SynBio, Volume 3, Issue 4

Nucleic acid-based gene interfering and editing molecules, such as antisense oligonucleotides, ribozymes, small interfering RNAs (siRNAs), and CRISPR-Cas9-associated guide RNAs, are promising gene-targeting agents for therapeutic applications. Cancer...

The CRISPR/SpCas9 system has revolutionized biology by enabling precise and programmable genome modification. While substantial effort has focused on engineering the SpCas9 protein and spacer sequences, the single-guide RNA (sgRNA) scaffold is an equ...

Protein function emerges from dynamic conformational changes, yet structure prediction methods provide only static snapshots. While AlphaFold3 (AF3) predicts protein structures, the potential for extracting dynamic information from its ensemble predi...

Synthetic biology, an emergent interdisciplinary field integrating principles from biology, engineering, and computer science, endeavors to rationally design and construct novel biological systems or reprogram extant ones to achieve predefined functi...

Researchers strive to exploit kinetic potentials of multistep reactions by positioning enzymes in a regulated fashion. Therein, the proliferating cell nuclear antigen (PCNA) from Sulfolobus solfataricus is a promising biomolecular tool due to its ext...

While synthetic biology has traditionally focused on creating biological systems often through genetic engineering, emerging technologies, for example, implantable pacemakers with integrated piezo-electric and tribo-electric materials are beginning t...

Polyproline residues are well known to induce ribosomal stalling during translation. Our previous work demonstrated that inserting a short translation-enhancing peptide, Ser-Lys-Ile-Lys (SKIK), immediately upstream of such difficult-to-translate sequ...