Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model
Abstract
:1. Introduction
2. Results
2.1. Severe Bone Resorption after Ligation in Aged Mice
2.2. Increased Inflammatory Cell Accumulation at the Periodontal Bone Destruction Area in Aged Mice
2.3. Decreased MSCs Number in Aged Mice
2.4. Functional Impairment of Aged MSCs
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Periodontitis Model
4.3. Micro CT Analysis
4.4. Histological Analysis
4.5. Immunohistochemical Analysis
4.6. Cell Culture
4.7. Colony-Forming Unit Fibroblastic (CFU-f) Assay
4.8. In Vitro Wound-Healing Assay
4.9. Cell Surface Antigen Analysis
4.10. In Vitro Osteogenic and Adipogenic Differentiation
4.11. Reverse Transcription and Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
4.12. Senescence Detection Assay
4.13. Isolation, Activation, and Culture of T Cells
4.14. Induction of T Cell Apoptosis
4.15. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | GenBank Registration Number | Primer Set | PCR Product Length (bp) | |
---|---|---|---|---|
s29 | NM_009093 | Sense: | 5’-GGAGTCACCCACGGAAGTTCGG-3’ | 108 |
Anti-sense: | 5’-GGAAGCACTGGCGGCACATG-3’ | |||
Alp | JO2980 | Sense: | 5’-GCTCTCCCTACCGACCCTGTTC-3’ | 130 |
Anti-sense: | 5’-TGCTGGAAGTTGCCTGGACCTC-3’ | |||
Ocn | BC055868 | Sense: | 5’-CCAAGCAGGAGGGCAATAAGGTAG-3’ | 122 |
Anti-sense: | 5’-CTCGTCACAAGCAGGGTCAAGC-3’ | |||
Lpl | BC003305 | Sense: | 5’-GTCTGGCTGACACTGGACAA-3’ | 221 |
Anti-sense: | 5’-TGGGCCATTAGATTCCTCAC-3’ | |||
Pparγ | NM_011146 | Sense: | 5’-GCCAAGGTGCTCCAGAAGATGA-3’ | 155 |
Anti-sense: | 5’-CGGGTGGGACTTTCCTGCTAAT-3’ | |||
Tnf-α | BC117057 | Sense: | 5’-GTGGAACTGGCAGAAGAG-3’ | 96 |
Anti-sense: | 5’-CACAAGCAGGAATGAGAAGA-3’ | |||
Il1-β | M15131 | Sense: | 5’-AATCTCACAGCAGCACATC-3’ | 194 |
Anti-sense: | 5’-CCAGCAGGTTATCATCATCAT-3’ | |||
Ifn-γ | BC119065 | Sense: | 5’-AGCTCATCCGAGTGGTCC-3’ | 88 |
Anti-sense: | 5’-CTCTTCCCCACCCCGAAT-3’ |
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Aung, K.T.; Akiyama, K.; Kunitomo, M.; Mun, A.Y.; Tosa, I.; Nguyen, H.T.T.; Zhang, J.; Kohno, T.; Ono, M.; Hara, E.S.; et al. Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model. Int. J. Mol. Sci. 2020, 21, 8103. https://doi.org/10.3390/ijms21218103
Aung KT, Akiyama K, Kunitomo M, Mun AY, Tosa I, Nguyen HTT, Zhang J, Kohno T, Ono M, Hara ES, et al. Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model. International Journal of Molecular Sciences. 2020; 21(21):8103. https://doi.org/10.3390/ijms21218103
Chicago/Turabian StyleAung, Kyaw Thu, Kentaro Akiyama, Masayoshi Kunitomo, Aung Ye Mun, Ikue Tosa, Ha Thi Thu Nguyen, Jiewen Zhang, Teisaku Kohno, Mitsuaki Ono, Emilio Satoshi Hara, and et al. 2020. "Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model" International Journal of Molecular Sciences 21, no. 21: 8103. https://doi.org/10.3390/ijms21218103