N-Acetylaspartylglutamate (NAAG) Pretreatment Reduces Hypoxic-Ischemic Brain Damage and Oxidative Stress in Neonatal Rats
Abstract
:1. Introduction
2. Material and Methods
2.1. Ethics Approval and Consent for Participation
2.2. Experimental Hypoxia-Ischemia
2.3. Drug Application
2.4. Evaluation of Brain Damage
2.5. Tissue Preparation for Biochemical Analysis
2.6. Determination of ROS Level
2.7. Determination of Glutathione Concentration
2.8. Determination of Antioxidant Enzyme Activity
2.8.1. Superoxide Dismutase
2.8.2. Glutathione Peroxidase (GPx)
2.8.3. Catalase
2.9. Determination of TGF-β Concentration
2.10. Statistical Analysis
3. Results
3.1. The Effect of NAAG Application on HI-Induced Brain Damage
3.2. The Effect of NAAG Application on Changes in ROS Level in Rat Brain after HI
3.3. The Effect of NAAG Application on HI Induced Changes in SOD Activity
3.4. The Effect of NAAG Application on Changes in GPx Activity after HI
3.5. The Effect of the NAAG Application on Changes in GSH Level after HI
3.6. The Effect of NAAG Application on the Changes in Catalase Activity Observed after HI
3.7. The Effect of NAAG Application on Changes in the TGF-β Concentration after HI
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Bratek, E.; Ziembowicz, A.; Salinska, E. N-Acetylaspartylglutamate (NAAG) Pretreatment Reduces Hypoxic-Ischemic Brain Damage and Oxidative Stress in Neonatal Rats. Antioxidants 2020, 9, 877. https://doi.org/10.3390/antiox9090877
Bratek E, Ziembowicz A, Salinska E. N-Acetylaspartylglutamate (NAAG) Pretreatment Reduces Hypoxic-Ischemic Brain Damage and Oxidative Stress in Neonatal Rats. Antioxidants. 2020; 9(9):877. https://doi.org/10.3390/antiox9090877
Chicago/Turabian StyleBratek, Ewelina, Apolonia Ziembowicz, and Elzbieta Salinska. 2020. "N-Acetylaspartylglutamate (NAAG) Pretreatment Reduces Hypoxic-Ischemic Brain Damage and Oxidative Stress in Neonatal Rats" Antioxidants 9, no. 9: 877. https://doi.org/10.3390/antiox9090877