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Volume 44
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10.3390/cimb44110375
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Article

Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories

by
Dahui Qin
1,*,
Mark Forster
1,
Shital M. Gandhi
1,
Ratilal Akabari
2,
Zhong Zheng
1,
Janella Lal
3 and
Katherine Lovinger
4
1
Molecular Diagnostic Laboratory, Moffitt Cancer Center, Tampa, FL 33612, USA
2
Molecular Diagnostic Laboratory, State University of New York (SUNY), Upstate Medical University, Syracuse, NY 13210, USA
3
Molecular Diagnostic Laboratory, Emory University Hospital, Atlanta, GA 30322, USA
4
Orlando Health (ORMC’s) Molecular Laboratory, Orlando, FL 32806, USA
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2022, 44(11), 5543-5549; https://doi.org/10.3390/cimb44110375
Submission received: 14 September 2022 / Revised: 25 October 2022 / Accepted: 2 November 2022 / Published: 8 November 2022
(This article belongs to the Special Issue Next-Generation Sequencing (NGS) Technique and Personalized Medicine)
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Abstract

Sample identification error is a severe medical error in clinical molecular diagnostic laboratories, which can lead to reporting the wrong results for the patient involved. Sample contamination can also lead to incorrect test reports. Avoiding sample identification error and sample contamination could be life-saving. Sample switch and sample contamination could happen on laboratory bench works, especially when pipetting into multi-well plates. It is difficult to realize such errors during laboratory bench work. Laboratory staff may not be aware of such an error when it happens. DNA fingerprinting technology can be used to determine sample identity and subsequently identify sample switch and sample contamination in the laboratory. Our laboratory has explored the usage of this technology in our quality control process and successfully established that DNA fingerprinting can be used to monitor sample switch and sample contamination in next-generation sequencing and BCR/ABL1 real-time PCR bench work.
Keywords: quality control; sample switch; sample contamination; sample error; DNA fingerprint quality control; sample switch; sample contamination; sample error; DNA fingerprint

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MDPI and ACS Style

Qin, D.; Forster, M.; Gandhi, S.M.; Akabari, R.; Zheng, Z.; Lal, J.; Lovinger, K. Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories. Curr. Issues Mol. Biol. 2022, 44, 5543-5549. https://doi.org/10.3390/cimb44110375

AMA Style

Qin D, Forster M, Gandhi SM, Akabari R, Zheng Z, Lal J, Lovinger K. Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories. Current Issues in Molecular Biology. 2022; 44(11):5543-5549. https://doi.org/10.3390/cimb44110375

Chicago/Turabian Style

Qin, Dahui, Mark Forster, Shital M. Gandhi, Ratilal Akabari, Zhong Zheng, Janella Lal, and Katherine Lovinger. 2022. "Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories" Current Issues in Molecular Biology 44, no. 11: 5543-5549. https://doi.org/10.3390/cimb44110375

APA Style

Qin, D., Forster, M., Gandhi, S. M., Akabari, R., Zheng, Z., Lal, J., & Lovinger, K. (2022). Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories. Current Issues in Molecular Biology, 44(11), 5543-5549. https://doi.org/10.3390/cimb44110375

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