Parasitological Examination of the Digestive System of Wild Boar from a Practical Point of View—Endoparasitological Sampling under Field Conditions
Abstract
:1. Introduction
2. Design of the Foundation of Our Experiences
2.1. Location of Sample Collection
2.2. Materials and Equipment
2.2.1. Equipment for Collecting Samples on Individual Hunts
- Large foil bag (capacity of 200 L or more) or construction industry covering foil;
- Powerful (20,000 lumens), long-lasting (minimum 2–3 h) headlamp;
- Particularly sharp knife, medical scalpel;
- Sharpening steel;
- Bone saw or splitting axe;
- Several pairs of medical or thicker rubber gloves;
- Medical mask;
- Container suitable for transporting the sample and storing it until processing, or a plastic bag (thick-walled, extra strong construction industry foil bag);
- Strong, 3–4 mm thick binding twine;
- Alcoholic marker pen;
- White, writable adhesive tape;
- Pen;
- Covered writing board;
- Pre-printed sample collection data sheet, which contains the behaviour, gender, estimated age, visceral weight, condition, and health status of the wild boar that can be detected and assessed by visual inspection before the shoot.
2.2.2. Equipment for Collecting Samples on Large-Scale Hunting
- Instead of a foil bag, a collection container with a capacity of 15–20 L that can be closed with a lid;
- White insulating tape
2.2.3. Equipment Required for Sample Selection
- Forceps;
- Glass sheet (approx. 80 cm × 120 cm);
- Strainer;
- Lighting (battery or fixed power supply);
- A small or medium-sized, preferably dark-coloured bowl;
- Rubber glass cleaning paddle.
3. Detailed Procedure
3.1. The First Phase of Sample Collection
3.1.1. Collecting Samples on Individual Hunts
3.1.2. Collecting Samples during Large-Scale Hunting
3.2. The Second Phase of Sample Collection
Sampling in an Isolated Room under Controlled Conditions
4. Expected Results
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Martínez-Pérez, J.M.; Vandekerckhove, E.; Vlaminck, J.; Geldhof, P.; Martínez-Valladares, M. Serological detection of Ascaris suum at fattening pig farms is linked with performance and management indices. Vet. Parasitol. 2017, 248, 33–38. [Google Scholar] [CrossRef]
- Kliks, M.; Tantachamrun, T.; Chaiyaporn, V. Human infection by an acanthocephalan Macracanthorhynchus hirudinaceus in Thailand: New light on a previous case. Southeast Asian J. Trop. Med. Public Health 1974, 5, 303–309. [Google Scholar] [PubMed]
- Meng, X.J.; Lindsay, D.S.; Sriranganathan, N. Wild boars as sources for infectious diseases in livestock and humans. Philos. Trans. R. Soc. B Biol. Sci. 2009, 364, 2697–2707. [Google Scholar] [CrossRef]
- Majoros, G.; Juhász, A. Állatorvosi Parazitológiai Diagnosztika II. Helmintológia; Semmelweis University: Budapest, Hungary, 2020; pp. 5–13. [Google Scholar]
- Belov, Y.U.A.; Tabakaeva, T.V.; Pankratov, D.V.; Shchelkanov, E.M.; Surovyi, A.L.; Popov, I.A.; Tabakaev, A.V.; Zheleznova, L.V.; Galkina, I.V.; Shchelkanov, M.Y.U. Endoparasites of wild boars (Sus scrofa) in Primorsky Krai, Russia. Helminthologia 2022, 59, 165–169. [Google Scholar] [CrossRef] [PubMed]
- Mundim, M.J.S.; Mundim, A.V.; Santos, A.L.Q.; Cabral, D.D.; Faria, E.S.M.; Moraes, F.M. Helminths and protozoa in wild boars (Sus scrofa scrofa) feces raised in captivity. Arq. Bras. Med. Vet. E Zootec. 2004, 56, 792–795. [Google Scholar] [CrossRef]
- Dodangeh, S.; Azami, D.; Daryani, A.; Gholami, S.; Sharif, M.; Mobedi, I.; Sarvi, S.; Soleymani, E.; Rahimi, M.T.; Pirestani, M.; et al. Parasitic Helminths in Wild Boars (Sus scrofa) in Mazandaran Province, Northern Iran. Iran. J. Parasitol. 2018, 13, 416–422. [Google Scholar]
- Spieler, N.; Schnyder, M. Lungworms (Metastrongylus spp.) and intestinal parasitic stages of two separated Swiss wild boar populations north and south of the Alps: Similar parasite spectrum with regional idiosyncrasies. Int. J. Parasitol. Parasites Wildl. 2021, 14, 202–210. [Google Scholar] [CrossRef] [PubMed]
- Castagna, F.; Musella, V.; Esposito, L.; Poerio, A.; Rinaldi, L.; Bosco, A.; Cringoli, G.; Britti, D. Helminths of wild boar (Sus scrofa) in the calabrian region of southern Italy. J. Wildl. Dis. 2019, 55, 416–420. [Google Scholar] [CrossRef]
- Ilić, T.; Mihajlović, N.; Dimitrijević, S.; Bogunović, D.; Nenadović, K.; Gajić, B.; Petrović, T.; Despotović, D.; Becskei, Z. The Prevalence and Degree of Endoparasitic Infections in Wild Boars Using the Semi-quantitative Fecal Egg Count Method. Acta Parasitol. 2021, 66, 104–115. [Google Scholar] [CrossRef]
- Stojanov, I.; Pavlovic, I.; Pusic, I.; Prodanov-Radulović, J.; Ratajac, R.; Marčić, D.; Savić, B. Determination of endoparasites by faecal examination in the wild boar population in vojvodina (Serbia). Maced. Vet. Rev. 2018, 41, 39–46. [Google Scholar] [CrossRef]
- Ciocco, R.B.; Carpinetti, B.N.; Rojas, P.; Castresana, G.; Notarnicola, J. Endoparasites in a wild boar population (Sus scrofa) from Bahía Samborombón, Buenos Aires, Argentina. Rev. Mex. Biodivers. 2019, 90, 1–10. [Google Scholar] [CrossRef]
- Nagy, G.; Ács, K.; Csivincsik, Á.; Varga, G.; Sugár, L. A sertés buzogányfejű férgének előfordulása dunántúli vaddisznóállományokban egyes környezeti tényezők függvényében. Erdészettudományi Közlemények 2014, 4, 198–201. [Google Scholar]
- Nosal, P.; Kowal, J.; Wyrobisz-Papiewska, A.; Wajdzik, M. Gastrointestinal nematodes of European wild boar from distinct agricultural and forest habitats in Poland. Acta Vet. Scand. 2020, 62, 9. [Google Scholar] [CrossRef] [PubMed]
- Pavlovic, I. Helminths of Wild Boars Hunting in the Belgrade Area. Int. J. Zool. Anim. Biol. 2022, 5, 000421. [Google Scholar] [CrossRef]
- Dessì, G.; Cabras, P.; Mehmood, N.; Ahmed, F.; Porcu, F.; Veneziano, V.; Burrai, G.P.; Tamponi, C.; Scala, A.; Varcasia, A. First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights. Parasitol. Res. 2022, 121, 197–204. [Google Scholar] [CrossRef] [PubMed]
- Senlik, B.; Cirak, V.; Girisgin, O.; Akyol, C. Helminth infections of wild boars (Sus scrofa) in the Bursa province of Turkey. J. Helminthol. 2011, 85, 404–408. [Google Scholar] [CrossRef] [PubMed]
- Sarkari, B.; Mansouri, M.; Najjari, M.; Derakhshanfar, A.; Mowlavi, G. Macracanthorhynchus hirudinaceus: The most common helminthic infection of wild boars in southwestern Iran. J. Parasit. Dis. 2016, 40, 1563–1566. [Google Scholar] [CrossRef]
- Migliore, S.; Puleio, R.; Gaglio, G.; Vicari, D.; Seminara, S.; Sicilia, E.R.; Galluzzo, P.; Cumbo, V.; Loria, G.R. A Neglected Parasite: Macracanthorhynchus hirudinaceus, First Report in Feral Pigs in a Natural Park of Sicily (Southern Italy). Front. Vet. Sci. 2021, 8, 659306. [Google Scholar] [CrossRef]
- Navarro-Gonzalez, N.; Fernández-Llario, P.; Pérez-Martín, J.E.; Mentaberre, G.; López-Martín, J.M.; Lavín, S.; Serrano, E. Supplemental feeding drives endoparasite infection in wild boar in Western Spain. Vet. Parasitol. 2013, 196, 114–123. [Google Scholar] [CrossRef]
- Lahmar, S.; Torgerson, P.R.; Mhemmed, H.; Tizaoui, L.; Mhadhbi, N.; Bani, A.; Driss, H.; Ghrissi, N.; Makhzoumi, M.; Ben Houidi, A.; et al. Cystic echinococcosis and other helminth infections of wild boar in northeastern and northwestern regions of Tunisia. Parasitology 2019, 146, 1263–1274. [Google Scholar] [CrossRef]
- Petersen, H.H.; Takeuchi-Storm, N.; Enemark, H.L.; Nielsen, S.T.; Larsen, G.; Chriél, M. Surveillance of important bacterial and parasitic infections in Danish wild boars (Sus scrofa). Acta Vet. Scand. 2020, 62, 41. [Google Scholar] [CrossRef]
- Farkas, C.; Juhász, A.; Fekete, B.; Egri, B. Comparative analysis of Ascaris suum and Macracanthorhynchus hirudinaceus infections in free-ranging and captive wild boars (Sus scrofa) in Hungary. Animals 2024, 14, 932. [Google Scholar] [CrossRef]
- Varga, G. Kerti és Szabad Területi Vaddisznók Egészségi Állapotának és Parazitás Fertőzöttségének Összehasonlító Vizsgálata. Ph.D. Dissertation, West Hungarian University, Sopron, Hungary, 2006; pp. 59–68. [Google Scholar]
- Majoros, G. Állatkerti és vadállatok parazitológiai vizsgálata. Diagn. Vadállatorvoslásban 2007, 10, 59. [Google Scholar]
- Vetési, F.; Mészáros, M.J. A Háziállatok Diagnosztikai Boncolása; Mezőgazdasági Kiadó: Budapest, Hungary, 1998; pp. 31–46. [Google Scholar]
- Egri, B.; Sugár, L. Paraziták. In Vadbetegségek; Sugár, L., Ed.; Mezőgazda Kiadó: Budapest, Hungary, 2007; pp. 94–104. [Google Scholar]
- Kassai, T. Helmintológia, 2nd ed.; Medicina Könyvkiadó Rt.: Budapest, Hungary, 2003; pp. 264–265. [Google Scholar]
- Roepstorff, A.; Nansen, P. Epidemiology, Diagnosis and Control of Helminth Parasites of Swine; FAO: Rome, Italy, 1998; pp. 69–109. [Google Scholar]
- Imrich, I.; Mlyneková, E.; Mlynek, J.; Kanka, T.; Rolinec, M.; Bučko, O. Prevalence of endoparasites in the wild boars in the mountains of Pohronský Inovec. Res. Pig Breed. 2016, 10, 6–9. [Google Scholar]
- Knight, W.B.; Hiatt, R.A.; Cline, B.L.; Ritchie, A.S. A modification of the formol-ether concentration technique for increased sensitivity in detecting Schistosoma mansoni eggs. Am. J. Trop. Med. Hyg. 1976, 25, 818–823. [Google Scholar] [CrossRef]
- Mowlavi, G.R.; Massoud, J.; Mobedi, I.; Solaymani-Mohammadi, S.; Gharagozlou, M.J.; Mas-Coma, S. Very highly prevalent Macracanthorhynchus hirudinaceus infection of wild boar Sus scrofa in Khuzestan province, south-western Iran. Helminthologia 2006, 43, 86–91. [Google Scholar] [CrossRef]
- Eslami, A.; Farsad-Hamdi, S. Helminth parasites of wild boar, Sus scrofa, in Iran. J. Wildl. Dis 1992, 28, 316–318. [Google Scholar] [CrossRef] [PubMed]
Area | Reference | Year of Publication | Methods | Number of Examined Wild Boars |
---|---|---|---|---|
Turkey | [17] | 2011 | wild boars were examined by dissection | 27 wild boars |
West Spain | [20] | 2013 | wild boars were examined by dissection | 300 wild boars |
Southwest Iran | [18] | 2016 | Pathological and epidemiological studies were carried out | 25 wild boars |
Northwest Tunisia | [21] | 2019 | Dissection and faeces examination | 591 wild boars |
Denmark | [22] | 2020 | Dissection and faeces examination | 255 wild boars |
Poland | [14] | 2020 | Post-mortem examination | 57 wild boars |
Hungary | [23] | 2024 | Dissection | 173 wild boars |
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Farkas, C.; Juhász, A.; Fekete, B.; Egri, B. Parasitological Examination of the Digestive System of Wild Boar from a Practical Point of View—Endoparasitological Sampling under Field Conditions. Methods Protoc. 2024, 7, 65. https://doi.org/10.3390/mps7040065
Farkas C, Juhász A, Fekete B, Egri B. Parasitological Examination of the Digestive System of Wild Boar from a Practical Point of View—Endoparasitological Sampling under Field Conditions. Methods and Protocols. 2024; 7(4):65. https://doi.org/10.3390/mps7040065
Chicago/Turabian StyleFarkas, Csaba, Alexandra Juhász, Balázs Fekete, and Borisz Egri. 2024. "Parasitological Examination of the Digestive System of Wild Boar from a Practical Point of View—Endoparasitological Sampling under Field Conditions" Methods and Protocols 7, no. 4: 65. https://doi.org/10.3390/mps7040065