Comparison of Antigen Retrieval Methods for Immunohistochemical Analysis of Cartilage Matrix Glycoproteins Using Cartilage Intermediate Layer Protein 2 (CILP-2) as an Example
Abstract
:1. Introduction
2. Materials and Methods
Sampling
3. Antigen Retrieval Methods and IHC Staining of CILP-2
3.1. Antigen Retrieval Protocols
3.2. Description of the IHC Method for Detection of CILP-2
3.3. Formation of Study Groups and Assessment of CILP-2 Staining
3.4. Statistical Analysis
4. Results
5. Discussion
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Torga, T.; Suutre, S.; Kisand, K.; Aunapuu, M.; Arend, A. Comparison of Antigen Retrieval Methods for Immunohistochemical Analysis of Cartilage Matrix Glycoproteins Using Cartilage Intermediate Layer Protein 2 (CILP-2) as an Example. Methods Protoc. 2024, 7, 67. https://doi.org/10.3390/mps7050067
Torga T, Suutre S, Kisand K, Aunapuu M, Arend A. Comparison of Antigen Retrieval Methods for Immunohistochemical Analysis of Cartilage Matrix Glycoproteins Using Cartilage Intermediate Layer Protein 2 (CILP-2) as an Example. Methods and Protocols. 2024; 7(5):67. https://doi.org/10.3390/mps7050067
Chicago/Turabian StyleTorga, Taavi, Siim Suutre, Kalle Kisand, Marina Aunapuu, and Andres Arend. 2024. "Comparison of Antigen Retrieval Methods for Immunohistochemical Analysis of Cartilage Matrix Glycoproteins Using Cartilage Intermediate Layer Protein 2 (CILP-2) as an Example" Methods and Protocols 7, no. 5: 67. https://doi.org/10.3390/mps7050067