Investigating Forkhead Box O Transcription Factor 1 Gene’s Relation to Immunoglobulin E in House Dust Mite-Allergic Asthma Patients
Abstract
:Highlights
- Subcutaneous immunotherapy is a long-term effective immunotherapy;
- Immunity biomarkers such as IgE, FoxO1, and Sirtuin 1 are important biomarkers that have potential roles in the pathogenesis of asthma and remission of clinical symptoms.
- There is correlation between IgE, FoxO1, and Sirtuin 1;
- Further broader-scale studies are needed to determine a novel protocol for the control and remission of clinical symptoms of HDM-allergic asthma.
Abstract
1. Introduction
2. Patients and Method
2.1. Study Design and Ethical Considerations
2.1.1. Sample Size
2.1.2. Inclusion and Exclusion Criteria
2.1.3. Assessment of Severity of Asthma
2.1.4. Pulmonary Function Test
2.1.5. Skin Prick Test
2.2. Samples
2.3. Measurement of the mRNA Expression Levels of FoxO1 and Sirtuin 1
2.3.1. Separation of Peripheral Blood Mononuclear Cells (PBMCs)
2.3.2. RNA Extraction and Isolation
2.3.3. Synthesis of the First-Strand Complementary DNA
2.3.4. Quantitative Analysis of FoxO1, SIRT1, and 18S rRNA Genes
2.4. Enzyme-Linked Immunosorbent Assay
2.5. Statistical Analysis
3. Results
Participants Criteria
4. Discussion
5. Limitations
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer | Sense Primer Sequence | Antisense Primer | Product Size (bp) |
---|---|---|---|
FoxO1 | 5′-GTCAAGAGCGTGCCCTACTTCA-3′ | 5′-TGAACTTGCTGTGTAGGGACAGATTAT-3′ | 102 [22] |
SIRT1 | 5′-TGCTGGCCTAATAGAGTGGCA-3′ | 5′-CTCAGCGCCATGGAAAATGT-3′ | 101 [23] |
18S rRNA | 5′-AGT CCC TGC CCT TTG TAC ACA-3′ | 5′-GAT CCG AGG GCC TCA CTA AAC-3′ | 69 [24] |
PCR Step | Temperature | Time | Repeat Cycles |
---|---|---|---|
Initial denaturation | 95 °C | 10 min | |
Denaturation | 95 °C | 15 s | 40 |
Annealing | 60 °C | 30 s | |
Extension | 72 °C | 30 s | 30 |
Criteria | HV n = 40 | HDM/AA n = 40 | HDM/SCIT n = 40 | F | p Value |
---|---|---|---|---|---|
AGE Mean ± SD | 30.75 ± 9.8 | 29.60 ± 11.5 | 34.08 ± 12.3 | 0.337 | ns $ |
Gender male/female | 17/23 (42.5/57.5%) | 18/22 (45/55%) | 20/20 (50/50%) | - | |
Residence Urban Rural | 25 (62%) 15 (37.5%) | 22 (55%) 18 (45%) | 23 (57.5%) 17 (42.5%) | - | |
Family history of asthma Negative Positive | 31 (77.5%) 9 (22.5%) | 8 (20%) 32 (80%) | 9 (22.5%) 31 (77.5%) | - | |
Skin prick test | 0% | 100% | 100% | - | |
House dust mites D. pteronyssinus D. farinae Both | 0% - - - | 100% 28 24 24 | 100% 27 25 25 | - | |
Severity of asthma Grade I Grade II | 10 30 | 29 11 | - | ||
FEV1 (% of predicted) | 91.10 ± 9.666 | 72.62 ± 4.943 | 87.49 ± 2.52 | - | <0.0001 **# |
FEV1/FVC (%) | 91.91 ± 1.849 | 71.14 ± 2.141 | 88.59 ± 8.247 | - | <0.0001 **# |
Total serum IgE (ng/mL) Mean ± SD | 158.3 ± 73.1 | 921.3 ± 317.3 | 798.2 ± 204.0 | - | <0.0001 **# |
FoxO1 gene expression (ng/mL) Mean ± SD | 1.29 ± 0.76 | 1.33 ± 0.43 | 1.82 ± 0.49 | - | <0.0001 **# |
SIRT1 gene expression (ng/mL) Mean ± SD | 1.6 ± 0.52 | 1.2 ± 0.31 | 2.03 ± 0.43 | 41.59 | <0.0001 **$ |
Compared Groups | HV vs. HDM/AA | HV vs. HDM/SCIT | HDM/AA vs. HDM/SCIT |
---|---|---|---|
FEV # | <0.0001 ** | 0.1679 ns | <0.0001 ** |
FEV1/FVC% # | <0.0001 ** | >0.9999 ns | <0.0001 ** |
Total serum IgE (ng/mL) # | <0.0001 ** | <0.0001 ** | >0.9999 ns |
FoxO1 gene expression (ng/mL) # | >0.9999 ns | <0.0001 ** | 0.0004 ** |
Sirtuin 1 gene expression (ng/mL) $ | 0.0002 ** | <0.0001 ** | <0.0001 ** |
HV | HDM/AA | HDM/SCIT | ||||
---|---|---|---|---|---|---|
Characteristics | r | p-Value | r | p-Value | r | p-Value |
FoxO1 (ng/mL) | 0.4338 | 0.0052 ** | −0.3108 | 0.0510 ns | −0.01030 | 0.9497 ns |
SIRT1 (ng/mL) | −0.1120 | 0.4915 ns | −0.3321 | 0.0363 * | 0.08908 | 0.5847 ns |
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Mohamed, R.A.; Fakhr, A.E.; Baioumy, S.A. Investigating Forkhead Box O Transcription Factor 1 Gene’s Relation to Immunoglobulin E in House Dust Mite-Allergic Asthma Patients. Adv. Respir. Med. 2023, 91, 532-545. https://doi.org/10.3390/arm91060039
Mohamed RA, Fakhr AE, Baioumy SA. Investigating Forkhead Box O Transcription Factor 1 Gene’s Relation to Immunoglobulin E in House Dust Mite-Allergic Asthma Patients. Advances in Respiratory Medicine. 2023; 91(6):532-545. https://doi.org/10.3390/arm91060039
Chicago/Turabian StyleMohamed, Rania A., Ahmed ElSadek Fakhr, and Shereen A. Baioumy. 2023. "Investigating Forkhead Box O Transcription Factor 1 Gene’s Relation to Immunoglobulin E in House Dust Mite-Allergic Asthma Patients" Advances in Respiratory Medicine 91, no. 6: 532-545. https://doi.org/10.3390/arm91060039