Diverting the Use of Hand-Operated Tablet Press Machines to Bioassays: A Novel Protocol to Test ‘Waste’ Insoluble Shell Matrices
Abstract
:1. Introduction
2. Materials and Methods
2.1. Material Samples
2.2. Shell Matrix Extraction
2.3. Hand-Operated Tablet Press for AIMs
2.4. Fourier Transform Infra-Red Spectroscopy of AIMs
2.5. Antibacterial Assay
3. Results
3.1. Shell Matrix Extraction
3.2. Hand-Operated Tablet Press for Insoluble Matrix
3.3. Fourier Transform Infrared Spectroscopy
3.4. Antibacterial Assay on AIMs
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Mollusc Species | Mineralogy and Shell Microstructure * | Matrix Fraction | Mean Matrix Quantity (mg) per Gram of Shell Powder | % Matrix in Shell Powder | % AIM/Total Matrix | |
---|---|---|---|---|---|---|
Magallana gigas Pacific oyster | CALCITIC OL: prismatic IL: foliated + chalky lenses | AIM | 4.35 | 0.57% | 76.74% | |
ASM > 10 kDa | 1.14 | |||||
1 < ASM < 10 kDa | 0.18 | |||||
Cerastoderma edule Common cockle | ARAGONITIC OL: crossed-lamellar IL: complex crossed-lamellar | AIM | 0.51 | 0.08% | 65.25% | |
ASM > 10 kDa | 0.10 | |||||
1 < ASM < 10 kDa | 0.18 | |||||
Venerupis philippinarum Manila clam | ARAGONITIC OL: composite prismatic IL: crossed-lamellar becoming homogeneous | AIM | 1.18 | 0.15% | 77.96% | |
ASM > 10 kDa | 0.22 | |||||
3 < ASM < 10 kDa | 0.09 | |||||
1 < ASM < 3 kDa | 0.03 | |||||
Venus verrucosa Warty venus | ARAGONITIC OL: composite prismatic ML: crossed-lamellar IL: homogeneous | AIM | 1.11 | 0.20% | 55.23% | |
ASM > 10 kDa | 0.72 | |||||
1 < ASM < 10 kDa | 0.18 |
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Lutet-Toti, C.; Da Silva Feliciano, M.; Debrosse, N.; Thomas, J.; Plasseraud, L.; Marin, F. Diverting the Use of Hand-Operated Tablet Press Machines to Bioassays: A Novel Protocol to Test ‘Waste’ Insoluble Shell Matrices. Methods Protoc. 2024, 7, 30. https://doi.org/10.3390/mps7020030
Lutet-Toti C, Da Silva Feliciano M, Debrosse N, Thomas J, Plasseraud L, Marin F. Diverting the Use of Hand-Operated Tablet Press Machines to Bioassays: A Novel Protocol to Test ‘Waste’ Insoluble Shell Matrices. Methods and Protocols. 2024; 7(2):30. https://doi.org/10.3390/mps7020030
Chicago/Turabian StyleLutet-Toti, Camille, Marie Da Silva Feliciano, Nelly Debrosse, Jérôme Thomas, Laurent Plasseraud, and Frédéric Marin. 2024. "Diverting the Use of Hand-Operated Tablet Press Machines to Bioassays: A Novel Protocol to Test ‘Waste’ Insoluble Shell Matrices" Methods and Protocols 7, no. 2: 30. https://doi.org/10.3390/mps7020030