Enhanced Cell Inactivation and Double-Strand Break Induction in V79 Chinese Hamster Cells by Monochromatic X-Rays at Phosphorus K-Shell Absorption Peak
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Line and Culture Media
2.2. Irradiation Setup
2.3. Colony Formation Assay for Survival Curves
2.4. Pulse Field Gel Electrophoresis (PFGE) and DSB Measurements
3. Results
3.1. Cell Inactivation on and Below the Phosphorous K-Shell Peak
3.2. Induction of DSBs
3.3. Cross-Section of DSB Induction and Quantum Yield
4. Discussion
4.1. Evaluation of Enhanced Cell Inactivation on Phosphorus K-Shell Peak from Linear-Quadratic Model
4.2. Enhance Induction of DNA DSBs
4.3. Conversion of Exposure ψ (C/kg) to Absorbed Dose D (Gy): Mass Energy-Absorption Coefficient μen/ρ
4.4. Cell Inactivation and DSB Induction per Absorbed Dose in Gy
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Materials | μen/ρ [m2/kg] | |
---|---|---|
2.147 keV | 2.153 keV | |
Air (1) | 42.79 | 42.45 |
Water (1) | 50.17 | 49.77 |
Soft tissue (2) | 45.66 | 48.75 |
Cell nucleus (2) | 46.11 | 71.05 |
DNA (3) | 41.8 | 140.4 |
Energy [keV] | α [C/kg]−1 | β [C/kg]−2 | Ψ37 [C/kg] | Ψ10 [C/kg] | Ψ1 [C/kg] |
---|---|---|---|---|---|
2.147 | 5.54 ± 1.7 | 113.5 ± 11.9 | 0.0723 | 0.120 | 0.179 |
2.153 | 14.4 ± 2.5 | 124.2 ± 19.2 | 0.0486 | 0.090 | 0.143 |
f1 | 2.60 ± 0.93 | 1.09 ± 0.2 | 1.49 | 1.33 | 1.25 |
Energy [keV] | α [Gy]−1 | β [Gy]−2 | D37 [Gy] | D10 [Gy] | D1 [Gy] |
---|---|---|---|---|---|
2.147 | 0.152 ± 0.047 | 0.085 ± 0.009 | 2.64 | 4.38 | 6.51 |
2.153 | 0.254 ± 0.045 | 0.039 ± 0.006 | 2.75 | 5.10 | 8.11 |
f1 | 1.67 ± 0.59 | 0.45 ± 0.07 | 0.96 | 0.86 | 0.80 |
Energy [keV] | R DSB/Mbp /Ψ | R′ 10−3 DSB/Mbp /Gy | R″ DSB/Cell /Gy | DSB/Cell/Ψi (or Di) | ||
---|---|---|---|---|---|---|
Ψ37 (D37) | Ψ10 (D10) | Ψ1 (D1) | ||||
2.147 | 0.224 ± 0.015 | 6.10 ± 0.40 | 31.4 ± 2.1 | 20.0 | 46.3 | 90.7 |
2.153 | 0.350 ± 0.020 | 6.20 ± 0.40 | 31.9 ± 2.1 | 22.1 | 61.5 | 135.8 |
f1 | 1.56 ± 0.13 | 1.02 ± 0.26 | 1.02 ± 0.26 | 1.10 | 1.33 | 1.50 |
Energy [keV] | Cross-Section 10−13 [m2] | Quantum Yield *** | |
---|---|---|---|
σDSB | σabsorption * | ||
2.147 | 5.02 ± 0.33 | 2.42 ± 0.46 | - |
2.153 | 7.79 ± 0.33 | 8.13 ± 0.16 | - |
phosphorus K-shell ** | 2.77 ± 0.55 | 5.71 ± 0.49 | 0.49 ± 0.18 # |
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Konishi, T.; Yoshihara, D.; Maeda, M.; Ohsawa, D.; Usami, N.; Hieda, K. Enhanced Cell Inactivation and Double-Strand Break Induction in V79 Chinese Hamster Cells by Monochromatic X-Rays at Phosphorus K-Shell Absorption Peak. Quantum Beam Sci. 2020, 4, 38. https://doi.org/10.3390/qubs4040038
Konishi T, Yoshihara D, Maeda M, Ohsawa D, Usami N, Hieda K. Enhanced Cell Inactivation and Double-Strand Break Induction in V79 Chinese Hamster Cells by Monochromatic X-Rays at Phosphorus K-Shell Absorption Peak. Quantum Beam Science. 2020; 4(4):38. https://doi.org/10.3390/qubs4040038
Chicago/Turabian StyleKonishi, Teruaki, Daichi Yoshihara, Munetoshi Maeda, Daisuke Ohsawa, Noriko Usami, and Kotaro Hieda. 2020. "Enhanced Cell Inactivation and Double-Strand Break Induction in V79 Chinese Hamster Cells by Monochromatic X-Rays at Phosphorus K-Shell Absorption Peak" Quantum Beam Science 4, no. 4: 38. https://doi.org/10.3390/qubs4040038