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Article

An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green

by
Danaya V. Demidenko
1,
Nataliya V. Varlamova
1,
Taisiya M. Soboleva
2,
Aleksandra V. Shitikova
2 and
Marat R. Khaliluev
1,2,*
1
All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127434 Moscow, Russia
2
Agrobiotechnology Institute, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya 49, 127434 Moscow, Russia
*
Author to whom correspondence should be addressed.
BioTech 2024, 13(3), 25; https://doi.org/10.3390/biotech13030025
Submission received: 12 April 2024 / Revised: 24 June 2024 / Accepted: 3 July 2024 / Published: 4 July 2024
(This article belongs to the Section Agricultural and Food Biotechnology)

Abstract

Aseptic seedlings of different ages derived from surface-sterilized mature seeds were applied as an explant source. Various explants such as 7- and 21-day-old hypocotyl fragments, 42-day-old nodal stem segments, and transverse nodal segments of stem, as well as leaf petioles, were cultured on the agar-solidified Murashige and Skoog (MS) basal medium supplemented with 0.1 mg/L IAA, 5 mg/L AgNO3 and different types and concentrations of cytokinin (1 mg/L zeatin, 0.25 mg/L thidiazuron (TDZ), and 5 mg/L 6-benzylaminopurine (6-BAP)). Consequently, it was found that 7- and 21-day-old hypocotyl fragments, as well as nodal stem segments obtained from adult aseptic seedlings, are characterized by a high explant viability and callus formation capacity with a frequency of 79.7–100%. However, the success of in vitro somatic shoot organogenesis was significantly determined not only by the culture medium composition and explant type but also depending on its age, as well as on the size and explant preparation in cases of hypocotyl and age-matched nodal stem fragments, respectively. Multiple somatic shoot organogenesis (5.7 regenerants per explant) with a frequency of 67.5% was achieved during 3 subcultures of juvenile hypocotyl-derived callus tissue on MS culture medium containing 0.25 mg/L TDZ as cytokinin source. Castor bean regenerants were excised from the callus and successfully rooted on ½ MS basal medium without exogenous auxin (81%). In vitro plantlets with well-developed roots were adapted to ex vitro conditions with a frequency of 90%.
Keywords: efficiency of indirect in vitro shoot organogenesis; explant source; morphological characteristics of callus tissue; plant growth regulators (PGRs); plantlets adaptation to ex vitro conditions; Ricinus communis L.; shoot rhizogenesis efficiency of indirect in vitro shoot organogenesis; explant source; morphological characteristics of callus tissue; plant growth regulators (PGRs); plantlets adaptation to ex vitro conditions; Ricinus communis L.; shoot rhizogenesis

Share and Cite

MDPI and ACS Style

Demidenko, D.V.; Varlamova, N.V.; Soboleva, T.M.; Shitikova, A.V.; Khaliluev, M.R. An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green. BioTech 2024, 13, 25. https://doi.org/10.3390/biotech13030025

AMA Style

Demidenko DV, Varlamova NV, Soboleva TM, Shitikova AV, Khaliluev MR. An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green. BioTech. 2024; 13(3):25. https://doi.org/10.3390/biotech13030025

Chicago/Turabian Style

Demidenko, Danaya V., Nataliya V. Varlamova, Taisiya M. Soboleva, Aleksandra V. Shitikova, and Marat R. Khaliluev. 2024. "An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green" BioTech 13, no. 3: 25. https://doi.org/10.3390/biotech13030025

APA Style

Demidenko, D. V., Varlamova, N. V., Soboleva, T. M., Shitikova, A. V., & Khaliluev, M. R. (2024). An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green. BioTech, 13(3), 25. https://doi.org/10.3390/biotech13030025

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