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Evaluation of Fish Biodiversity in Estuaries Through Environmental DNA Metabarcoding: A Comprehensive Review
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Comparing Chemoautotrophic and Heterotrophic Biofloc Systems for Integrated Cultivation of Shrimp and Tilapia
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Artificial Induction of Spawning in Threeline Grunt, Parapristipoma trilineatum Under Controlled Environmental Conditions
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Distribution, Occupancy, and Habitat of the Endangered Carolina Madtom: Implications for Recovery of an Endemic Stream Fish
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Ultimate Fighting Crab: Agonistic Behaviour, Dominance, and Recognition in the Edible Crab, Cancer pagurus (L.)
Journal Description
Fishes
Fishes
is an international, peer-reviewed, scientific, open access journal published monthly online by MDPI. It covers fishes and aquatic animals research. The Iberian Society of Ichthyology (SIBIC) and the Brazilian Society of Aquaculture and Aquatic Biology (Aquabio) are affiliated with Fishes and their members receive a discount on the article processing charges.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), PubAg, FSTA, and other databases.
- Journal Rank: JCR - Q2 (Marine and Freshwater Biology)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 17.4 days after submission; acceptance to publication is undertaken in 2.6 days (median values for papers published in this journal in the second half of 2024).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
- Testimonials: See what our editors and authors say about Fishes.
- Companion Journal: Aquaculture Journal
Impact Factor:
2.1 (2023);
5-Year Impact Factor:
2.4 (2023)
Latest Articles
Effects of Glutamate on Growth Performance, Gut Digestion and Antioxidant Capacity in Juvenile Little Yellow Croaker
Fishes 2025, 10(4), 188; https://doi.org/10.3390/fishes10040188 (registering DOI) - 21 Apr 2025
Abstract
This study is to explore the alleviating effects of glutamate on intestinal damage in cultured little yellow croaker. A total of 900 juvenile fish at a weight of 30.68 ± 0.12 g were randomly separated into six groups with three replicates each, and
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This study is to explore the alleviating effects of glutamate on intestinal damage in cultured little yellow croaker. A total of 900 juvenile fish at a weight of 30.68 ± 0.12 g were randomly separated into six groups with three replicates each, and were fed a basic diet (protein at 44.42% and lipid at 12.48%) with additional glutamate at 0.00%, 0.40%, 0.80%, 1.2%, 1.60%, and 2.00%. Each replica group consisted of 50 fish in a breeding barrel (radius 1.0 m, height 1.5 m), and the experiment lasted for 54 days. The results showed that supplementation with 0.4–1.2% glutamate significantly improved the survival rate, which increased from 75.56% to 91.11%, reduced the feed conversion rate from 1.75 to 1.57, and increased the protein efficiency ratio from 1.55 to 1.85 (p < 0.05). In the intestines, the addition of 0.40–1.2% glutamate increased muscle thickness and villus height (p < 0.05), as well as the activities of pepsin, trypsin, α-amylase, and lipase (p < 0.05). Enzyme activity analysis indicated that the addition of 0.4–1.2% glutamate in the feed significantly enhanced the activities of serum Total Superoxide Dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GPx) antioxidant enzymes (p < 0.05). Transcriptome analysis of the 1.2% and 0% groups revealed that differentially expressed genes were enriched in glutathione synthesis, nutrient absorption and metabolism, and viral protein interaction with cytokine and cytokine receptor pathways. qPCR experiments verified that the addition of 1.2% glutamate significantly up-regulated the expression of antioxidant-related genes, including glutathione synthetase and Nrf2. In conclusion, the addition of glutamate can enhance growth performance, increase intestinal digestive capacity, activate intestinal glutathione synthesis to alleviate intestinal damage, and maintain intestinal health.
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(This article belongs to the Special Issue Application of Protein and Amino Acid in Aquaculture Feed)
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Typology of Fishing Grounds for Communal Fisheries Business in Korea: A Statistical Approach
by
Ji Eun An and Chang Mo Ma
Fishes 2025, 10(4), 187; https://doi.org/10.3390/fishes10040187 - 19 Apr 2025
Abstract
This study statistically classifies Korea’s communal fishing grounds (Maeul-Eojang) to inform tailored fisheries policy. We applied principal component analysis (PCA) to reduce 17 socio-economic and demographic indicators into five core factors, followed by K-means clustering to derive distinct types. The methodology was validated
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This study statistically classifies Korea’s communal fishing grounds (Maeul-Eojang) to inform tailored fisheries policy. We applied principal component analysis (PCA) to reduce 17 socio-economic and demographic indicators into five core factors, followed by K-means clustering to derive distinct types. The methodology was validated using Kaiser–Meyer–Olkin and Bartlett’s tests. Five communal fishery types were identified, ranging from well-managed, high-income communities to those in severe decline. The results show that about half of fishing communities fall into a “post-fishery” type with diminishing membership and income, while a quarter maintain robust fisheries through diversification. The typology is compared with previous fishing village classifications, and we discuss policy recommendations for each type—including co-management, tourism support, and targeted aid for declining communities. This research provides an empirical foundation for improving communal fisheries governance and sustaining coastal livelihoods.
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(This article belongs to the Section Fishery Economics, Policy, and Management)
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Effects of Climate Change on Korea’s Fisheries Production: An ARDL Approach
by
Hoonseok Cho, Pilgyu Jung and Mingyeong Jeong
Fishes 2025, 10(4), 186; https://doi.org/10.3390/fishes10040186 (registering DOI) - 18 Apr 2025
Abstract
This study investigates the impact of rising sea surface temperature (SST), increasing carbon dioxide (CO2) emissions, and precipitation variability (PREC) on Korea’s coastal and offshore fisheries production (COFP) from 1993 to 2023 using an autoregressive distributed lag (ARDL) model. The results
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This study investigates the impact of rising sea surface temperature (SST), increasing carbon dioxide (CO2) emissions, and precipitation variability (PREC) on Korea’s coastal and offshore fisheries production (COFP) from 1993 to 2023 using an autoregressive distributed lag (ARDL) model. The results confirm a long-run cointegration relationship, where a 1% increase in SST, CO2, and PREC is associated with respective declines of 3.52%, 0.82%, and 0.34% in COFP, respectively, suggesting persistent negative effects of ocean warming, acidification, and hydrological variability on fisheries production. Robustness checks using Fully Modified Ordinary Least Squares (FMOLS) and Canonical Cointegrating Regression (CCR) validate the stability of the ARDL results. The short-run analysis reveals that past production levels significantly influence current COFP, while SST fluctuations exhibit delayed but economically meaningful effects. The error correction term (−0.75, p < 0.01) confirms a rapid adjustment toward equilibrium following short-term deviations. These findings underscore the necessity of climate-resilient fisheries management. Policy recommendations include adaptive harvest regulations, climate-integrated stock assessments, and enhanced international cooperation for transboundary fish stocks. Additionally, expanding Marine Protected Areas, promoting climate-resilient aquaculture, and strengthening stock enhancement programs through selective breeding and seed release of climate-adapted species are essential for sustaining fisheries under climate change.
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(This article belongs to the Special Issue Effects of Climate Change on Marine Fisheries)
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A Comparison of White Muscle Quality in Grass Carp Ctenopharyngodon idellus Fed with Commercial Feeds and Barley Malt
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Chongjiang Hu, Zheng Huang, Hongkang Liu, Dingrui Mo, Peng Fu, Haiyan Guo, Wei Jiang, Yong Xie and Yongjun Chen
Fishes 2025, 10(4), 185; https://doi.org/10.3390/fishes10040185 - 18 Apr 2025
Abstract
This study was the first report regarding the application of barley malt (BM) for diets of aquaculture species. Triplicate groups of grass carp Ctenopharyngodon idellus with an initial size of about 1.2 kg were selected and fed with either BM or commercial feed
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This study was the first report regarding the application of barley malt (BM) for diets of aquaculture species. Triplicate groups of grass carp Ctenopharyngodon idellus with an initial size of about 1.2 kg were selected and fed with either BM or commercial feed (CF) to apparent satiation for 8 weeks in outdoor ponds connected with a flow-through aquaculture system. The results showed that the final body weight (1651 g) was lower in the BM fish than in the CF fish (1791 g). The edible part was lower in the BM fish than in the CF fish as indicated by the viscerosomatic index. Except for ash levels, which were lower in the fillet of the BM fish than for that of the CF fish, moisture, protein, and lipid levels were not impacted by the application of BM. Water-holding capacity indicators (drop loss, frozen exudation rate, and cooking loss) of grass carp muscle were not relevant to dietary modifications. Hematoxylin-eosin (HE) staining showed that the diameter of the myofibers was decreased while density was increased in response to the application of BM, which contributed to the improvement in textural properties (hardness, gumminess, and chewiness) in the muscle of the BM fish as compared to the CF fish. Glutamic acid level was highest, followed by aspartic acid, lysine, leucine, alanine, and arginine in grass carp muscle. Except three amino acids (proline, phenylalanine, and histidine), the amounts of the other 15 amino acids, essential amino acids, semi-essential amino acids, nonessential amino acids, and delicious amino acids were not impacted by different treatments, suggesting that the application of BM had a minor effect on the amino acid composition of grass carp muscle. Oleic acid (C18:1n-9), linoleic acid (C18:2n-6), and palmitic acid (C16:0) were the most abundant fatty acids in grass carp muscle. The amounts of poly-unsaturated fatty acid (PUFA) in the muscle decreased in response to the application of BM as the diet of grass carp, and n-6 PUFAs (C18:2n-6 and C20:2n-6) rather than n-3 PUFAs accounted for this change, which is beneficial for human health. In conclusion, the application of BM had minor impacts on the proximate composition and amino acid composition but improved textural properties and decreased n-6 PUFAs in the fillet of grass carp.
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(This article belongs to the Special Issue Growth, Metabolism, and Flesh Quality in Aquaculture Nutrition)
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The Effect of the Marine Environment on the Distribution of Sthenoteuthis oualaniensis in the East Equatorial Indian Ocean
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Shigang Liu, Liyan Zhang, Peng Lian, Jianhua Kang, Puqing Song, Xing Miao, Longshan Lin, Rui Wang and Yuan Li
Fishes 2025, 10(4), 184; https://doi.org/10.3390/fishes10040184 - 17 Apr 2025
Abstract
Sthenoteuthis oualaniensis is one of the most commercially important marine cephalopod species distributed throughout tropical and subtropical waters of the Indo-Pacific Seas. The Indian Ocean is a main fishing ground for S. oualaniensis with a high population density. To explore the distribution of
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Sthenoteuthis oualaniensis is one of the most commercially important marine cephalopod species distributed throughout tropical and subtropical waters of the Indo-Pacific Seas. The Indian Ocean is a main fishing ground for S. oualaniensis with a high population density. To explore the distribution of S. oualaniensis in the east equatorial Indian Ocean, four surveys were carried out using light-lift-net fishing vessels. Meanwhile, marine environmental data were also collected, including the sea surface temperature, sea temperature at 100 m depth, mixed layer depth, sea surface chlorophyll-a concentration, sea surface height, and eddy kinetic energy. Generalized Additive Models were used to analyze the relationship between the catch per unit effort (CPUE) for S. oualaniensis and environmental factors. The results showed that the average CPUE of S. oualaniensis was 14.55 kg/h in the four surveys, which was considerably lower than in the South China Sea and Northwest Indian Ocean. In terms of seasonal distribution, the high-CPUE stations were closer to the continental shelf in spring, while they shifted towards the deeper and offshore water in autumn, demonstrating a seasonal migration trend. Pearson correlation analysis showed that CPUE reflected a significant negative correlation with both sea temperature at 100 m depth and eddy kinetic energy (p < 0.001). The Generalized Additive Models revealed that sea surface height was the most significant factor affecting CPUE with a variance explanation of 30.1%. Furthermore, the optimal CPUE prediction model was established by stepwise regression, which contains two factors, sea surface height and eddy kinetic energy, with a variance explanation of 34.9%. This study provides insights into the environmental factors influencing the distribution of S. oualaniensis, which is essential for the sustainable utilization and management of this species.
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(This article belongs to the Special Issue Assessment and Management of Fishery Resources)
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Optimizing Cryopreservation of Pseudoplatystoma magdaleniatum Semen: Evaluation of Two Permeable and Two Non-Permeable Cryoprotectants
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César Montes-Petro, Betty Rodríguez-Peroza, Diana Madariaga-Mendoza, Carlos Tapia-Pacheco, José Espinosa-Araujo and Víctor Atencio-García
Fishes 2025, 10(4), 183; https://doi.org/10.3390/fishes10040183 - 17 Apr 2025
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The study aimed to evaluate the effectiveness of the cryopreservation protocols for Pseudoplatystoma magdaleniatum semen using dimethyl sulfoxide (DMSO) or methanol (MET) as permeable cryoprotectants at two concentrations (5% and 10%) combined with 12% egg yolk (Y12%) or 5% skimmed milk powder (SMP5%)
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The study aimed to evaluate the effectiveness of the cryopreservation protocols for Pseudoplatystoma magdaleniatum semen using dimethyl sulfoxide (DMSO) or methanol (MET) as permeable cryoprotectants at two concentrations (5% and 10%) combined with 12% egg yolk (Y12%) or 5% skimmed milk powder (SMP5%) and glucose (6%), resulting in eight treatments. A semen pool (n = 8) was diluted in a 1:4 ratio, packed in 2.5 mL straws, and frozen in nitrogen vapors. It was thawed at 35 °C for 90 s. Sperm kinetics and motility duration of fresh, prefrozen, and thawed semen were analyzed using a CASA system. The osmolarity of seminal plasma and cryosolutions was estimated. Fertilization (F) and embryo viability (E) rates of thawed semen were evaluated. The osmolarity of seminal plasma was 251.1 ± 3.3 mOsmol/kg and, in the cryosolutions, ranged between 1248.3 ± 19.9 mOsmol/kg (DMSO5% + Y12%) and 3488.2 ± 1.5 mOsmol/kg (MET10% + Y12%). After thawing, total motility ranged from 38.2% to 60.5%, representing a significant reduction compared to fresh semen (95.4 ± 2.1%) (p < 0.05). The best fertilization and embryo viability rates of thawed semen were obtained with DMSO5% + SMP 5% (F = 20.7%, E = 11.7%) and MET10% + SMP5% (F = 20.1%, E = 11.5%) (p < 0.05). A cryopreservation protocol for P. magdaleniatum semen with 5%DMSO or 10%MET combined with SMP5% is possible, but further study is necessary to optimize its fertilizing capacity.
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Feeding Habits of Mene maculata (Teleostei: Menidae) in the Southwestern Waters of Taiwan, Western Pacific Ocean
by
Yi-Chen Wang, Ming-An Lee and Jia-Sin He
Fishes 2025, 10(4), 182; https://doi.org/10.3390/fishes10040182 - 16 Apr 2025
Abstract
This study investigated the feeding habits of moonfish (Mene maculata) in the waters of southwestern Taiwan in the Western Pacific Ocean using commercial trawling nets and Taiwanese purse seines. For this, we analyzed the body size and stomach content of moonfish
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This study investigated the feeding habits of moonfish (Mene maculata) in the waters of southwestern Taiwan in the Western Pacific Ocean using commercial trawling nets and Taiwanese purse seines. For this, we analyzed the body size and stomach content of moonfish specimens collected from the study area between January and December 2023. The length–weight relationship revealed hypoallometric growth patterns in both male and female specimens, with allometric coefficients of 2.6574 and 2.7219, respectively. Stomach content analysis indicated that the specimens primarily fed on Calanoida (zooplankton; %PSIRI = 36.79) and Benthosema pterotum (fish; %PSIRI = 19.23). Dietary composition varied with body size and sampling time. Larger individuals consumed higher proportions of fish. The percentage of empty stomachs was high, likely attributable to the fishing gear used and sampling time. Based on the results of this study, it is speculated that moonfish feed mainly before early morning or at other times during the day.
Full article
(This article belongs to the Special Issue Fish Trophic Ecology: Revealing the Responses to Global Change from Individuals to Ecosystems)
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The Impact of Feeding Rates on the Growth, Stress Response, Antioxidant Capacity, and Immune Defense of Koi (Cyprinus carpio var. koi)
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Saixing Duan, Shengyu Gao, Xiaolong Xu, Qi Wei, Ze Tao, Gaoxiao Xu, Quanzhou Liu, Bing Wei and Chaofan He
Fishes 2025, 10(4), 181; https://doi.org/10.3390/fishes10040181 - 16 Apr 2025
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The feeding rate is critical for fish growth and the feed conversion ratio (FCR). Prior research has primarily evaluated the optimal feeding rate by examining growth performance and the FCR. Given the high cost of koi as an ornamental fish, it is essential
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The feeding rate is critical for fish growth and the feed conversion ratio (FCR). Prior research has primarily evaluated the optimal feeding rate by examining growth performance and the FCR. Given the high cost of koi as an ornamental fish, it is essential to consider the effects of feeding rates on its welfare and health. This study aimed to examine the impact of the feeding rate on the growth, stress response, antioxidant capacity, and immune defense of koi. A total of 240 fish, with an initial body weight of 10.02 ± 0.18 g, were randomly assigned to six groups and fed expanded diets at 1%, 2%, 3%, 4%, 5%, and 6% of body weight per day (BW/day) for a duration of eight weeks. The study results indicated that the daily weight gain of fish at 3–4% BW/day was significantly greater than that of the other groups. Fish in both high and low feeding rate groups exhibited reduced antioxidant capacity and heightened inflammatory response, as indicated by a notable decrease in catalase (CAT) and superoxide dismutase (SOD) activity, along with diminished expression of cat and sod, as well as a significant increase in the expression of tnf-α, il1, and il10. The elevated feeding rate did not enhance immune defense mechanisms. Furthermore, a low feeding rate resulted in heightened stress and reduced immunity in koi, as indicated by a significant reduction in plasma cortisol (COR), complement C3, complement C4, total protein (TP), albumin (ALB), and globulin (GLO) levels, along with decreased activities of lysozyme (LZM) and myeloperoxidase (MPO). The optimal feeding rate for enhancing non-specific immunity in koi is a daily intake of 3–4% of body weight on extruded feed. Our findings reveal that underfeeding led to diminished antioxidants and immune defenses in koi, while overfeeding also proved detrimental. An optimal feeding rate of 3–4% body weight per day is necessary to enhance growth and health in this species.
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Effects of Fermented Soybean Meal Replacing Fishmeal and Adding Lentinus edodes Fermentation Supplementation on Japanese Eel (Anguilla japonica) Cultivated in Pond Cage
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Jie Yu, Fei Xiao, Qian Xiao, Dongming Chen, Guoyong Zhou, Huanbin Jiang, Xiaoya Fan, Herong Shi and Huihong Zhao
Fishes 2025, 10(4), 180; https://doi.org/10.3390/fishes10040180 - 16 Apr 2025
Abstract
Replacing fishmeal (FM) with plant-based protein sources remains a significant challenge, particularly for carnivorous fish. This study investigates the effect of dietary Lentinus edodes fermentation (LEF) supplementation on Japanese eel (Anguilla japonica) fed with fermented soybean meal (FSM) as a partial
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Replacing fishmeal (FM) with plant-based protein sources remains a significant challenge, particularly for carnivorous fish. This study investigates the effect of dietary Lentinus edodes fermentation (LEF) supplementation on Japanese eel (Anguilla japonica) fed with fermented soybean meal (FSM) as a partial FM replacement. The positive control consisted of 64% FM (Con), and the negative control (FSM group) included 52% FM plus 12% FSM. Two experimental diets were formulated by adding 2% LEF (LEF2 group) and 3% LEF (LEF3 group) to the negative control diet. The experimental diet was administered to Japanese eels weighing 62.50 ± 2.14 g for 12 weeks. The experimental fish were randomly assigned to four groups, with three replicates of 100 fish per group. The results indicated that growth performance and feed efficiency were significantly reduced in the FSM group, but were significantly improved by LEF supplementation (p < 0.05). LEF supplementation did not significantly affect muscle crude fat and protein content compared to the FSM group (p > 0.05), but significantly increased muscle amino acid content and levels of certain fatty acids (linoleic acid, γ-linolenic acid, eicosatrienoic acid, DHA) (p < 0.05). LEF supplementation reduced serum TC and LDL-C levels, increased HDL-C levels, significantly increased CAT and T-SOD activities, and reduced MDA levels in both serum and liver (p < 0.05). ALT and AST activities were significantly elevated in the FSM group, accompanied by liver histological abnormalities, which were improved by LEF supplementation. LEF supplementation increased the thickness of the muscularis, villus height, and goblet cell count in the intestine (p < 0.05). Compared to the control, the FSM group significantly upregulated spleen tnf-α gene expression and downregulated the expression of anti-inflammatory factors (ifn-α, ifn-γ, socs1, mavs). LEF supplementation ameliorated the reduced immunocompetence induced by FM replacement with FSM by enhancing the expression of immune-related genes (irak4, ifn-α, ifn-γ, irf3, irf11, socs1, mavs, traf3) in the spleen. These results suggest that the beneficial effects of LEF supplementation on growth performance and feed efficiency may be attributed to its improvement of liver damage and intestinal histology, as well as its enhancement of antioxidant capacity and immunity.
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(This article belongs to the Special Issue Pivotal Roles of Feed Additives For Fish)
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Purification of Intensive Shrimp Farming Effluent by Gracilaria Coupled with Oysters
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Junjing Li, Yu Xu, Yunlong Deng, Xiaojuan Hu, Haochang Su, Guoliang Wen and Yucheng Cao
Fishes 2025, 10(4), 179; https://doi.org/10.3390/fishes10040179 - 15 Apr 2025
Abstract
In this study, we explored the ability of Gracilaria vermiculophylla coupled with Crassostrea hongkongensis to purify aquaculture effluent by analysing the purification of intensive shrimp farming effluent using G. vermiculophylla under different environmental conditions. After determining the optimal conditions, we further investigated the
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In this study, we explored the ability of Gracilaria vermiculophylla coupled with Crassostrea hongkongensis to purify aquaculture effluent by analysing the purification of intensive shrimp farming effluent using G. vermiculophylla under different environmental conditions. After determining the optimal conditions, we further investigated the capability of the G. vermiculophylla and oyster coupling in intensive shrimp farming effluent purification. The shrimp farming density was 200 individuals per cubic metre (equivalent to 0.2 individuals per litre). The optimal environmental parameters were as follows: oyster biomass of 4.5 kg·m−3, G. vermiculophylla biomass of 2 kg·m−3, water temperature of 25–30 °C, and salinity of 15–30‰; the total inorganic nitrogen, PO43−-P, total nitrogen, and total phosphorus removal rates were 59.56%, 97.43%, 63.67%, and 76.25%, respectively, with G. vermiculophylla increasing in weight by 31.01%. For every 1 kg increase in the dry weight of G. vermiculophylla, 36.89 g of N and 12.40 g of P could be absorbed from the effluent. Our findings indicate that the coupling of G. vermiculophylla with oysters greatly contributed to the purification of effluent from intensive shrimp farming and can, thus, be used for treating intensive shrimp farming effluent.
Full article
(This article belongs to the Special Issue Advances in Shrimp Aquaculture: Management and Sustainability)
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Long-Term Heavy Metal Bioaccumulation in Sprat (Sprattus sprattus) from the Romanian Black Sea: Ecological and Human Health Risks in the Context of Sustainable Fisheries
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Andra Oros and Madalina Galatchi
Fishes 2025, 10(4), 178; https://doi.org/10.3390/fishes10040178 - 15 Apr 2025
Abstract
This study evaluates the heavy metals concentrations in sprat (Sprattus sprattus, Linnaeus, 1758) from the Romanian Black Sea, assessing both ecological implications and human health risks associated with consumption. Using long-term data spanning 1994–2019, levels of copper (Cu), cadmium (Cd), lead
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This study evaluates the heavy metals concentrations in sprat (Sprattus sprattus, Linnaeus, 1758) from the Romanian Black Sea, assessing both ecological implications and human health risks associated with consumption. Using long-term data spanning 1994–2019, levels of copper (Cu), cadmium (Cd), lead (Pb), nickel (Ni), and chromium (Cr) in dorsal muscle tissues were analyzed to identify contamination trends and episodic pollution events. Although most concentrations remained below regulatory thresholds, occasional exceedances of Cd and Pb suggest intermittent pollution inputs. Health risks were assessed using dietary indices including estimated daily intake (EDI), target hazard quotient (THQ), total hazard quotient (TTHQ), and carcinogenic risk index (CRI). Findings indicate that, under current exposure levels, regular sprat consumption poses minimal risk. However, prolonged intake during peak contamination periods may contribute to cumulative toxic effects, with implications for ecosystem stability and food safety. Given the persistence of heavy metals and their interactions with co-occurring pollutants, such as persistent organic pollutants (POPs) and polycyclic aromatic hydrocarbons (PAHs), ongoing monitoring remains essential. This study supports the development of sustainable environmental policies aimed at protecting marine biodiversity and consumer health in the Black Sea region.
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(This article belongs to the Section Environment and Climate Change)
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Mitogenomic and Phylogenetic Analyses of Lysmata lipkei (Crustacea: Decapoda: Lysmatidae)
by
Xixi Zhang, Zhihuang Zhu, Jianxin Wang, Ge Shi and Qi Lin
Fishes 2025, 10(4), 177; https://doi.org/10.3390/fishes10040177 - 14 Apr 2025
Abstract
This study aims to elucidate the characteristics of the mitogenome of Lysmata lipkei and investigate its phylogenetic relationships. Using both the Illumina NovaSeq 6000 (Illumina, Inc., San Diego, CA, USA) and PacBio Sequel II (Pacific Biosciences of California, Inc., Menlo Park, CA, USA)
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This study aims to elucidate the characteristics of the mitogenome of Lysmata lipkei and investigate its phylogenetic relationships. Using both the Illumina NovaSeq 6000 (Illumina, Inc., San Diego, CA, USA) and PacBio Sequel II (Pacific Biosciences of California, Inc., Menlo Park, CA, USA) platforms, the complete mitogenome sequence of L. lipkei was determined. The mitogenome of L. lipkei was annotated, measuring 17,497 bp in length and comprising 13 protein-coding genes (PCGs), 2 ribosomal RNA genes (rRNAs), and 22 transfer RNA genes (tRNAs). The nucleotide composition of the genome exhibited an AT bias of 63.4%. Among the PCGs, the most frequently used codon was UUA. All tRNAs, except for trnD, which lacks the TψC loop, were capable of forming the typical cloverleaf structure. Phylogenetic trees for Caridea were constructed using Bayesian Inference (BI) and Maximum Likelihood (ML) methods based on the nucleotide sequences of the 13 PCGs. Both methods yielded consistent topological structures, with L. lipkei showing the closest phylogenetic relationship to L. kuekenthali. Additionally, Lysmatidae, Thoridae, and Hippolytidae formed a monophyletic clade. This research not only filled the gap in mitogenome data for Lysmatidae but also provided novel molecular insights into Caridean phylogenetics.
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(This article belongs to the Section Genetics and Biotechnology)
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Spatial–Temporal Variations in Fish Larvae and Juveniles in the Surf Zone Along the Coast of Jiangsu Province, China
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Xiaodong Wang, Yuying Chen, Boran Qin, Tianyu Zhang, Junsheng Zhong, Jinjin Shi and Chuqing Wang
Fishes 2025, 10(4), 176; https://doi.org/10.3390/fishes10040176 - 13 Apr 2025
Abstract
Surf zones are important nursery grounds for fish larvae and juveniles. However, little is known about fish larvae and juveniles in the surf zone along the coast of Jiangsu Province. To describe the species composition of fish larvae and juveniles, monthly collections were
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Surf zones are important nursery grounds for fish larvae and juveniles. However, little is known about fish larvae and juveniles in the surf zone along the coast of Jiangsu Province. To describe the species composition of fish larvae and juveniles, monthly collections were conducted at eight stations during the spring tide from February 2024 to January 2025. The fish larvae and juveniles were sampled using a seine net (1m × 4m; 1 mm mesh aperture), with sampling repeated three times at each station per month. A total of 1435 fish larvae and juveniles were collected, belonging to 42 species and 37 genera in 21 families. Almost half of them were postflexion larvae. Gobiidae, with 14 species, was the most diverse family. Based on the index of relative importance (IRI) result, the dominant species was Amoya pflaumi, accounting for 35.33% of the total number of individuals collected. Common species were Mugil cephalus, Amblychaeturichthys hexanema, Tridentiger trigonocephalus, Acanthogobius ommaturus, Mugilogobius abei, Thryssa mystax, Periophthalmus modestus, Sillago sihama, and Mugilogobius myxodermus. All other species were classified as rare. No fish larvae or juveniles were collected in February 2024 and January 2025. The species number, catch per unit effort (CPUE), Margalef’s richness index, Pielou’s evenness index, and Shannon–Wiener diversity index showed similar trends, and significant differences could be found for each parameter among sampling months. The cluster analysis from the ten months (excluding February 2024 and January 2025) indicated a significant seasonal change in the community structure of fish larvae and juveniles. Fish larvae and juveniles were abundant and diverse at one station near an aquaculture tidal flat for seaweed. This study provides essential basic data to support the management and conservation of fishery resources in the surf zone along the coast of Jiangsu Province.
Full article
(This article belongs to the Special Issue Biodiversity and Spatial Distribution of Fishes, Second Edition)
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Revealing the Structure and Biodiversity Patterns of Fish Communities in River Networks Based on Environmental DNA
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Ziyu Liu, Yongsheng Wu, Wenhui You, Shuxin Li, Ge Shi and Chen Zhang
Fishes 2025, 10(4), 175; https://doi.org/10.3390/fishes10040175 - 13 Apr 2025
Abstract
Revealing taxonomic, functional, and phylogenetic dimensions of biodiversity is critical for the effective conservation and management of aquatic organisms in freshwater ecosystems subjected to multiple threats. Fish biodiversity patterns in river ecosystems exhibit complex spatiotemporal variation influenced by hydrological connectivity and the dispersal
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Revealing taxonomic, functional, and phylogenetic dimensions of biodiversity is critical for the effective conservation and management of aquatic organisms in freshwater ecosystems subjected to multiple threats. Fish biodiversity patterns in river ecosystems exhibit complex spatiotemporal variation influenced by hydrological connectivity and the dispersal ability of species within the river network. This study utilized eDNA metabarcoding to investigate fish communities in three subtropical mountain rivers, aiming to uncover the community structure and spatiotemporal dynamics of the multidimensional biodiversity of fish communities across varying positions within the river network. The three biodiversity indices of the fish communities at the confluence were significantly greater than those of the mainstream community in the dry season, whereas the phylogenetic diversity of the fish community at the tributaries during the wet season was significantly greater than that in the mainstream. This likely reflected the unique spatial configuration of confluences and the seasonal dispersal of fish in the river network. The results of this study highlighted the important role of confluences and tributaries in river networks and the fact that they need to be prioritized to conserve riverine fish diversity.
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(This article belongs to the Special Issue Mapping and Monitoring Aquatic Biodiversity in Hotspot Habitat Areas Using Environmental DNA (eDNA))
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Open AccessArticle
Sub-Lethal Effects of Predators in Aquaculture: Assessment of Chronic Exposure to Conspecific Alarm Substance on Feeding and Growth Performances of Nile Tilapia
by
Rafaela Torres Pereira, Alexandre Luiz Arvigo, Caio Akira Miyai, Augusto Rysevas Silveira, Percília Cardoso Giaquinto, Helton Carlos Delicio, Leonardo José Gil Barcellos and Rodrigo Egydio Barreto
Fishes 2025, 10(4), 174; https://doi.org/10.3390/fishes10040174 - 12 Apr 2025
Abstract
In aquaculture practices, fish are mostly protected from lethal actions of predators. However, sub-lethal effects can be challenging to prevent, as they may be associated with chemical cues signaling predation risk that easily dissolve and spread in water, serving as potential stressors. These
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In aquaculture practices, fish are mostly protected from lethal actions of predators. However, sub-lethal effects can be challenging to prevent, as they may be associated with chemical cues signaling predation risk that easily dissolve and spread in water, serving as potential stressors. These cues originate from predators, stressed or injured prey releasing blood, a conspecific alarm substance (CAS), and/or other bodily fluids. In this study, we simulated a small-scale net cage system and assessed the feeding and growth of Nile tilapia exposed chronically to a CAS. Nile tilapia, an invasive species in many aquatic systems, frequently coexist freely alongside those cultivated in cages. Consequently, caged tilapia may regularly be exposed to a CAS, potentially leading to chronic stress and impacting growth and development. Fish were exposed daily to either a CAS or a control vehicle (distilled water) for 45 days (one fish per cage). Fish in both conditions exhibited similar increases in body mass, weight gain, and length over time and displayed an allometric negative growth profile, indicating that the CAS did not affect the length–weight relationship as well. Specific and relative growth rates, condition factor, body axes, food intake, and feeding conversion efficiency were also unaffected by the CAS over time. This body of evidence suggests that the CAS did not act as a chronic stressor for caged Nile tilapia and a possible explanation is habituation.
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(This article belongs to the Special Issue Stress Physiology in Aquatic Animals)
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Open AccessArticle
UM-YOLOv10: Underwater Object Detection Algorithm for Marine Environment Based on YOLOv10 Model
by
Rengui Mai and Ji Wang
Fishes 2025, 10(4), 173; https://doi.org/10.3390/fishes10040173 - 11 Apr 2025
Abstract
In order to address the challenges of a low detection accuracy, missed detections, and false detections in marine precious biological target detection within complex marine environments, this paper presents a novel residual attention module called R-AM. This module is integrated into the backbone
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In order to address the challenges of a low detection accuracy, missed detections, and false detections in marine precious biological target detection within complex marine environments, this paper presents a novel residual attention module called R-AM. This module is integrated into the backbone network of the YOLOv10 model to improve the model’s focus on the detailed features of biological targets during feature extraction. Additionally, the introduction of a bidirectional feature pyramid with adaptive feature fusion in the neck network enhances the integration of semantic information from deep layers, and localization cues from shallow layers improve the model’s ability to distinguish targets from their environments. The experimental data showed that the improved YOLOv10 model achieved 92.89% at mAP@0.5, increasing by 1.31% compared to the original YOLOv10 model. Additionally, the mAP@0.5:0.95 was 77.13%, indicating a 3.71% improvement over the original YOLOv10 model. When compared to the Faster R-CNN, SSD, RetinaNet, YOLOv6, and YOLOv7 models, the enhanced model exhibited increases of 1.5%, 1.7%, 4.06%, 4.7%, and 1.42% in mAP@0.5, respectively. This demonstrates a high detection accuracy and robust stability in complex seabed environments, providing valuable technical support for the scientific management of marine resources in underwater ranches.
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(This article belongs to the Section Fishery Facilities, Equipment, and Information Technology)
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Open AccessArticle
Study on the Flow Velocity Preference of the Four Major Chinese Carps Using Convolutional Neural Networks
by
Ning Qiu, Wenjing Li, Yi Yu, Jianna Jia, Guoqiang Ma and Shitao Peng
Fishes 2025, 10(4), 172; https://doi.org/10.3390/fishes10040172 - 11 Apr 2025
Abstract
Flow velocity is a critical factor in determining the suitability of fish habitats. Understanding the preference patterns of the four major Chinese carps (FMCCs) for different flow velocities is crucial for their habitat conservation and restoration. In this study, the preference of individual
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Flow velocity is a critical factor in determining the suitability of fish habitats. Understanding the preference patterns of the four major Chinese carps (FMCCs) for different flow velocities is crucial for their habitat conservation and restoration. In this study, the preference of individual fish species, approximately 15 cm in length, for flow velocity was investigated at flow velocity gradients of 0.0, 0.4, 0.8, 1.2, 1.6, and 2.0 times their body length. Additionally, a deep learning algorithm based on convolutional neural networks (CNNs) was employed for fish target detection. The results showed that, at this length, black carp (Mylopharyngodon piceus) preferred fast currents when the inlet flow velocity was between 0.4 and 1.6 times their body length, while grass carp (Ctenopharyngodon idella), silver carp (Hypophthalmichthys molitrix), and bighead carp (Hypophthalmichthys nobilis) preferred fast currents when the inlet flow velocity of the test flume was between 0.4 and 2.0 times their body length. However, this preference for fast currents decreased as the overall flow velocity increased to a specific threshold, eventually leading to their avoidance. The highest preference for fast currents among the four species was observed at inlet flow velocities of 1.2, 0.4, 0.8, and 0.8 times their body length, respectively. The findings of this study provide important insights into habitat conservation and restoration for the FMCCs in projects focused on the construction of navigation channels and water conservancy.
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(This article belongs to the Topic The Importance of Fish Phenotype in Aquaculture, Fisheries and Conservation)
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Open AccessArticle
Effects of Stocking Densities on the Growth Performance and Physiology of Juvenile Piaractus brachypomus in Recirculating Aquaculture System
by
Imaculada de Morais Carvalho Ananias, Sidney dos Santos Silva, André de Sena Souza, Fábio Aremil Costa dos Santos, Thamara Bentivole Magalhães, Wagner Junio de Freitas Martins, Gisele Cristina Favero and Ronald Kennedy Luz
Fishes 2025, 10(4), 171; https://doi.org/10.3390/fishes10040171 - 11 Apr 2025
Abstract
The effects of different stocking densities on the growth and physiology of juvenile Piaractus brachypomus were evaluated in two experiments. Experiment I used juveniles weighing 1.5 ± 0.4 g at the following densities for 20 days: D0.68—0.68 kg/m3; D
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The effects of different stocking densities on the growth and physiology of juvenile Piaractus brachypomus were evaluated in two experiments. Experiment I used juveniles weighing 1.5 ± 0.4 g at the following densities for 20 days: D0.68—0.68 kg/m3; D1.45—1.45 kg/m3; D4.41—4.41 kg/m3; and D7.17—7.17 kg/m3. Experiment II used juveniles weighing 6.66 ± 1.3 g at the following densities for 20 days: D1.0—1.00 kg/m3; D1.95—1.95 kg/m3; D5.63—5.63 kg/m3, and D7.90—7.90 kg/m3. Both experiments showed a reduction in dissolved oxygen levels in the water, with Experiment II showing a plateau effect from 2.34 kg/m3 (p < 0.05). Final weight, final length, weight gain, daily weight gain, and specific growth rate were inversely proportional to density (p < 0.05), while final biomass, feed intake, and feed conversion were directly related to density in both experiments (p < 0.05). At the end of Experiment II, plasma triglycerides decreased as stocking density increased (p < 0.05), and hemoglobin and mean corpuscular volume were higher at the lowest density (D1.0) (p < 0.05). High stocking densities reduced dissolved oxygen, characterizing a hypoxic state in both experiments, affecting growth and some physiological parameters. Therefore, studies testing stocking densities for P. brachypomus in normoxic situations are still needed.
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(This article belongs to the Section Fishery Economics, Policy, and Management)
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Open AccessArticle
Impact of Faba Bean (Vicia faba L.) Diet and Subsequent Withdrawal on GIFT Tilapia (Oreochromis niloticus) Muscle Quality
by
Guanling Xu, Wei Xing, Tieliang Li, Shibo Wei, Ying Zhang, Tingting Song, Huanhuan Yu and Lin Luo
Fishes 2025, 10(4), 170; https://doi.org/10.3390/fishes10040170 - 11 Apr 2025
Abstract
To systematically evaluate FB’s effects on tilapia muscle quality, two distinct experimental phases are designed, the crispy texture development phase (0–16 weeks) and the crispy texture retention phase (17–24 weeks), which can determine the minimum faba bean (FB) feeding duration required to achieve
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To systematically evaluate FB’s effects on tilapia muscle quality, two distinct experimental phases are designed, the crispy texture development phase (0–16 weeks) and the crispy texture retention phase (17–24 weeks), which can determine the minimum faba bean (FB) feeding duration required to achieve optimal textural modification and can assess the persistence of improved textural properties following FB withdrawal, respectively. The results demonstrated that a 60% FB inclusion diet administered for 16 weeks did not adversely affect tilapia growth performance. Significant improvements in textural parameters, including hardness, springiness, chewiness, and shear force, were observed in FB-fed tilapia as early as 8 weeks, with these enhancements being maintained throughout the 16-week feeding period. These superior textural characteristics persisted during the subsequent retention phase following FB withdrawal. Microstructural analysis revealed that the 60% FB diet significantly enhanced muscle-fiber density while reducing fiber diameter in tilapia during the 8–16 week feeding period. These microstructural modifications persisted throughout the texture retention phase, maintaining significant differences compared to the control group. Serological analysis demonstrated the FB group elevated Superoxide dismutase (SOD) activity and reduced malondialdehyde (MDA) levels at 4 weeks, though these differences normalized thereafter. qRT-PCR showed the 60% FB clearly increased the expression of mstn at 8 weeks, while col1a-2 and myog expressions also obviously improved at 12 weeks. In summary, dietary 60% FB improved tilapia muscle crispiness by altering texture and microstructure via gene-expression regulation. The minimum duration was 8 weeks to achieve crispiness in tilapia by the 60% FB diet without adverse effects on growth, immunity, and hepatopancreas function. Furthermore, the crispy texture of tilapia fillets was maintained for at least 2 months following withdrawal of the 60% FB diet after 16 weeks of continuous feeding.
Full article
(This article belongs to the Special Issue Growth, Metabolism, and Flesh Quality in Aquaculture Nutrition)
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Open AccessArticle
Using Baited Remote Underwater Video Surveys (BRUVs) to Analyze the Structure of Predators in Guanahacabibes National Park, Cuba
by
Dorka Cobián-Rojas, Jorge Angulo-Valdés, Pedro Pablo Chevalier-Monteagudo, Lázaro Valentín García-López, Susana Perera-Valderrama, Joán Irán Hernández-Albernas and Hansel Caballero-Aragón
Fishes 2025, 10(4), 169; https://doi.org/10.3390/fishes10040169 - 10 Apr 2025
Abstract
The reef fish communities of the Guanahacabibes National Park have been studied for 20 years using various methodologies that have allowed us to understand aspects of their diversity and structure. However, due to gaps in information about the abundance and distribution of mesopredators
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The reef fish communities of the Guanahacabibes National Park have been studied for 20 years using various methodologies that have allowed us to understand aspects of their diversity and structure. However, due to gaps in information about the abundance and distribution of mesopredators (big fish and sharks), a new study was conducted in 2017 to determine their structure, explore the influence of different factors on their spatial variability, and evaluate their behavior. To achieve this, the Baited Remote Underwater Video Surveys (BRUVs) methodology was successfully applied, locating a single set of BRUVs at 90 sites distributed across 9 sectors of the park’s functional zoning. Variability in mesopredator metrics and their potential prey was assessed through a PERMANOVA analysis; a distance-based linear model (DISTLM) was used to explore the relationship between mesopredator abundance and biological, abiotic, and condition variables; and animal behavior was classified as incidental, cautious, or aggressive. A total of 64 fish species were identified, 7 of which were mesopredators, and 3 were sharks. An uneven distribution and abundance were observed among sectors, with the most abundant mesopredators being Carcharhinus perezi, Sphyraena barracuda, and Mycteroperca bonaci. Mesopredator abundance was more closely related to the condition of zone use and its regulations than to biological and abiotic variables. Sharks were more abundant in strictly protected areas, which coincided with relatively murky waters and stronger currents. More than 50% of the observed sharks displayed exploratory and aggressive behavior towards the bait basket. The analyzed metrics validate the effectiveness of the management of the protected area and suggest the presence of healthy and resilient mesopredator fish communities.
Full article
(This article belongs to the Special Issue Movement Ecology and Conservation of Large Marine Fishes (and Sharks))
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