Polysaccharide as a Separation Medium for Gel Electrophoresis
Abstract
:1. Introduction
2. Mechanism of Molecular Sieving
3. Polysaccharide vs. Polyacrylamide
4. Agarose Gel vs. Protein Gel
5. Denaturing vs. Native Gel Electrophoresis
6. Effect of Small Pore Structure
7. Application of Agarose Gel Electrophoresis
7.1. Nucleic Acids
7.1.1. Gel Shift Assay
7.1.2. Comet Assay
7.2. Proteins
7.2.1. Native Gel Electrophoresis
7.2.2. Activity Staining
7.2.3. Detection of Contamination
7.2.4. Purification of Protein by Gel Extraction
7.2.5. Native Western Blotting by Contact
8. Conclusions
Funding
Acknowledgments
Conflicts of Interest
References
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Arakawa, T.; Nakagawa, M.; Sakuma, C.; Tomioka, Y.; Kurosawa, Y.; Akuta, T. Polysaccharide as a Separation Medium for Gel Electrophoresis. Polysaccharides 2024, 5, 380-398. https://doi.org/10.3390/polysaccharides5030024
Arakawa T, Nakagawa M, Sakuma C, Tomioka Y, Kurosawa Y, Akuta T. Polysaccharide as a Separation Medium for Gel Electrophoresis. Polysaccharides. 2024; 5(3):380-398. https://doi.org/10.3390/polysaccharides5030024
Chicago/Turabian StyleArakawa, Tsutomu, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, and Teruo Akuta. 2024. "Polysaccharide as a Separation Medium for Gel Electrophoresis" Polysaccharides 5, no. 3: 380-398. https://doi.org/10.3390/polysaccharides5030024