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DNA-Based Biosensors

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (15 March 2023) | Viewed by 2286

Special Issue Editor

Department of Chemistry, University of South Dakota, 414 East Clark St, Vermillion, SD 57069, USA
Interests: fluorescence biosensing and bioimaging; nanotheranostics; biomedicine

Special Issue Information

Dear Colleagues,

I am pleased to invite you to contribute to our Special Issue of the journal Sensors on “DNA-based Biosensors”. Biosensors have undergone booming growth in recent decades and play important role on disease diagnosis and improvement of human health. With the easy and robust synthesis of DNA sequences, DNA-based biosensors have been widely used in sensing applications for different targets, including proteins, nucleic acids, ions, nanoparticles, microbes, as well as cells. DNA sequence can be used as a recognition unit, such as aptamers, and can also be used to amplify signals with certain amplification techniques, such as rolling circle amplification and strands replacement amplification. Due to these features of DNA sequences, the biosensors based on DNA have been utilized in disease diagnosis, drug discovery, environmental monitoring, and therapeutics with different functions.

For this Special Issue, I invite you to share the new state of the art developments in the field of DNA-based biosensors. The original research or review articles on this topic will be all welcome.

Dr. Xu Wu
Guest Editor

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Published Papers (1 paper)

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Research

13 pages, 2399 KiB  
Article
Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor
by Jana Blaškovičová, Vlastimil Vyskočil, Michal Augustín and Andrea Purdešová
Sensors 2023, 23(7), 3425; https://doi.org/10.3390/s23073425 - 24 Mar 2023
Cited by 3 | Viewed by 1915
Abstract
Engineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological environment of [...] Read more.
Engineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological environment of the body containing NaCl, this has raised concerns about the potentially harmful effects of ingested nanomaterials on human health. Although gold nanoparticles (AuNPs) exhibit great potential for various biomedical applications, there is some inconsistency in the case of the unambiguous genotoxicity of AuNPs due to differences in their shape, size, solubility, and exposure time. A DNA/GCE (DNA/glassy carbon electrode) biosensor was used to study ethanol (EtOH) and NaCl-induced gold nanoparticle aggregation genotoxicity under UV light in this study. The genotoxic effect of dispersed and aggregated negatively charged gold nanoparticles AuNP1 (8 nm) and AuNP2 (30 nm) toward salmon sperm double-stranded dsDNA was monitored by cyclic and square-wave voltammetry (CV, SWV). Electrochemical impedance spectroscopy (EIS) was used for a surface study of the biosensor. The aggregation of AuNPs was monitored by UV-vis spectroscopy. AuNP1 aggregates formed by 30% v/v EtOH and 0.15 mol·L−1 NaCl caused the greatest damage to the biosensor DNA layer. Full article
(This article belongs to the Special Issue DNA-Based Biosensors)
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