.85 | 21.72 | 2.25 | 1.07 | 0.52 | 2.04 | Perprolate | Three-lobed circular | Reticulate |
P. rockii | P2 | 44.18 | 22.72 | 1.94 | 1.82 | 0.33 | 5.48 | Prolate | Three-lobed circular | Reticulate | |
P. rockii | P3 | 44.18 | 22.34 | 1.98 | 1.15 | 0.45 | 2.58 | Prolate | Three-lobed circular | Reticulate | |
P. rockii | P4 | 47.04 | 23.76 | 1.98 | 1.67 | 0.66 | 2.51 | Prolate | Three-lobed circular | Reticulate | |
P. rockii | P5 | 44.81 | 19.8 | 2.26 | 1.44 | 0.45 | 3.2 | Perprolate | Three-lobed circular | Reticulate | |
P. rockii | P6 | 45.83 | 23.05 | 1.99 | 1.14 | 0.58 | 1.97 | Prolate | Obtuse triangular | Reticulate | |
P. ostii | P7 | 44.96 | 20.02 | 2.25 | 0.59 | 0.53 | 1.11 | Perprolate | Three-lobed circular | Microreticulate | |
P. ostii | P8 | 49.25 | 21.67 | 2.27 | 0.71 | 0.43 | 1.65 | Perprolate | Three-lobed circular | Microreticulate | |
P. qiui | P9 | 48.15 | 22.28 | 1.75 | 0.89 | 0.54 | 1.02 | Perprolate | Three-lobed circular | Microreticulate | |
P. qiui | P10 | 45.11 | 19.54 | 2.31 | 0.52 | 0.5 | 1.04 | Perprolate | Three-lobed circular | Foveolate | |
P. qiui | P11 | 46.85 | 21.4 | 2.19 | 0.62 | 0.43 | 1.44 | Perprolate | Three-lobed circular | Foveolate | |
P. jishanensis | P12 | 47.51 | 22.06 | 2.15 | 1.78 | 1.22 | 1.46 | Perprolate | Three-lobed circular | Foveolate | |
P. decomposita | P13 | 47.18 | 22.58 | 2.09 | 0.71 | 0.53 | 1.35 | Perprolate | Three-lobed circular | Reticulate | |
P. decomposita | P14 | 46.11 | 22.64 | 2.04 | 0.22 | 0.61 | 0.36 | Perprolate | Three-lobed circular | Foveolate | |
subsect. Delavayanae | P. delavayi | P15 | 42.27 | 20.57 | 2.05 | 0.55 | 0.48 | 1.15 | Perprolate | Three-lobed circular | Rugulate–reticulate |
P. potaninii | P16 | 39.69 | 21.5 | 1.85 | 0.43 | 0.46 | 0.95 | Perprolate | Three-lobed circular | Foveolate | |
P. lutea | P17 | 42.58 | 21.06 | 2.02 | 0.37 | 0.43 | 0.86 | Perprolate | Three-lobed circular | Foveolate | |
P. ludlowii | P18 | 41.37 | 20.22 | 2.05 | 0.39 | 0.35 | 1.11 | Perprolate | Three-lobed circular | Foveolate |
Character | PC1 | PC2 | PC3 |
---|---|---|---|
Polar length (μm) | 0.391 | 0.723 | 0.193 |
Equatorial diameter (μm) | 0.765 | −0.093 | 0.458 |
P/E | −0.375 | 0.760 | −0.391 |
perforation diameter | 0.814 | 0.328 | −0.158 |
murus width | 0.154 | 0.573 | 0.649 |
D/W | 0.708 | −0.010 | −0.622 |
Pollen shape | 0.855 | −0.374 | 0.068 |
Amb | 0.386 | −0.178 | 0.292 |
Exine ornamentation type | −0.794 | −0.143 | 0.345 |
Eigenvalue | 3.575 | 1.736 | 1.442 |
Variance (%) | 39.772% | 19.288% | 16.026% |
Cumulative variance (%) | 39.772% | 59.009% | 75.035% |
Subsection | Population | Pollen Germination Rate (%) | Average (%) |
---|---|---|---|
subsect. Vaginatae | P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12 P13 P14 | 84.30 64.17 80.02 77.29 20.15 26.16 66.63 73.61 48.19 87.78 61.04 62.62 56.88 50.89 | 61.41 |
subsect. Delavayanae | P15 P16 P17 P18 | 25.77 36.61 46.34 32.91 | 35.41 |
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Wang, Y.; Wang, J.; Yan, Z.; Niu, L.; Zhang, X.; Zhang, Y. Optimum Discrimination on the Subsection Taxonomy of Wild Tree Peony Species in China Using Pollen Characteristics. Horticulturae 2022, 8, 736. https://doi.org/10.3390/horticulturae8080736
Wang Y, Wang J, Yan Z, Niu L, Zhang X, Zhang Y. Optimum Discrimination on the Subsection Taxonomy of Wild Tree Peony Species in China Using Pollen Characteristics. Horticulturae. 2022; 8(8):736. https://doi.org/10.3390/horticulturae8080736
Chicago/Turabian StyleWang, Yiting, Jiangnan Wang, Zhenguo Yan, Lixin Niu, Xiaoxiao Zhang, and Yanlong Zhang. 2022. "Optimum Discrimination on the Subsection Taxonomy of Wild Tree Peony Species in China Using Pollen Characteristics" Horticulturae 8, no. 8: 736. https://doi.org/10.3390/horticulturae8080736