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Abstract

Electrochemical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz Multielectrode Array Biosensors †

by
Rosalía González-Brito
1,*,
Pablo Montenegro
1,
Alicia Méndez
1,
Ramtin E. Shabgahi
2,
Alberto Pasquarelli
2 and
Ricardo Borges
1,*
1
Pharmacology Unit, Medical School, Universidad de La Laguna, 38200 La Laguna, Spain
2
Institute of Electron Devices and Circuits, Ulm University, 89081 Ulm, Germany
*
Authors to whom correspondence should be addressed.
†
Presented at the 4th International Electronic Conference on Biosensors, 20–22 May 2024; Available online: https://sciforum.net/event/IECB2024.
Proceedings 2024, 104(1), 18; https://doi.org/10.3390/proceedings2024104018
Published: 28 May 2024
(This article belongs to the Proceedings of The 4th International Electronic Conference on Biosensors)
Versions Notes

1. Introduction

About 90% of blood serotonin is stored in secretory granules of platelets and is released by exocytosis. Serotonin is a biogenic amine and the mechanisms for its accumulation in secretory granules and exocytosis are similar to some neurotransmitters. As it is an electroactive molecule, its release can be detected via amperometry. Thus, platelets are an easily accessible human cell model to study exocytosis.

2. Methods

We have optimized boron-doped diamond (BDD) on multielectrode array (MEA) systems that allow the detection of amperometric recordings from the quantum release of serotonin from human platelets. Exocytotic events are detected as transient oxidation currents. Our initial experiments were carried out with microelectrode devices on silicone substrate (BDD-on-silicon MEAs) [1]. We introduce here a new transparent material which allows microscopy observations: a BDD-on-quartz MEA.

3. Results

BDD-on-quartz MEA devices exhibit excellent electrochemical properties similar to BDD-on-silicon MEAs [1]. We present the amperometric data obtained from unloaded platelets and after loading the platelets with 10 µM serotonin for 2 h, as well as a comparative study of the quantum and kinetic characteristics of amperometric spikes obtained with both MEA chips.

4. Conclusions

We demonstrate the effectiveness of BDD-on-quartz MEAs as biosensors for the amperometric measurement of serotonin exocytosis from human platelets.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/proceedings2024104018/s1. Conference presentation.

Author Contributions

Conceptualization, R.B., and A.P.; methodology, R.G.-B., P.M., and A.M.; formal analysis, R.G.-B., P.M., and A.M.; software and validation, R.E.S.; review and editing, R.G.-B., R.B., and A.P. All authors have read and agreed to the published version of the manuscript.

Funding

This work was partially supported by the Spanish Ministry of Ciencia e Innovación, grant No. PID2020-116589GB-I00, to R.B.

Institutional Review Board Statement

This study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethical Committee at the University of La Laguna (CEIBA2017-0244) and by the Ethical Committee at the Canary Islands Health Services (CHUC_2020_80) for the use of human samples.

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

The data presented in this study are available in the references.

Conflicts of Interest

The authors declare no conflict of interest.

Reference

  1. González Brito, R.; Montenegro, P.; Méndez, A.; Carabelli, V.; Tomagra, G.; Shabgahi, R.E.; Pasquarelli, A.; Borges, R. Multielectrode Arrays as a Means to Study Exocytosis in Human Platelets. Biosensors 2023, 13, 86. [Google Scholar] [CrossRef] [PubMed]
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

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MDPI and ACS Style

González-Brito, R.; Montenegro, P.; Méndez, A.; Shabgahi, R.E.; Pasquarelli, A.; Borges, R. Electrochemical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz Multielectrode Array Biosensors. Proceedings 2024, 104, 18. https://doi.org/10.3390/proceedings2024104018

AMA Style

González-Brito R, Montenegro P, Méndez A, Shabgahi RE, Pasquarelli A, Borges R. Electrochemical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz Multielectrode Array Biosensors. Proceedings. 2024; 104(1):18. https://doi.org/10.3390/proceedings2024104018

Chicago/Turabian Style

González-Brito, Rosalía, Pablo Montenegro, Alicia Méndez, Ramtin E. Shabgahi, Alberto Pasquarelli, and Ricardo Borges. 2024. "Electrochemical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz Multielectrode Array Biosensors" Proceedings 104, no. 1: 18. https://doi.org/10.3390/proceedings2024104018

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