Exploring the Distribution of Low Molecular Weight Compounds in Water-Based Two-Phase Systems with Various Salt Additives
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.1.1. Polymers
2.1.2. Other Chemicals
2.2. Methods
Partitioning
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ATPS | Polymer 1 | Polymer 2 | Total Composition | |
---|---|---|---|---|
Polymer 1 | Polymer 2 | |||
S1 | Dextran | Ficoll | 12.9 | 18.1 |
S2 | Dextran | PEG4000 | 13.67 | 6.15 |
S3 | Dextran | Ucon | 12.39 | 10.08 |
S4 | Ficoll | PEG8000 | 24.67 | 10.42 |
S5 | Ficoll | Ucon | 19.12 | 15.47 |
S6 | Peg8000 | Ucon | 15.00 | 29.97 |
Distribution Coefficients of Studied Compounds | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NaPB 0.01 M, pH 7.4 | NaCl 0.15 M + NaPB 0.01 M, pH 7.4 | ||||||||||||
ATPS | ATPS | ||||||||||||
Compounds | S1 | S2 | S3 | S4 | S5 | S6 | Compounds | S1 | S2 | S3 | S4 | S5 | S6 |
Phenol | 1.29 ± 0.02 | 1.690 ± 0.006 | 3.16 ± 0.03 | 1.84 ± 0.01 | 3.55 ± 0.05 | 3.35 ± 0.06 | Phenol b | 1.21 ± 0.03 | 1.663 ± 0.003 | 3.23± 0.02 | 1.74 ± 0.01 | 3.82 ± 0.01 | 4.28 ± 0.05 |
Benzyl alcohol | 1.16 ± 0.01 | 1.370 ± 0.006 | 2.09 ±0.01 | 1.553 ± 0.004 | 2.29 ± 0.02 | 2.21 ± 0.01 | Benzyl alcohol | 1.16 ± 0.01 | 1.41 ± 0.01 | 2.245 ± 0.006 | 1.54 ± 0.01 | 2.34 ± 0.02 | 2.54 ± 0.03 |
2-Phenylethanol | 1.17 ± 0.02 | 1.48 ± 0.04 | 1.93 ± 0.08 | 1.51 ± 0.03 | 2.22 ± 0.08 | 2.43 ± 0.04 | 2-Phenylethanol | 1.03 ± 0.03 | 1.48 ± 0.02 | 2.4 ± 0.1 | 1.60 ± 0.01 | 2.50 ± 0.06 | 3.11 ± 0.07 |
Vanillin | 1.308 ± 0.008 | 1.65 ± 0.01 | 3.19 ± 0.02 | 1.632 ± 0.008 | 3.14 ± 0.02 | 2.59 ± 0.02 | Vanillin b | 1.19 ± 0.01 | 1.69 ± 0.01 | 3.37 ± 0.04 | 1.90 ± 0.02 | 3.98 ± 0.05 | 3.85 ± 0.04 |
4-nitrophenyl-α-D-glucopyranoside | 1.127 ± 0.003 | 1.122 ± 0.004 | 1.508 ± 0.006 | 1.099 ± 0.003 | 1.63 ± 0.01 | 2.22 ± 0.01 | 4-nitrophenyl-α-D-glucopyranoside | 1.155 ± 0.002 | 1.106 ± 0.005 | 1.61 ± 0.01 | 1.133 ± 0.004 | 1.87 ± 0.01 | 2.685 ± 0.004 |
Coumarin | 1.244 ± 0.002 | 1.378 ± 0.004 | 2.55 ± 0.01 | 1.363 ± 0.004 | 2.67 ± 0.02 | 2.92 ± 0.02 | Coumarin b | 1.164 ± 0.007 | 1.34 ± 0.01 | 2.51 ± 0.05 | 1.402 ± 0.004 | 2.94 ± 0.05 | 3.92 ± 0.02 |
Methyl anthranilate | 1.266 ± 0.004 | 1.583 ± 0.004 | 3.34 ± 0.02 | 1.57 ± 0.01 | 3.65 ± 0.01 | 4.41 ± 0.03 | Methyl anthranilate b | 1.253 ± 0.006 | 1.623 ± 0.008 | 3.52 ± 0.02 | 1.694 ± 0.007 | 4.81 ± 0.08 | 5.92 ± 0.07 |
Na2SO4 0.1 M + NaPB 0.01 M, pH 7.4 | NaClO4 0.15 M + NaPB 0.01 M, pH 7.4 | ||||||||||||
ATPS | ATPS | ||||||||||||
Compounds | S1 | S2 | S3 | S4 | S5 | S6 a | Compounds | S1 | S2 | S3 | S4 | S5 | S6 |
Phenol | 1.41 ± 0.01 | 2.004 ± 0.006 | 5.14 ± 0.05 | 2.316 ± 0.006 | 6.56 ± 0.04 | - | Phenol | 1.282 ± 0.002 | 1.81 ± 0.01 | 3.84 ± 0.02 | 2.14 ± 0.01 | 4.21 ± 0.02 | 3.04 ± 0.02 |
Benzyl alcohol | 1.18 ± 0.02 | 1.649 ± 0.008 | 2.85 ± 0.03 | 1.79 ± 0.02 | 3.63 ± 0.02 | - | Benzyl alcohol | 1.121 ± 0.004 | 1.48 ± 0.02 | 2.31 ± 0.01 | 1.664 ± 0.005 | 2.49 ± 0.01 | 2.15 ± 0.01 |
2-Phenylethanol | 1.36 ± 0.02 | 1.46 ± 0.07 | 2.32 ± 0.06 | 1.54 ± 0.04 | 3.72 ± 0.08 | - | 2-Phenylethanol | 1.13 ± 0.04 | 1.46 ± 0.02 | 2.66 ± 0.07 | 1.66 ± 0.04 | 2.65 ± 0.07 | 2.46 ± 0.05 |
Vanillin | 1.475 ± 0.004 | 1.94 ± 0.01 | 5.11 ± 0.03 | 2.168 ± 0.005 | 5.23 ± 0.05 | - | Vanillin | 1.223 ± 0.008 | 1.758 ± 0.008 | 3.62 ± 0.02 | 1.862 ± 0.006 | 3.72 ± 0.04 | 2.559 ± 0.006 |
4-nitrophenyl-α-D-glucopyranoside | 1.21 ± 0.02 | 1.242 ± 0.008 | 2.03 ± 0.01 | 1.25 ± 0.01 | 2.37 ± 0.02 | - | 4-nitrophenyl-α-D-glucopyranoside | 1.079 ± 0.005 | 1.214 ± 0.006 | 1.585 ± 0.006 | 1.187 ± 0.003 | 1.771 ± 0.007 | 2.02 ± 0.01 |
Coumarin | 1.358 ± 0.005 | 1.684 ± 0.006 | 3.60 ± 0.02 | 1.686 ± 0.008 | 4.679 ± 0.009 | - | Coumarin | 1.219 ± 0.005 | 1.54 ± 0.01 | 3.09 ± 0.02 | 1.573 ± 0.004 | 3.24 ± 0.02 | 2.977 ± 0.009 |
Methyl anthranilate | 1.463 ± 0.005 | 2.015 ± 0.003 | 6.54 ± 0.02 | 2.10 ± 0.02 | 7.47 ± 0.04 | - | Methyl anthranilate | 1.294 ± 0.008 | 1.828 ± 0.004 | 4.69 ± 0.02 | 1.935 ± 0.004 | 5.02 ± 0.01 | 4.25 ± 0.04 |
Solute | ai | bi | ai | bi | ai | bi |
---|---|---|---|---|---|---|
NaCl | Na2SO4 | NaClO4 | ||||
Phenol | −0.06±0.04 | 1.20±0.09 | −0.03±0.02 | 1.51±0.04 | 0.02±0.05 | 1.1±0.1 |
Benzyl alcohol | −0.01±0.02 | 1.12±0.08 | −0.02±0.03 | 1.6±0.1 | 0.00±0.03 | 1.1±0.1 |
2-Phenylethanol | −0.07±0.03 | 1.5±0.1 | −0.05±0.08 | 1.6±0.4 | −0.01±0.06 | 1.2±0.2 |
Vanillin | −0.03±0.07 | 1.3±0.2 | 0.01±0.02 | 1.42±0.07 | −0.01±0.04 | 1.1±0.1 |
4-nitrophenyl-α-D-glucopyranoside | −0.01±0.01 | 1.26±0.04 | 0.01±0.01 | 1.69±0.09 | 0.03±0.02 | 0.9±0.1 |
Coumarin | −0.04±0.04 | 1.2±0.1 | 0.02±0.03 | 1.4±0.1 | 0.03±0.03 | 1.1±0.1 |
Methyl anthranilate | −0.02±0.03 | 1.21±0.07 | 0.01±0.01 | 1.54±0.02 | 0.05±0.06 | 1.1±0.1 |
Solute | di,0 | PSA | SASA | MP | MR |
---|---|---|---|---|---|
Benzyl alcohol | 0.089 | 20.23 | 177.93 | 12.79 | 32.874 |
2-Phenylethanol | 0.273 | 20.23 | 208.55 | 14.56 | 37.629 |
Vanillin | 0.102 | 46.53 | 220.74 | 15.36 | 41.086 |
4-nitrophenyl-α-D-glucopyranoside | 0.223 | 142.52 | 390.32 | 26.65 | 66.504 |
Coumarin | 0.073 | 26.3 | 186.18 | 15.7 | 41.549 |
Methyl anthranilate | 0.022 | 52.32 | 222.14 | 15.89 | 42.784 |
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Costa, M.; Madeira, P.P.; Uversky, V.N.; Zaslavsky, B.Y. Exploring the Distribution of Low Molecular Weight Compounds in Water-Based Two-Phase Systems with Various Salt Additives. Physchem 2024, 4, 334-343. https://doi.org/10.3390/physchem4030023
Costa M, Madeira PP, Uversky VN, Zaslavsky BY. Exploring the Distribution of Low Molecular Weight Compounds in Water-Based Two-Phase Systems with Various Salt Additives. Physchem. 2024; 4(3):334-343. https://doi.org/10.3390/physchem4030023
Chicago/Turabian StyleCosta, Margarida, Pedro P. Madeira, Vladimir N. Uversky, and Boris Y. Zaslavsky. 2024. "Exploring the Distribution of Low Molecular Weight Compounds in Water-Based Two-Phase Systems with Various Salt Additives" Physchem 4, no. 3: 334-343. https://doi.org/10.3390/physchem4030023