A Green Approach for Isolation of Phytochemicals from Lamiaceae Plants †
by
,
Branislava Teofilović
1,*
,
Nevena Grujić-Letić
1,
Emilia Gligorić
1,
Snežana Papović
2,
Žarko Gagić
3,
Biljana Tubić
3 and
Milan Vraneš
2 1
Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia
2
Faculty of Sciences, University of Novi Sad, 21000 Novi Sad, Serbia
3
Faculty of Medicine, University of Banja Luka, 78000 Banja Luka, Bosnia and Herzegovina
*
Author to whom correspondence should be addressed.
†
Presented at the 14th European Nutrition Conference FENS 2023, Belgrade, Serbia, 14–17 November 2023.
Proceedings 2023, 91(1), 224; https://doi.org/10.3390/proceedings2023091224
Published: 4 February 2024
(This article belongs to the Proceedings of The 14th European Nutrition Conference FENS 2023)
Abstract
:Deep eutectic solvents (DESs) are now widely recognized as a new class of ionic liquid analogues and have large-scale usage as green solvents. Due to their superior characteristics compared to traditional organic solvents, DESs are increasingly being used. The aim of the work was to examine the effectiveness of DES extractants of phenolic compounds from monocomponent teas from plants of the Lamiaceae family produced at the Institute for the Study of Medicinal Plants, “Dr. Josif Pančić”. Ten samples of herbal drugs (basil, thyme, savory, oregano, sage, thyme, mint, lavender, rosemary, and lemon balm) were tested. Extraction was performed with two types of DESs (a mixture of menthol and methylsalicylate (1:1) and a mixture of menthol and dodecanoic acid (2:1)), as well as microwave and traditional extraction. The content of phenolic acids and flavonoids in the tested preparations was determined using high-performance liquid chromatography (HPLC). Menthol–methylsalicylate and menthol–dodecanoic acid mixtures showed significant efficiency in the isolation of quercetin and naringenin from herbal drugs, while the effectiveness of the menthol–methylsalicylate mixture stands out in the isolation of chlorogenic acid and cinnamic acid from all samples, as well as rosmarinic acid from basil. The concentration of quercetin ranged from 0.2035 to 0.7543 mg/g of the drug for the menthol–methylsalicylate mixture, and from 0.1189 to 0.2025 mg/g of the drug for the menthol–dodecanoic acid mixture. In this study, an environmentally friendly, economical, and efficient extraction based on menthol and methylsalicylate, and also menthol and dodecanoic acid, was applied. Based on the results presented, DESs may be useful in the isolation of target compounds from plants. This research represents an excellent basis for further research and testing of other green solvents as new extractants.
Author Contributions
Conceptualization, B.T. (Branislava Teofilović) and N.G.-L.; methodology, B.T. (Branislava Teofilović); software, E.G.; validation, M.V., S.P. and Ž.G.; formal analysis, B.T. (Biljana Tubić); investigation, B.T. (Branislava Teofilović); resources, B.T. (Branislava Teofilović); data curation, E.G.; writing—original draft preparation, B.T. (Branislava Teofilović); writing—review and editing, N.G.-L.; visualization, S.P.; supervision, M.V.; project administration, M.V.; funding acquisition, N.G.-L. All authors have read and agreed to the published version of the manuscript.
Funding
This work is supported by Provincial Secretariat for Higher Education and Scientific Research, Province of Vojvodina (Grant No. 142-451-247/2023-01 and 142-451-2545/2021).
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
The data presented in this study are available on request from the corresponding author.
Conflicts of Interest
The authors declare no conflicts of interest.
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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Teofilović, B.; Grujić-Letić, N.; Gligorić, E.; Papović, S.; Gagić, Ž.; Tubić, B.; Vraneš, M. A Green Approach for Isolation of Phytochemicals from Lamiaceae Plants. Proceedings 2023, 91, 224. https://doi.org/10.3390/proceedings2023091224
AMA Style
Teofilović B, Grujić-Letić N, Gligorić E, Papović S, Gagić Ž, Tubić B, Vraneš M. A Green Approach for Isolation of Phytochemicals from Lamiaceae Plants. Proceedings. 2023; 91(1):224. https://doi.org/10.3390/proceedings2023091224
Chicago/Turabian StyleTeofilović, Branislava, Nevena Grujić-Letić, Emilia Gligorić, Snežana Papović, Žarko Gagić, Biljana Tubić, and Milan Vraneš. 2023. "A Green Approach for Isolation of Phytochemicals from Lamiaceae Plants" Proceedings 91, no. 1: 224. https://doi.org/10.3390/proceedings2023091224
