Methods and Protocols

Recreational dance offers significant psychological well-being potential. However, traditional instruction emphasizes technique while limiting attention to nervous system development and embodied meaning-making. Despite empirical support for polyvagal theory, motor learning science, somatic education, and phenomenology, their systematic integration into unified structures is not clearly established in recreational dance contexts. This protocol integrates nervous system regulation, motor learning, and creative expression within structured Imperial Society of Teachers of Dancing (ISTD) modern dance syllabus for recreational adults. It presents a 12-week integrated dance-mindfulness intervention addressing this gap through a three-phase structure grounded in neuroscience and embodied pedagogy. The intervention comprises eight standardized components delivered weekly. The randomized controlled trial evaluates intervention effects using the Satisfaction With Life Scale (SWLS), Depression Anxiety Stress Scales-21 (DASS-21), the Mindful Attention Awareness Scale (MAAS), the Subjective Happiness Scale (SHS), and the Leisure Involvement Scale (LIS). Qualitative assessment via semi-structured phenomenological interviews (Weeks 8 and 12) and weekly journaling captures somatic awareness, nervous system resilience, technical confidence, creative expression, relational and social belonging, and embodied meaning-making. Intervention participants are expected to show significantly greater improvements compared to controls. Results will establish evidence-based practice standards for recreational dance and demonstrate neuroscience integration’s efficacy for psychological wellbeing and embodied meaning-making.

This study aimed to establish an indirect ELISA for detecting the avian reovirus (ARV) epidemic strain xj-1.1 by using the purified recombinant protein pET-σC as the coating antigen. To optimize assay performance, key parameters were systematically evaluated, including antigen-coating concentration, serum dilution, blocking reagent and duration, serum incubation time, and the dilution and reaction time of the HRP-conjugated secondary antibody. The optimized conditions identified were a coating antigen dilution of 1:100, serum dilution of 1:1600, coating at 37 °C for 1 h followed by overnight incubation at 4 °C, and blocking with 5% skim milk for 2 h. The optimal serum incubation time was 1.5 h, with the secondary antibody diluted 1:1000 and incubated for 2 h, followed by a 20-min color development step. The cut-off value for distinguishing positive and negative samples was determined to be 0.121. Validation of the assay demonstrated favorable specificity, sensitivity, and repeatability, indicating that the developed indirect ELISA provides a reliable method for detecting ARV xj-1.1 infection.

The differentiation of human embryonic stem cells (hESCs) into primordial germ cell-like cells (PGC-LCs) provides a robust in vitro model to study human germline specification. Here, we present a simple, reproducible, and cost-effective protocol for generating DEAD-box helicase 4 (DDX4)/VASA and Deleted in Azoospermia-Like (DAZL)-positive PGC-LCs from hESCs using a combination of bone morphogenetic protein 4 (BMP4) and conditioned medium (CM) derived from Stage-Specific Embryonic Antigen-4 (SSEA4)-positive human amniotic fluid stem cells (hAFSC-4). Importantly, unlike conventional protocols that rely on embryoid body formation, our method employs adherent cultures for germ cell differentiation. This approach enhances reproducibility by avoiding the spontaneous and stochastic variability inherent to embryoid body formation. This protocol provides a reproducible and physiologically relevant platform for studying human germ cell development in vitro.