ROS-Dependent ER Stress and Autophagy Mediate the Anti-Tumor Effects of Tributyltin (IV) Ferulate in Colon Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. The Cytotoxic Effects of TBT-F Are Dependent on ROS Production
2.2. The Role of the Protective Factor Nrf2 and the Effect of Nrf2 Silencing
2.3. TBT-F Induces ER Stress Markers and NAC Prevents These Effects
2.4. TBT-F Activates ROS-Dependent Autophagy Correlated with ER Stress
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Cell Culture Conditions
4.3. Evaluation of Cell Viability
4.4. Lactate Dehydrogenase Release Assay
4.5. Clonogenic Assay
4.6. Intracellular ROS Measurement
4.7. Monodansylcadaverine Labeling
4.8. Western Blot Analysis
4.9. Gene Silencing
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Celesia, A.; Morana, O.; Fiore, T.; Pellerito, C.; D’Anneo, A.; Lauricella, M.; Carlisi, D.; De Blasio, A.; Calvaruso, G.; Giuliano, M.; et al. ROS-Dependent ER Stress and Autophagy Mediate the Anti-Tumor Effects of Tributyltin (IV) Ferulate in Colon Cancer Cells. Int. J. Mol. Sci. 2020, 21, 8135. https://doi.org/10.3390/ijms21218135
Celesia A, Morana O, Fiore T, Pellerito C, D’Anneo A, Lauricella M, Carlisi D, De Blasio A, Calvaruso G, Giuliano M, et al. ROS-Dependent ER Stress and Autophagy Mediate the Anti-Tumor Effects of Tributyltin (IV) Ferulate in Colon Cancer Cells. International Journal of Molecular Sciences. 2020; 21(21):8135. https://doi.org/10.3390/ijms21218135
Chicago/Turabian StyleCelesia, Adriana, Ornella Morana, Tiziana Fiore, Claudia Pellerito, Antonella D’Anneo, Marianna Lauricella, Daniela Carlisi, Anna De Blasio, Giuseppe Calvaruso, Michela Giuliano, and et al. 2020. "ROS-Dependent ER Stress and Autophagy Mediate the Anti-Tumor Effects of Tributyltin (IV) Ferulate in Colon Cancer Cells" International Journal of Molecular Sciences 21, no. 21: 8135. https://doi.org/10.3390/ijms21218135