PHB Production in Biofermentors Assisted through Biosensor Applications †
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganisms and Plasmid Preparation for E. coli Transformation
2.2. Culture Media and Conditions
2.3. Amplification and Cloning of phaCAB Genes in E. coli BL21(DE3)
2.4. Analytical Procedures during Biofermentor Use
2.4.1. Quantification of Reducing Sugars
2.4.2. Cell Dry Weight (CDW)
2.4.3. Monitoring the Production of PHB by Nile Blue Staining
2.4.4. PHB/PHA Quantification and Extraction
3. Results and Discussion
4. Conclusions
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
PHA | poylhydroxyalanoate |
PHB | polyhydroxybutyrate |
PHB-V | mixed type PHA, containing hydroxyvalerate |
EIS | electrochemical impedance spectroscopy |
FTIR | Fourier Transformation-IR Spectrometry |
SERS | Surface Enhanced Rama Spectroscopy |
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Composition | Sweet Corn (g/L) | Potato Skin (g/L) | Banana Juice (g/L) |
---|---|---|---|
Glucose | 104 | 45 | 90 |
Fructose | 10 | ≤5 | 82 |
Maltose | ≤5 | 10 | nd |
Sucrose | ≤5 | ≤5 | nd |
Nitrogen | 3.6 | 4 | 6 |
Citric acid | nd | nd | 3.5 |
Magnesium | nd | nd | 0.3 |
Phosphate | nd | nd | 0.25 |
Fed-Batch Hydrolysate | CDW(g L−1) | PHB (g L−1) |
---|---|---|
Sweet Corn Enzymatic hydrolysate 10%: | ||
Recombinant BL21(DE3) E.coli | 8.2 | 1.7 |
Empty BL21(DE3) E.coli | 7.9 | / |
Ralstonia taiwanensis | 4.3 | / |
Potato Skin Enzymatic hydrolysate 25%: | ||
Recombinant BL21(DE3)E. coli | 15.3 | 2.9 |
Empty BL21(DE3) E. coli | 14.9 | / |
Ralstonia taiwanensis | 9.5 | 0.4 |
Banana juice 5% hydrolysate: | ||
Recombinant BL21(DE3) E. coli | 20.6 | 3.9 |
Empty BL21(DE3) E. coli | 20.2 | / |
Ralstonia taiwanensis | 15.8 | 0.2 |
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Poltronieri, P.; Mezzolla, V.; D’Urso, O.F. PHB Production in Biofermentors Assisted through Biosensor Applications. Proceedings 2017, 1, 4. https://doi.org/10.3390/ecsa-3-E014
Poltronieri P, Mezzolla V, D’Urso OF. PHB Production in Biofermentors Assisted through Biosensor Applications. Proceedings. 2017; 1(2):4. https://doi.org/10.3390/ecsa-3-E014
Chicago/Turabian StylePoltronieri, Palmiro, Valeria Mezzolla, and Oscar Fernando D’Urso. 2017. "PHB Production in Biofermentors Assisted through Biosensor Applications" Proceedings 1, no. 2: 4. https://doi.org/10.3390/ecsa-3-E014