Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Handling
2.2. Measurement of Growth Parameters
2.3. Determination of Root Activity
2.4. Determination of Chlorophyll Content
2.5. Determination and Analysis of Leaf Stomata Parameters
2.6. Determination and Analysis of Leaf Chlorophyll Fluorescence Parameters
2.7. Determination of Se and Cd Contents
2.8. Chloroplast Ultrastructure Analysis
2.9. Determination and Analysis of Electrolyte Leakage
2.10. Determination and Analysis of MDA and Proline Content
2.11. O2− and H2O2 Histochemical Staining and Content
2.12. Antioxidant Enzyme System Determination and Analysis
2.13. Statistical Analysis
3. Results
3.1. Exogenous Se Can Promote the Growth of Cabbage Seedlings under Cd Stress
3.2. Effects of Exogenous Se on Stomatal Parameters of Cabbage Seedlings under Cd Stress
3.3. Effects of Exogenous Se on Chlorophyll Fluorescence Parameters of Cabbage Seedlings under Cd Stress
3.4. Exogenous Se Can Enhance the Tolerance of Cabbage Seedlings under Cd Stress
3.5. Observation of Chloroplast Ultrastructure in Leaves of Cabbage Seedlings
3.6. Effects of Exogenous Se on Reactive Oxygen Species Accumulation and Leaf Membrane Esterification of Cabbage Seedlings under Cd Stress
3.7. Effects of Exogenous Se on Reactive Oxygen Species Accumulation and Leaf Membrane Esterification of Cabbage Seedlings under Cd Stress
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jia, K.; Zhan, Z.; Wang, B.; Wang, W.; Wei, W.; Li, D.; Huang, W.; Xu, Z. Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings. Horticulturae 2023, 9, 1016. https://doi.org/10.3390/horticulturae9091016
Jia K, Zhan Z, Wang B, Wang W, Wei W, Li D, Huang W, Xu Z. Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings. Horticulturae. 2023; 9(9):1016. https://doi.org/10.3390/horticulturae9091016
Chicago/Turabian StyleJia, Kaiyue, Zhipeng Zhan, Bingqian Wang, Wuhong Wang, Wenjing Wei, Dawei Li, Wei Huang, and Zhongmin Xu. 2023. "Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings" Horticulturae 9, no. 9: 1016. https://doi.org/10.3390/horticulturae9091016