1. Introduction
The Chinese mitten crab (
Eriocheir sinensis) is an important aquaculture species that is widely distributed in eastern China [
1,
2,
3]. Aquaculture of this species began in the 1980s, and large-scale production began in the late 1990s [
4]. The production of
E. sinensis reached 775,900 tons in 2020 according to the China Fishery Statistical Yearbook [
5].
E. sinensis is a traditional savory food in China, and the cooked meat has a uniquely pleasant aroma and delicious taste. Therefore, it is very popular among Chinese consumers [
6].
Largemouth bass (
Micropterus salmoides) is native to North America and was introduced to China in the 1980s. Due to its delicious meat, high nutritional value, rapid growth, short breeding period, and strong adaptability, it quickly became one of the most important freshwater breeding varieties in China [
7,
8,
9,
10,
11].
The growth, physiological statuses, and nutrient compositions of Chinese mitten crabs and largemouth bass are affected by many factors, including stocking density, feeding, and aquaculture method [
12,
13,
14,
15], and different farming techniques can improve the culture water environment. Because aquatic animals in an ideal polyculture pond occupy different ecological niches and have complementary feeding habits, farmers are often advised to develop polyculture systems to increase their productivity [
16,
17]. It is generally accepted that polyculture provides higher yields, and it is considered more ecologically healthy than monoculture [
18,
19]. Based on the common feeding habits and different habitats of Chinese mitten crabs and largemouth bass, the bass–crab polyculture model was developed. This polyculture model can significantly increase the output of aquaculture products, provide ecological benefits, efficiently and fully utilize space, form a mutually beneficial ecosystem, reduce morbidity, and improve the cultural environment [
20].
The goal of this study was to compare the growth, physiological status, nutritional composition, and flavor quality of E. sinensis and M. salmoides between monoculture and polyculture modes. We measured the growth parameters and antioxidant activities at four time points. We also examined differences in nutritional value and flavor between the two culture modes by determining the proximate, fatty acid, and amino acid compositions of edible tissues using the samples collected at the last time point.
2. Materials and Methods
2.1. Ethics Statement
The experimental protocol was performed following the guidelines approved by the Institutional Animal Care and Use Committee of the Ministry of Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences.
2.2. Experimental Design
The experiment was conducted at Yangzhong Experimental Base (Zhenjiang, China), Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences in January and November 2020. The Chinese mitten crabs used in the experiment came from Suzhou Youhua Ecological Technology Co., Ltd. (Suzhou, China). Largemouth bass were purchased from Anhui Zhanglin Fishery Co., Ltd. (Tongling, China)
Nine semi-enclosed ponds with convenient irrigation and drainage and consistent specifications were randomly selected. Each pond was (length × width × height = 75 m × 23 m × 1.5 m); microporous aeration pipes were installed at the bottoms of the ponds. We set up three groups in this study, each with three replicates: E. sinensis monoculture (EM), M. salmoides monoculture (MM), and polyculture of the two species (EP). The EM and MM were used as the respective control groups for the two species. Healthy and similar-sized crabs (12.53 ± 3.25 g) were stocked at 0.7 individuals per m2 in six of the ponds. Three of these ponds were randomly selected and stocked with largemouth bass (184.62 ± 2.74 g) at a density of 2.2 individuals per m2. The other three ponds were stocked only with largemouth bass at the same density.
Crabs were fed at 17:00 daily with a commercial diet (
Table 1; Nanjing Aohua Biotechnology Co., Ltd., Nanjing, China). Largemouth bass were fed two times a day at 7:00 and 17:00, respectively, with a commercial diet (
Table 1; Jiangsu Hipore Feed Co., Ltd., Xinghua, China). To detect water quality, we collected water samples every three days. During the rearing period, the dissolved oxygen of the pond was 8.24 ± 0.51 mg/L, the transparency was about 40 ± 10 cm, pH was maintained at 8.0 ± 0.5, ammonia nitrogen was 0.16 ± 0.03 mg/L, and nitrite nitrogen was 0.01 ± 0.01 mg/L.
2.3. Sampling Protocol
Samples of crabs were collected on 9 May (T1), 6 June (T2), 10 July (T3), and 10 October 2020 (T4). A total of 30 crabs (5 female crabs and 5 male crabs from each replicate pond) were randomly sampled on each date from the EM and EP groups. After being anesthetized on ice, the following measurements were taken: wet weight (using an electronic scale, accurate to 0.01 g) and carapace length and width (using a digital caliper, accurate to 0.01 mm). Next, the hepatopancreas, gonad, and muscle from each crab were dissected carefully and weighed separately. These tissues were frozen in liquid nitrogen immediately and stored at –80 °C for later analysis. From the EM and EP groups, we collected an additional 18 crabs (3 female crabs and 3 male crabs from each of the three replicate ponds) from each time point for quality analysis of edible tissues.
Samples of the largemouth bass were collected on 9 May (T1), 6 June (T2), and 10 October 2020 (T4). Fish were fasted for 24 h before sampling. At each time point, a total of 45 fish (15 fish from each replicate pond) were caught randomly from the MM and EP groups and anesthetized with MS-222 (200 mg/L). Prior to dissection, each fish was weighed and measured to obtain body height, standard length, and body width. Blood samples were collected from the caudal vein using a hypodermic syringe. The blood samples were transferred to a centrifuge tube without anticoagulant and centrifuged to obtain serum samples. Hepatopancreas and muscle samples were also collected from those fish, frozen in liquid nitrogen, and stored at –80 °C for later analysis. From the MM and EP groups, we collected an additional 18 fish (6 from each of 3 replicate ponds) from each time point for quality analysis of edible tissues.
The gonadal somatic index (GSI) and hepatosomatic index (HSI) of the crabs were calculated as follows: GSI (%) = 100 × gonadal weight/body wet weight, and HSI (%) = 100 × hepatosomatic weight/body wet weight. The meat yield (MY) of the crabs was calculated as MY (%) = 100 × muscle wet weight/body wet weight, and total edible yield (TEY, %) was calculated as the sum of GSI, HSI, and MY. The condition factor (CF[g/cm
3]) was calculated as the body wet weight/carapace length
3 [
21].
The relevant parameters of the largemouth bass were calculated as follows: HSI (%) = 100 × hepatopancreas weight/final body weight; viscerosomatic index (VSI, %) = 100 × viscera weight/body weight; weight gain (WG, %) = 100 × (W
t − W
0)/W
0; and (CF[g/cm
3]) = body weight/body length
3. W
t is the final body weight (g), and W
0 is the initial body weight (g) [
22].
2.4. Enzyme Activity Assays
To evaluate the physiological status of the crabs and fish, we measured enzyme activity in the hepatopancreas. We used ice-cold physiological saline at 0.9 mL saline per 0.1 g tissue to treat the tissue samples and then centrifuged them at 2500 rpm for 10 min. The supernatant was collected to analyze the content of malondialdehyde (MDA; A003-1), the activities of glutathione peroxidase (GSH-Px; A005), superoxide dismutase (SOD; A001-3) and catalase (CAT; A007-1), acid phosphatase (ACP; A060-2), and alkaline phosphatase (AKP; A059-2) using kits and following the manufacturer’s instructions [
23]. We used Coomassie brilliant blue staining assay kits (A045- 2-2) to quantify total protein. All kits were purchased from Nanjing Jiancheng Biology Engineering Institute (Nanjing, Jiangsu, China).
2.5. Biochemical Analysis
To detect differences in the edible tissues between culture modes, we compared the proximate composition and fatty acid composition of crab and largemouth bass edible tissues and the amino acid composition of the muscle of largemouth bass (EM vs. EP and MM vs. EP). Moisture, ash, total lipid, and crude protein contents of edible tissues were measured using standard methods. Briefly, these tissues were dried to constant weights at 105 °C to determine their moisture content [
24], while the crude protein content was determined using the Kjeldahl method (using a 6.25 N to protein conversion factor). The ash content was determined using a muffle furnace at 550 °C to a constant weight [
25], while the total lipid content was measured by Soxhlet extraction with petroleum ether [
26].
Fatty acid composition, amino acid composition, and flavor-enhancing nucleotides of edible tissues were evaluated at the National Key Laboratory of Food Science and Technology, Jiangnan University (Wuxi, Jiangsu province, China) [
27]. For fatty acid analysis, fatty acid methyl esters were prepared by transesterification with 2 M potassium hydroxide in methanol for 20 min. After centrifugation for 5 min at 805× g, the upper organic layer was diluted with hexane and used for gas chromatography analysis. Fatty acid methyl esters were analyzed using an Agilent 7820A gas chromatograph (Agilent, Santa Clara, CA, USA) equipped with a hydrogen flame ionization detector [
28]. The fatty acid composition was expressed as a percentage of each fatty acid to the total fatty acids (%). After hydrolysis by 6 M hydrochloric acid, the amino acid composition of muscle samples was determined following the general rules for amino acid analysis (JY/T019-1996) using an Agilent 1100 HPLC system [
29].
2.6. Statistical Analysis
Normal distribution and homogeneity of variance of data were tested using Shapiro–Wilk and Levene tests (α = 0.05), respectively. When necessary, logarithmic transformation was performed before data analysis. For enzyme activity and fatty acid and nutrient contents, we used independent sample t-tests to test for potential differences between the monoculture and polyculture modes. p < 0.05 was considered statistically significant. All statistical analyses were performed using IBM SPSS Statistics 26.0 (IBM Inc., Armonk, NY, USA).
4. Discussion
In recent years, increased pond stocking density, relatively simple culture mode structure, and excess feed have resulted in the deterioration of the aquaculture water environment and the frequent occurrence of diseases, which have negatively affected the development of the aquaculture industry. Therefore, culture mode in aquaculture has become an urgent issue in the aquaculture industry. The purpose of this study was to develop a mutually beneficial ecosystem for Chinese mitten crabs and largemouth bass culture using the green and healthy polyculture mode and to evaluate the potential benefits of this culture mode on crab and fish production.
We did not detect a significant difference in the growth parameters of female crabs between the monoculture and polyculture modes, but the growth parameters in male crabs were significantly higher in the EP group compared with the EM group. The final body weight and weight gain rate of largemouth bass in the EP group were significantly higher than those in the EM group. Costa et al. previously reported that the polyculture of shrimp and mullet reared together in earthen ponds favors the production of mullets [
30]. Generally, many benefits have been achieved in aquatic polyculture systems when using fish, bivalves, and seaweeds, including improved growth [
31]. The results of our study show that the polyculture mode can improve the specifications of commercial Chinese mitten crabs, especially male crabs, and those of largemouth bass size.
Similar to higher vertebrates, fish are susceptible to the effects of reactive oxygen species and have innate and effective antioxidant defenses [
32]. Crustaceans have no adaptive immune system, so their antioxidant defense systems play an important role in coping with environmental stress. The antioxidant defense system of crustaceans consists of enzymatic and non-enzymatic antioxidant systems that protect organisms against oxidative stress and damage. SOD, CAT, and GSH-Px are important antioxidants [
33,
34]. SOD is a key antioxidant that can protect living cells from the oxidative damage of superoxide radicals by catalyzing the conversion of superoxide anion radicals (O
2−) into H
2O
2 and O
2. GSH-Px is one of the main protectors against lipid peroxidation damage, and it promotes the decomposition of H
2O
2 and reduces toxic peroxides to non-toxic hydroxyl compounds. CAT can also convert H
2O
2 to water in order to thoroughly clear oxyradicals [
35,
36]. MDA, the final breakdown product of lipid peroxides, has strong cytotoxicity, thus its content is often used to assess the degree of oxidative damage to cells or tissues and the body’s antioxidant capacity [
37].
The antioxidant levels of crustaceans change depending on environmental conditions. Wang et al. showed that under high salinity stress the antioxidant level of Chinese mitten crabs increased significantly [
38]. In our study, we detected few significant differences in the antioxidant system of crabs between the two culture modes, indicating that polyculture would not cause oxidative stress damage to the crabs. For largemouth bass, the SOD activity in the EP group was always highest and the CAT activity in the EP group was significantly higher than that in the MM group at T2, which indicated that the antioxidant capacity of largemouth bass was higher under the polyculture mode. These results suggest that the polyculture of crabs and largemouth bass can activate the antioxidant system, increase the activity of GSH-Px, and act synergistically with SOD and CAT to reduce and block the damage caused by lipid peroxidation.
Because crustaceans lack an efficient adaptive immune system, ACP and AKP act as molecular effectors to protect them from pathogens and oxidative stress [
39,
40]. In the immune defense system, ACP and AKP directly regulate the transfer of phosphate groups and the metabolism of phosphate, which can accelerate the uptake and transport of substances in the body, form a hydrolase system, and destroy and remove foreign substances that invade the body, which is of great significance for maintaining the survival and growth of crabs and largemouth bass [
41,
42]. ACP is a marker enzyme of macrophage lysosomes and one of the most representative hydrolases in macrophages. As an immune indicator, AKP is a glycoprotein with a metallophosphatase structure. Its activity is a useful bioindicator of the physiological health of cellular membranes, cell growth, apoptosis, cell migration, cellular metabolic status, hepatocyte function, and detoxification activity in hepatocytes [
43,
44].
In this study, the difference in ACP activity between the EM and EP culture modes in female crabs and between the MM and EP modes in largemouth bass at T1 may be part of an adaptation process in the pond. The AKP activity of female crabs in the EM group was higher than that in the EP group, but the difference was not significant. This may indicate that mixed culture with largemouth bass has little effect on the innate immunity of crabs. The AKP activities in male crabs in both groups at T4 were significantly higher than those of the other time points. The AKP activity of largemouth bass in the EP group was significantly higher than that in the MM group at both T1 and T2. Chen et al. previously showed when affected by salinity, the increase in ACP and AKP activities helps boost the non-specific immunity of aquatic organisms [
45]. Our results show that the polyculture mode can improve the phosphatase activity of crabs and largemouth bass, which can help maintain their health.
Compared with Chinese mitten crabs collected at the beginning of the study, the proximate composition of the edible tissues did not change significantly over time. However, at the end of the experiment, the ash content in the dorsal muscle of largemouth bass in the EP group was significantly higher than that in the MM group. Ash is a measure of the mineral content of a food item [
46], thus the mineral content in the dorsal muscle of largemouth bass in the EP group was higher than that in the MM group. This result may be due to the fish ingesting part of the crab feed and chilled fish in the polyculture mode.
Fatty acids are not only nutritionally important, but they also have a major impact on meat flavor because they influence the taste [
47]. Moreover, essential fatty acids are an important basis for evaluating the nutritional quality of aquatic products [
48]. SFAs mainly provide energy to animals [
49]. In the present study, the content of ∑SFA in the edible tissues of female crabs in the EP group was higher than that in the EM group. This may be explained by the crabs’ need for more reserved energy to cope with intense competition. The PUFA/SFA ratio is often used to evaluate the nutritional quality of meat, with a high ratio indicating good quality. The PUFA/SFA ratio of male crabs was higher than that of female crabs, indicating a higher nutritional quality of the edible tissues of male crabs. The content of PUFAs C18:2 and C20:2 in largemouth bass in the EP group were significantly higher than those in the MM group, but the content of C14:0 in the EP group was lower than that in the MM group. Fatty acid consumption and energy transfer occur in fish during exercise [
50], and this may explain the differences in fatty acid content in the dorsal muscles of largemouth bass reared in the different culture modes.
Among the indicators of the quality of aquatic products, the ratio of EEAs to total hydrolyzed amino acids (E/T) is extremely important. According to the essential amino acid model of protein nutritional value proposed by the World Health Organization and the United Nations Food and Agriculture Organization in 1973, the EAA/(EAA + NEAA) value in an ideal protein should be about 40% [
51]. The ratio in the muscle of crabs in the EM and EP groups in this study was close to this ideal value, which indicates that the amino acid nutritional value of crab muscle is rich. The hydrolyzed amino acids in the muscles of female and male crabs were all high in Glu, Leu, and Asp. Glu and Asp have a sour taste, and they are important prerequisite substances for the formation of umami substances [
52]. Leu is slightly bitter and mainly plays a role in improving food flavor [
53]. In largemouth bass, the content of hydrolyzed amino acids in the EP group was not significantly different from that in the MM group. From the perspective of hydrolyzed amino acids, the polyculture mode did not affect the quality of crab or largemouth bass amino acids.
Amino acids are the basic unit of protein composition and serve as an important nutritional index in the evaluation of the meat quality of aquatic products [
54,
55]. FAAs have different tastes, and the tastes are diverse but can be divided into three types: umami, sweet, and bitter [
56]. Glu and Asp have an important impact on the umami taste of fish meat [
57]. Gly and Ala provide a sweet taste and also reduce bitterness, remove unpleasant tastes, and improve the overall taste [
58]. For example, Ala was found to improve the flavor and taste of hake (
Eleginus gracilis) [
59]. In the present study, Ala, Arg, and Pro were the most abundant in FAAs in Chinese mitten crabs. The overall levels of Ala and FAAs in crabs in the EP group did not differ significantly compared with the EM group, indicating that the polyculture mode may not affect the flavor of FAAs in their edible tissues. The Arg content of female crabs was higher in the EP group than in the EM group, indicating that polyculture was favorable for the accumulation of Arg. The contents of Glu, Asp, and Met in largemouth bass in the EP group were lower than those in the MM group, and there was no significant difference in total FAAs between the EP and MM groups. From the perspective of FAAs, the flavor of largemouth bass may be lost to a certain extent in the crab–bass polyculture mode.
Flavor nucleotides have a synergistic effect on flavor taste when they coexist with FAAs [
60]. For example, AMP can inhibit bitterness, and IMP and GMP can improve the umami taste [
61,
62]. In this study, both female and male crabs had high levels of AMP in both the EM and EP groups, and the IMP content of female crabs in the EP group was slightly higher than that in the EM group. The high content of AMP, IMP, Arg, Gly, Ala, and Glu have strong effects on crab meat flavor, and IMP nucleotides can act synergistically with Glu to enhance its perception [
63]. Thus, we suggest that the polyculture mode may help increase the umami and sweet taste of the edible parts of female crabs. Interestingly, the largemouth bass dorsal muscle had higher GMP in the EP group than in the MM group. Given that GMP is known to produce umami taste, the umami of largemouth bass was improved by polyculture.