Next Article in Journal / Special Issue
RNF6 as an Oncogene and Potential Therapeutic Target—A Review
Previous Article in Journal
Metabolomics Community in Russia: History of Development, Key Participants, and Results
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
BioTech
Volume 9
Issue 4
10.3390/biotech9040021
biotech-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Transcriptome Sequencing of the Striped Cucumber Beetle, Acalymma vittatum (F.), Reveals Numerous Sex-Specific Transcripts and Xenobiotic Detoxification Genes

by
Michael E. Sparks
1,
David R. Nelson
2,
Ariela I. Haber
1,
Donald C. Weber
1 and
Robert L. Harrison
1,*
1
Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS, Beltsville, MD 20705, USA
2
Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA
*
Author to whom correspondence should be addressed.
BioTech 2020, 9(4), 21; https://doi.org/10.3390/biotech9040021
Submission received: 24 September 2020 / Revised: 21 October 2020 / Accepted: 22 October 2020 / Published: 27 October 2020
(This article belongs to the Special Issue Feature Papers 2020)
Browse Figures
Versions Notes

Abstract

Acalymma vittatum (F.), the striped cucumber beetle, is an important pest of cucurbit crops in the contintental United States, damaging plants through both direct feeding and vectoring of a bacterial wilt pathogen. Besides providing basic biological knowledge, biosequence data for A. vittatum would be useful towards the development of molecular biopesticides to complement existing population control methods. However, no such datasets currently exist. In this study, three biological replicates apiece of male and female adult insects were sequenced and assembled into a set of 630,139 transcripts (of which 232,899 exhibited hits to one or more sequences in NCBI NR). Quantitative analyses identified 2898 genes differentially expressed across the male–female divide, and qualitative analyses characterized the insect’s resistome, comprising the glutathione S-transferase, carboxylesterase, and cytochrome P450 monooxygenase families of xenobiotic detoxification genes. In summary, these data provide useful insights into genes associated with sex differentiation and this beetle’s innate genetic capacity to develop resistance to synthetic pesticides; furthermore, these genes may serve as useful targets for potential use in molecular-based biocontrol technologies.
Keywords: striped cucumber beetle; sex-specific transcripts; xenobiotic detoxification; resistome; glutathione S-transferase; carboxylesterase; cytochrome P450; transcriptomics striped cucumber beetle; sex-specific transcripts; xenobiotic detoxification; resistome; glutathione S-transferase; carboxylesterase; cytochrome P450; transcriptomics

Share and Cite

MDPI and ACS Style

Sparks, M.E.; Nelson, D.R.; Haber, A.I.; Weber, D.C.; Harrison, R.L. Transcriptome Sequencing of the Striped Cucumber Beetle, Acalymma vittatum (F.), Reveals Numerous Sex-Specific Transcripts and Xenobiotic Detoxification Genes. BioTech 2020, 9, 21. https://doi.org/10.3390/biotech9040021

AMA Style

Sparks ME, Nelson DR, Haber AI, Weber DC, Harrison RL. Transcriptome Sequencing of the Striped Cucumber Beetle, Acalymma vittatum (F.), Reveals Numerous Sex-Specific Transcripts and Xenobiotic Detoxification Genes. BioTech. 2020; 9(4):21. https://doi.org/10.3390/biotech9040021

Chicago/Turabian Style

Sparks, Michael E., David R. Nelson, Ariela I. Haber, Donald C. Weber, and Robert L. Harrison. 2020. "Transcriptome Sequencing of the Striped Cucumber Beetle, Acalymma vittatum (F.), Reveals Numerous Sex-Specific Transcripts and Xenobiotic Detoxification Genes" BioTech 9, no. 4: 21. https://doi.org/10.3390/biotech9040021

APA Style

Sparks, M. E., Nelson, D. R., Haber, A. I., Weber, D. C., & Harrison, R. L. (2020). Transcriptome Sequencing of the Striped Cucumber Beetle, Acalymma vittatum (F.), Reveals Numerous Sex-Specific Transcripts and Xenobiotic Detoxification Genes. BioTech, 9(4), 21. https://doi.org/10.3390/biotech9040021

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop