New Development of Disaster-Related and Tropical Infectious Diseases Control

Round 1

Reviewer 1 Report

New development of disaster-related and tropical infectious diseases control 

 

Dear author and editor: 

This is an interesting review article talked about a serious problem which could increase the risk of infectious disease outbreaks, suggested DNA hybridization method to identify some pathogens related to DRID and plasma level of matricellular protein to determine the severity of infection. 

This article could be published in reports after a minor revision: 

• How do explain the increasing rates of HIV/MDR-TB cases? could be because of emerging of new Mtb strains from different geographic positions?
• Do you think that the spread of DRID could be related to the new strains of the pathogens?
• You could add references talking more about the MCPs proteins during Mtb infection?
• Is there any clinical data about the effectiveness of the OPN inhibition in DRID control?

Thank you very much, best regards 

Author Response

Q1  How do explain the increasing rates of HIV/MDR-TB cases? could be because of emerging of new Mtb strains from different geographic positions?

A1. Thank you for your question.  We do not think it is specific to HIV/MDR-TB but also  HIV rates among TB is increasing (recently 40-50% of MTB patients are HIV(+)) (unpublished).  We therefore described the reasons of high rates of MDR-TB in the Philippine (Lines 142-145).

Q2. Do you think that the spread of DRID could be related to the new strains of the pathogens?

A2.  Thank you for an interesting question. The genotype of MTB  exsmined in Manila at two different sites were described. We can not conclude the new strains are responsible for MDR-TB. (Lines 145-148)

Q3. You could add references talking more about the MCPs proteins during Mtb infection?

A3. Thank you very much for your advise. According to the suggestions, we described the roles of OPN in granuloma formation in MTB infection. (Lines 292-310)

Q4.Is there any clinical data about the effectiveness of the OPN inhibition in DRID control?

A4. Thank you for your interest.  We have not started any clinical trial by brefelamide derivatives.

Author Response File: Author Response.docx

Reviewer 2 Report

In this manuscript, authors present recent advances for the control of infectious disease and more specifically for disaster-related infectious diseases (DRID). They detailed a dipstick DNA chromatography assay termed as Single-Tag Hybridization-Printed Array Strip (STH-PAS) to detect low concentration of dengue virus serotypes. Moreover, authors discussed potential biomarkers for acute dengue infection such as Gal-9.

The manuscript is of interest to the readers and suitable for publication in Reports after minor modifications.

The following suggestions need to be considered:

- Manuscript need major editing improvement (syntax are sometime missed, bacterial species name should be in italics, homogenize [x]. or .[x] for references).

- Could be interesting to introduce more deeply tuberculosis and HIV in the 2.1. paragraph.

- Figure 2 need to be improved (increase quality, use colors, replace “tenperature” in panel b.

-Figure 4: replace “tenmplate”

-line 272: do you mean epitope?

-immunology items should be detailed in the manuscript to be more accessible (TH1 cells, CD8 cells, CD44, etc).

Author Response

Q1Manuscript need major editing improvement (syntax are sometime missed, bacterial species name should be in italics, homogenize [x]. or .[x] for references).

A1. Thank you for your advice we have tried to improve the manuscript all the corrected parts are expressed in red in the attached file.

Q2.Could be interesting to introduce more deeply tuberculosis and HIV in the 2.1. paragraph.

A2. Thank you for your encouragement. We described the importance from public health view (Line 106-108) and immunological view of HIV/TB (1190126) in the attached revised file.

Q3. Figure 2 need to be improved (increase quality, use colors, replace “temperature” in panel b.

A3:  The quality of Fig. 2  was improved.

Q4. Figure 4: replace “tenmplate”

A4: It was written as template. 

Q 5: line 272: do you mean epitope?

A5: It was corrected at line 285. 

Q6.immunology items should be detailed in the manuscript to be more accessible (TH1 cells, CD8 cells, CD44, etc).

A6. Thank you for your comments. Each word was explained. Line 243, 247-248.

Author Response File: Author Response.docx