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Nanobiosensors for the Detection, Diagnostic and Monitoring of Bacterial or Viral Agents

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Nanosensors".

Deadline for manuscript submissions: closed (15 October 2022) | Viewed by 4691

Special Issue Editor


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Guest Editor
Department of Biological Sciences, Clemson University, Clemson, SC 29634-0314, USA
Interests: microbiology; biofilm; synthetic biology; pathogen-host interaction; nanotechnology; biosensor; drug-resistance

Special Issue Information

Dear Colleagues,

To prevent the spread of infectious diseases, facilitate their treatment, and minimize the economic impact and societal costs associated with infections, the early detection of disease agents is essential. Conventional culturing methods are effective for the detection of bacterial and viral agents. However, they often require the use of multiple enrichment, differential, and/or selective processes that could be time-consuming. In addition, their detection limits could be high and, often, they only serve as qualitative indicators. Many high-throughput detection methods have been developed to reduce the time required for microbe identification and to improve accuracy. Nanotechnologies offer opportunities to further reduce detection time and detection limit, owing to their unique surface properties, greater surface-to-volume ratios, etc.

For this Special Issue entitled “Nanobiosensors for the Detection, Diagnosis, and/or Monitoring of Bacterial or Viral Agents”, we encourage the submission of original research or review manuscripts discussing the utilization of nanotechnologies for the detection, diagnosis, and/or tracking and imaging of bacterial or viral agents in environmental or biological samples. We welcome discussions on the biocompatibility of sensor materials, specificity determination, detection limit, response time, ability to differentiate infectious from non-infectious agents, etc. In addition, we also welcome reports on data acquisition, transmission, sensor energy sources, etc. Finally, thoughts on nanobiosensors’ fate in the environment and in patients and their risk assessment are appreciated. Papers concerning the development of industrial standards and guidelines in relation to working with nanobiosensors are also of interest.

Dr. Tzuen-Rong Tzeng
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Nanotechnology 
  • Biosensor 
  • Surface Functionlization 
  • Detection Limit 
  • Specificity 
  • Rapid Detection 
  • Microfluidic

Published Papers (2 papers)

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Research

14 pages, 6817 KiB  
Communication
Detection of Salmonella Typhimurium with Gold Nanoparticles Using Quartz Crystal Microbalance Biosensor
by Hyun Jung Min, Hansel A. Mina, Amanda J. Deering, J. Paul Robinson and Euiwon Bae
Sensors 2022, 22(22), 8928; https://doi.org/10.3390/s22228928 - 18 Nov 2022
Cited by 5 | Viewed by 1938
Abstract
Demonstration of the Salmonella Typhimurium detection system was shown utilizing a quartz crystal microbalance (QCM) biosensor and signal enhancement by gold nanoparticles. In this study, a benchtop system of a QCM biosensor was utilized for the detection of Salmonella Typhimurium. It was designed [...] Read more.
Demonstration of the Salmonella Typhimurium detection system was shown utilizing a quartz crystal microbalance (QCM) biosensor and signal enhancement by gold nanoparticles. In this study, a benchtop system of a QCM biosensor was utilized for the detection of Salmonella Typhimurium. It was designed with a peristaltic pump system to achieve immobilization of antibodies, detection of Salmonella, and the addition of gold nanoparticles to the sensor. As a series of biochemical solutions were introduced to the surface, the proposed system was able to track the changes in the resonant frequency which were proportional to the variations of mass on the sensor. For antibody immobilization, polyclonal antibodies were immobilized via self-assembled monolayers to detect Salmonella O-antigen. Subsequently, Salmonella Typhimurium was detected by antibodies and the average frequency before and after detecting Salmonella was compared. The highest frequency shifts were −26.91 Hz for 109 CFU/mL while the smallest frequency shift was −3.65 Hz corresponding to 103 CFU/mL. For the specificity tests, non-Salmonella samples such as E. coli, Listeria, and Staphylococcus resulted in low cross-reactivity. For signal amplification, biotinylated antibodies reacted to Salmonella followed by streptavidin—100 nm AuNPs through biotin-avidin interaction. The frequency shifts of 103 CFU/mL showed −28.04 Hz, and consequently improved the limit of detection. Full article
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9 pages, 1759 KiB  
Article
Fast and Sensitive Voltammetric Method for the Determination of Rifampicin on Renewable Amalgam Film Electrode
by Marek Szlósarczyk, Robert Piech, Anna Milc and Urszula Hubicka
Sensors 2021, 21(17), 5792; https://doi.org/10.3390/s21175792 - 28 Aug 2021
Cited by 3 | Viewed by 1949
Abstract
In this work, a new sensitive voltammetric method for the determination of rifampicin without time-consuming preconcentration is presented. The objective was to develop a simple, fast and sensitive voltammetric procedure for the analysis of rifampicin in pharmaceutical products. The cyclic renewable mercury film [...] Read more.
In this work, a new sensitive voltammetric method for the determination of rifampicin without time-consuming preconcentration is presented. The objective was to develop a simple, fast and sensitive voltammetric procedure for the analysis of rifampicin in pharmaceutical products. The cyclic renewable mercury film silver-based electrode (Hg(Ag)FE) was applied as a working electrode for this purpose. The optimal conditions for the determination of rifampicin were defined, in terms of the composition of supporting electrolyte (including pH) and instrumental parameters (potential and time of deposition, step potential, pulse height). The method was validated resulting in a satisfactory linearity range of 0.4–250.0 µgmL−1; the limits of detection and quantification are 0.12 µgmL−1 and 0.4 µgmL−1, respectively; and the repeatability of the method expressed as RSD is 4.1% (n = 6) with a surface area of 10.9 mm2. The proposed method was successfully applied in the analysis of rifampicin in simple and composed pharmaceutical formulations. Full article
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