First Description of the Mitogenome and Phylogeny of Culicinae Species from the Amazon Region
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Total DNA Extraction
2.2. Quantification of Total Extracted DNA, Construction of the Genomic Library, and Sequencing
2.3. Pre-Processing of Sequenced Products
2.4. Genomic Assembly
2.5. Analyses of the Obtained Mitochondrial Sequences
2.6. Phylogenetics Analysis
3. Results and Discussion
3.1. Organization, Structure and Content of the Mitogenomes Obtained
3.2. Description of Protein-Coding Genes (PCGs)
3.3. Evolutive Analysis
3.4. Phylogenetic Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Species | ID-Code | Collected | Used | Sex | Collection Site | State | Geographical Coordinates | Year |
---|---|---|---|---|---|---|---|---|
Ad. squamipennis | AR_867100 | 6 | 5 | ♀ | Altamira | Pará | −3.203333, −52.206389 | 2017 |
Ae. fluviatilis | AR_866516 | 13 | 5 | ♀ | Canaã dos Carajás | Pará | −6.496944, −49.878333 | 2019 |
Cq. nigricans | AR_850512 | 45 | 5 | ♀ | Altamira | Pará | −3.203333, −52.206389 | 2017 |
Ps. albipes | AR_859743 | 30 | 5 | ♀ | Belém | Pará | −1.495795, −48.454260 | 2017 |
Ps. ferox | AR_849712 | 14 | 5 | ♀ | Santa Bárbara | Pará | −1.209000, −48.271722 | 2017 |
Species Evaluated | Nucleotide Identity (%)—COI (Barcode DNA) | ||||
---|---|---|---|---|---|
Ad. squamipennis AF417729 | Oc. fluviatilis KF314736 | Cq. nigricans KM593033 | Ps. albipes MN997524 | Ps. ferox MN997516 | |
Ad. squamipennis | 98.99 | - | - | - | - |
Ae. fluviatilis | - | 98.78 | - | - | - |
Cq. nigricans | - | - | 99.85 | - | - |
Ps. albipes | - | - | - | 95.14 | - |
Ps. ferox | - | - | - | - | 99.7 |
Species | Size (bp) | A% | T% | C% | G% | AT% | AT-Skew | GC-Skew |
---|---|---|---|---|---|---|---|---|
Whole mitogenome | ||||||||
Aedeomyia squamipennis | 15,087 | 40.3 | 37.9 | 13.0 | 8.8 | 78.2 | 0.0307 | −0.1927 |
Aedes fluviatilis | 14,910 | 40.4 | 37.1 | 13.3 | 9.2 | 77.5 | 0.0426 | −0.1822 |
Coquillettidia nigricans | 14,789 | 40.0 | 38.9 | 13.3 | 7.8 | 78.9 | 0.0139 | −0.2607 |
Psorophora albipes | 15,137 | 39.3 | 38.6 | 13.1 | 9.0 | 77.9 | 0.0090 | −0.1855 |
Psorophora ferox | 15,307 | 39.1 | 38.5 | 13.2 | 9.1 | 77.6 | 0.0068 | −0.1839 |
PCGs | ||||||||
Aedeomyia squamipennis | 11,170 | 32.6 | 44.1 | 11.3 | 12.0 | 76.7 | −0.1499 | 0.0300 |
Aedes fluviatilis | 11,216 | 32.6 | 43.3 | 11.8 | 12.2 | 75.9 | −0.1410 | 0.0167 |
Coquillettidia nigricans | 11,357 | 33.4 | 44.4 | 10.8 | 11.3 | 77.8 | −0.1414 | 0.0226 |
Psorophora albipes | 11,269 | 32.5 | 44.1 | 11.4 | 12.0 | 76.6 | −0.1514 | 0.0256 |
Psorophora ferox | 11,270 | 32.1 | 44.0 | 11.6 | 12.4 | 76.1 | −0.1564 | 0.0333 |
tRNAs | ||||||||
Aedeomyia squamipennis | 1470 | 40.8 | 38.9 | 8.9 | 11.4 | 79.7 | 0.0238 | 0.1232 |
Aedes fluviatilis | 1486 | 40.4 | 39.4 | 8.7 | 11.4 | 79.8 | 0.0125 | 0.1343 |
Coquillettidia nigricans | 1479 | 41.3 | 39.7 | 8.0 | 11.0 | 81.0 | 0.0198 | 0.1579 |
Psorophora albipes | 1493 | 39.5 | 39.2 | 9.2 | 12.0 | 78.7 | 0.0038 | 0.1321 |
Psorophora ferox | 1494 | 40.1 | 39.3 | 8.8 | 11.8 | 79.4 | 0.0101 | 0.1456 |
rRNAs | ||||||||
Aedeomyia squamipennis | 2160 | 38.9 | 43.8 | 6.0 | 11.3 | 82.7 | −0.0593 | 0.3064 |
Aedes fluviatilis | 2151 | 39.1 | 43.6 | 6.1 | 11.2 | 82.7 | −0.0544 | 0.2948 |
Coquillettidia nigricans | 2097 | 39.6 | 42.7 | 5.4 | 12.2 | 82.3 | −0.0377 | 0.3864 |
Psorophora albipes | 2154 | 39.3 | 42.7 | 6.3 | 11.7 | 82.0 | −0.0415 | 0.3000 |
Psorophora ferox | 2160 | 39.4 | 42.6 | 6.3 | 11.7 | 82.0 | −0.0390 | 0.3000 |
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do Nascimento, B.L.S.; da Silva, F.S.; Nunes-Neto, J.P.; de Almeida Medeiros, D.B.; Cruz, A.C.R.; da Silva, S.P.; da Silva e Silva, L.H.; de Oliveira Monteiro, H.A.; Dias, D.D.; Vieira, D.B.R.; et al. First Description of the Mitogenome and Phylogeny of Culicinae Species from the Amazon Region. Genes 2021, 12, 1983. https://doi.org/10.3390/genes12121983
do Nascimento BLS, da Silva FS, Nunes-Neto JP, de Almeida Medeiros DB, Cruz ACR, da Silva SP, da Silva e Silva LH, de Oliveira Monteiro HA, Dias DD, Vieira DBR, et al. First Description of the Mitogenome and Phylogeny of Culicinae Species from the Amazon Region. Genes. 2021; 12(12):1983. https://doi.org/10.3390/genes12121983
Chicago/Turabian Styledo Nascimento, Bruna Laís Sena, Fábio Silva da Silva, Joaquim Pinto Nunes-Neto, Daniele Barbosa de Almeida Medeiros, Ana Cecília Ribeiro Cruz, Sandro Patroca da Silva, Lucas Henrique da Silva e Silva, Hamilton Antônio de Oliveira Monteiro, Daniel Damous Dias, Durval Bertram Rodrigues Vieira, and et al. 2021. "First Description of the Mitogenome and Phylogeny of Culicinae Species from the Amazon Region" Genes 12, no. 12: 1983. https://doi.org/10.3390/genes12121983