Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Identifier | Passed Reads | Trimmed Reads | Trimmed Read Length | Mapped Reads | Coverage |
---|---|---|---|---|---|
MRG2101 | 444,000 | 435,340 | 200–36,686 | 1411 | 23–89 |
MRG2102 | 1,272,000 | 515,045 | 200–30,400 | 1220 | 29–93 |
MRG2103 | 372,000 | 362,438 | 200–40,124 | 1327 | 38–83 |
MRG2104 | 236,000 | 235,969 | 50–25,996 | 709 | 14–64 |
MRG2105 | 972,000 | 892,446 | 200–48,837 | 7838 | 39–145 |
MRG2106 | 844,000 | 841,533 | 105–34,665 | 2693 | 81–170 |
MRG2107 | 1,760,000 | 892,394 | 500–82,653 | 2563 | 84–227 |
Gene | Full Gene Name | Length (bp) | Variable Sites (S) | Percent Variable | Haplotypes (h) | Haplotype Diversity (Hd) | Nucleotide Diversity (π) |
---|---|---|---|---|---|---|---|
12S | 12S ribosomal RNA | 721 | 8 | 1.11% | 4 | 0.714 | 0.00317 |
16S | 16S ribosomal RNA | 1197 | 23 | 1.92% | 6 | 0.952 | 0.00597 |
ATP6 | ATP synthase membrane subunit 6 | 666 | 22 | 3.30% | 7 | 1.000 | 0.01044 |
ATP8 | ATP synthase membrane subunit 8 | 156 | 4 | 2.56% | 4 | 0.714 | 0.00733 |
CYTB | Cytochrome b | 1119 | 42 | 3.75% | 6 | 0.952 | 0.01170 |
COI | Cytochrome c oxidase I | 1539 | 39 | 2.53% | 7 | 1.000 | 0.00792 |
COII | Cytochrome c oxidase II | 670 | 15 | 2.24% | 7 | 1.000 | 0.00739 |
COIII | Cytochrome c oxidase III | 781 | 20 | 2.56% | 4 | 0.714 | 0.00756 |
NAD1 | NADH dehydrogenase subunit 1 | 924 | 26 | 2.81% | 7 | 1.000 | 0.00948 |
NAD2 | NADH dehydrogenase subunit 2 | 962 * | 25 | 2.60% | 7 | 1.000 | 0.00832 |
NAD3 | NADH dehydrogenase subunit 3 | 348 | 14 | 4.02% | 5 | 0.905 | 0.01286 |
NAD4 | NADH dehydrogenase subunit 4 | 1275 | 39 | 3.06% | 6 | 0.952 | 0.01083 |
NAD4L | NADH dehydrogenase subunit 4L | 288 | 10 | 3.47% | 4 | 0.810 | 0.01157 |
NAD5 | NADH dehydrogenase subunit 5 | 1698 | 53 | 3.12% | 7 | 1.000 | 0.00937 |
NAD6 | NADH dehydrogenase subunit 6 | 453 | 14 | 3.09% | 6 | 0.952 | 0.01092 |
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Graham, M.R.; Santibáñez-López, C.E.; Zehnpfennig, J.R.; Tillman, D.S.; Murdoch, B. Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing. Arthropoda 2024, 2, 119-129. https://doi.org/10.3390/arthropoda2020009
Graham MR, Santibáñez-López CE, Zehnpfennig JR, Tillman DS, Murdoch B. Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing. Arthropoda. 2024; 2(2):119-129. https://doi.org/10.3390/arthropoda2020009
Chicago/Turabian StyleGraham, Matthew R., Carlos E. Santibáñez-López, Jessica R. Zehnpfennig, Dylan S. Tillman, and Barbara Murdoch. 2024. "Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing" Arthropoda 2, no. 2: 119-129. https://doi.org/10.3390/arthropoda2020009