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Volume 79
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Advances in Respiratory Medicine is published by MDPI from Volume 90 Issue 4 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Via Medica.
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Article

Interferon-Gamma Assay T-SPOT.TB in the Diagnostics of Latent Tuberculosis Infection

by
Dagmara Borkowska
1,
Zofia Zwolska
1,
Dorota Michałowska-Mitczuk
2,
Maria Korzeniewska-Koseła
3,
Anna Zabost
1,
Agnieszka Napiórkowska
1,
Monika Kozińska
1,
Sylwia Brzezińska
1 and
Ewa Augustynowicz-Kopeć
1,*
1
Department of Microbiology, Institute of Tuberculosis and Lung Diseases in Warsaw, Płocka 26, 01-138 Warsaw, Poland
2
Outpatient Clinic, Institute of Tuberculosis and Lung Diseases in Warsaw, 01-138 Warsaw, Poland
3
Department of Epidemiology and Organisation of Fight Against Tuberculosis, Institute of Tuberculosis and Lung Diseases in Warsaw, 01-138 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Adv. Respir. Med. 2011, 79(4), 264-271; https://doi.org/10.5603/ARM.27644
Submission received: 18 November 2010 / Revised: 15 June 2011 / Accepted: 15 June 2011 / Published: 15 June 2011

Abstract

Introduction: Diagnostics of latent tuberculosis infection (LTBI) has been based on a century-old tuberculin skin test (TST). However, a positive reaction can result not only from infection with Mycobacterium tuberculosis, but also from BCG vaccination or cross-reaction with nontuberculous mycobacteria. T-SPOT.TB assay is a new test to diagnose tuberculosis infection by measuring in vitro T-cell interferon-gamma release in response to two Mycobacterium tuberculosis-specific antigens: ESAT-6 and CFP-10. Material and methods: T-SPOT.TB assay was performed on samples of whole blood (n = 137) from March to September 2010. Tuberculin skin test was carried out in 96 participants. A positive TST result was considered to be an induration of 10 mm or more. Results: Of the 137 patients tested, T-SPOT.TB assay results were positive in 37 (27%), negative in 98 (71.5%) and indeterminate in only 2 (1.5%) persons. We analyzed T-SPOT.TB and TST results in 96 patients who were subjected to both tests. Concordance between T-SPOT.TB and TST results (a 10-mm skin reaction interpreted as positive) was 79%. Fifteen (15.6%) patients had a positive TST result and a negative T-SPOT.TB, and 5 (5.2%) patients had a negative TST result and a positive T-SPOT.TB. We observed a good correlation between positive T-SPOT.TB results and the diameter of induration of ≥15 mm in TST results. Conclusions: T-SPOT.TB offers a more accurate approach than TST in the identification of tuberculosis infection. The study showed that the test T-SPOT.TB is a good diagnostic tool in identifying persons with tuberculosis infection. For a full confirmation of this assessment, it is necessary to examine more cases.
Keywords: latent tuberculosis infection; IGRA tests; tuberculin skin test; interferon-γ; antigens ESAT-6 and CFP-10 latent tuberculosis infection; IGRA tests; tuberculin skin test; interferon-γ; antigens ESAT-6 and CFP-10

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MDPI and ACS Style

Borkowska, D.; Zwolska, Z.; Michałowska-Mitczuk, D.; Korzeniewska-Koseła, M.; Zabost, A.; Napiórkowska, A.; Kozińska, M.; Brzezińska, S.; Augustynowicz-Kopeć, E. Interferon-Gamma Assay T-SPOT.TB in the Diagnostics of Latent Tuberculosis Infection. Adv. Respir. Med. 2011, 79, 264-271. https://doi.org/10.5603/ARM.27644

AMA Style

Borkowska D, Zwolska Z, Michałowska-Mitczuk D, Korzeniewska-Koseła M, Zabost A, Napiórkowska A, Kozińska M, Brzezińska S, Augustynowicz-Kopeć E. Interferon-Gamma Assay T-SPOT.TB in the Diagnostics of Latent Tuberculosis Infection. Advances in Respiratory Medicine. 2011; 79(4):264-271. https://doi.org/10.5603/ARM.27644

Chicago/Turabian Style

Borkowska, Dagmara, Zofia Zwolska, Dorota Michałowska-Mitczuk, Maria Korzeniewska-Koseła, Anna Zabost, Agnieszka Napiórkowska, Monika Kozińska, Sylwia Brzezińska, and Ewa Augustynowicz-Kopeć. 2011. "Interferon-Gamma Assay T-SPOT.TB in the Diagnostics of Latent Tuberculosis Infection" Advances in Respiratory Medicine 79, no. 4: 264-271. https://doi.org/10.5603/ARM.27644

APA Style

Borkowska, D., Zwolska, Z., Michałowska-Mitczuk, D., Korzeniewska-Koseła, M., Zabost, A., Napiórkowska, A., Kozińska, M., Brzezińska, S., & Augustynowicz-Kopeć, E. (2011). Interferon-Gamma Assay T-SPOT.TB in the Diagnostics of Latent Tuberculosis Infection. Advances in Respiratory Medicine, 79(4), 264-271. https://doi.org/10.5603/ARM.27644

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